Yevgenia VASHCHYK, Roman SHCHERBYNA, Volodymyr PARCHENKO, İnna BUSHUEVA, Bogdan GUTYJ, Hanna FOTINA, Tatiana FOTINA, Yuriy STRONSKYI

PSEUDOMONAS AERUGINOSA İLE ENFEKTE EDİLMİŞ CİVCİVLERİN KARACİĞERİNE POTASYUM 2-((4-AMİNO-5-(MORFOLİNOMETİL)-4H-1,2,4-TRİAZOL-3- İL)TİYO)ASETAT (PKR-173) BİLEŞİĞİNİN DÜZELTİCİ ETKİSİNİN HİSTOLOJİK OLARAK ARAŞTIRILMASI

Amaç: Araştırmanın amacı, potasyum 2-((4-amino-5-(morfolinometil)-4H-1,2,4-triazol-3-il)tiyo)asetat(PKR-173) bileşiğinin farklı antibiyotikler ile birlikte Pseudomonas aeruginosa ile enfekte edilmiş civcivlerinkaraciğerine düzeltici etkisinin histolojik olarak incelenmesidir.Gereç ve Yöntem: Histolojik araştırma, sağlam civcivler (kontrol), Pseudomonas aeruginosa ile enfekteedilmiş civcivler (kontrol edilen patoloji), (Pseudomonas aeruginosa ile enfekte edilmeden önce) PKR-173bileşiği ile Enrofloxacin veya Saroflox gibi klasik antibiyotik yada tiotiazolin ile Enrofloxacin antibiyotiğiverilen civcivlerin karaciğeri üzerinde yapılmıştır.Sonuç ve Tartışma: Araştırmaların sonucunda; araştırılan antibiyotik olan Saroflox ile PKR-173bileşiğinin ve önleyici bir şekilde Pseudomonas aeruginosa ile enfekte edilmiş civcivlere uygulananEnrofloxacin ve Thiotriazolin- Enrofloxacin gibi klasik antibiyotiklein, nonspesifik reaktif hepatite özgü olankaraciğerdeki standart morfolojik değişiklikleri önlediği tespit edilmiştir. Pseudomonas aeruginosa ile enfekteedilmiş civcivlerin karaciğerinde gelişen patolojik sürecin kantitatif özelliklerinin analizi ve düzeltici etkisiaraştırılan metot, Saroflox antibiyotiği ile PKR-173 bileşiğinin, kontroldeki patolojiye kıyasla patolojinin bütünbelirtilerini azalttığını göstermiştir.

Anahtar Kelimeler:

hepatit, hepatoprotektif etki, 1.2.4-triazol, pseudomonas enfeksiyonu, saroflox

HISTOLOGICAL STUDY OF A CORRECTIVE INFLUENCE OF A COMPOUND POTASSIUM 2-((4-AMINO-5-(MORPHOLINOMETHYL)- 4H-1,2,4-TRIAZOL-3-YL)THIO)ACETATE (PKR-173) ON THE STATE OF CHICKEN'S LIVER UNDER INFECTION BY PSEUDOMONAS AERUGINOSA

Objectives: The aim of the research is the histological study of the corrective effect of the compound potassium 2((4-amino-5-(morpholinomethyl)-4H-1,2,4-triazol-3yl)thio)acetate (PKR-173) with different antibiotics on the state of chicken's liver under infection by Pseudomonas aeruginosa. Materials and Methods: The histological study was carried out on the levers of intact chickens (intact control); chickens that were infected by the strain of Pseudomonas aeruginosa (controlled pathology); chickens that had (before being infected by Pseudomonas aeruginosa) preventive input of the compound PKR-173 with the classic antibiotic Enrofloxacin or with Saroflox (undergoing research); or were input Thiotriazolin with the classic antibiotic Enrofloxacin. Result and Discussion: As a result, it is stated, that preventive input of the compound PKR-173 with reseachable antibiotic Saroflox, as well as with classic antibiotic Enrofloxacin and Thiotriazolin with antibiotic Enrofloxacin, to chickens infected with pseudomonas aeruginosa, prevents from chickens' livers developng stereotypical morphological changes characteristic of nonspecific reactive hepatitis. Analysis of quantitative characteristics of pathological process that developed in chicken's liver infected with pseudomonas aeruginosa, and the corrective influence of reseached methods showed that the compound PKR-173 with antibiotic Saroflox believably lowers displays of all signs of pathology compared to the controlled pathology.

Keywords:

1.2.4-triazole, hepatitis, hepatoprotective effect, pseudomonosic infection, Saroflox.,

PDF

___

1. World Health Organization. (2017). Global hepatitis report 2017. World Health Organization. 83p.
2. Rud, A.M., Kaplaushenko, A.G., Pruglo, E.S., Frolova, Y.S. (2017). Hepatoprotective activity of 1,2,4-triazole-3-thione derivatives, which contains on C5-atomic carbon hydroxy (phenyl) methyl dependent. Farmatsevtychnyi Zhurnal, (5-6), 55-63.
3. Shcherbyna, R. O., Samura, T. O., Kyrychko, B. P., Zvenihorodska, T. V., Hyrenko, I. V. (2017). The research of ammonium 2-((4-amino-5-(morpholinomethyl)-4H-1, 2, 4-triazole-3-yl) thio) acetate (PKR-177) influence on biochemical indices in rats blood under hepatitis initiated by tetrachloride methane. Zaporožskij Medicinskij Žurnal, 19(6), 819-822.
4. Al-Soud, Y. A., Al-Dweri, M. N., Al-Masoudi, N. A. (2004). Synthesis, antitumor and antiviral properties of some 1, 2, 4-triazole derivatives. Il Farmaco, 59 (10), 775-783.
5. Awad, L. F., El Sayed, H. (1998). Synthesis and conformational analysis of seco C-nucleosides and their diseco double-headed analogues of the 1, 2, 4-triazole, 1, 2, 4-triazolo [3, 4-b] 1, 3, 4-thiadiazole. Carbohydrate Research, 312(1-2), 9-22.
6. Colanceska-Ragenovic, K., Dimova, V., Kakurinov, V., Molnar, D., Buzarovska, A. (2001). Synthesis, antibacterial and antifungal activity of 4-substituted-5-aryl-1, 2, 4-triazoles. Molecules, 6(10), 815-824.
7. Demirbas, N., Karaoglu, S. A., Demirbas, A., Sancak, K. (2004). Synthesis and antimicrobial activities of some new 1-(5-phenylamino-[1, 3, 4] thiadiazol-2-yl) methyl-5-oxo-[1, 2, 4] triazole and 1-(4-phenyl-5-thioxo-[1, 2, 4] triazol-3-yl) methyl-5-oxo-[1, 2, 4] triazole derivatives. European Journal of Medicinal Chemistry, 39(9), 793-804.
8. Foroumadi, A., Mirzaei, M., Shafiee, A. (2001). Antituberculosis agents II. Evaluation of in vitro antituberculosis activity and cytotoxicity of some 2-(1-methyl-5-nitro-2-imidazolyl)-1,3,4-thiadiazole derivatives. Il Farmaco, 56(8), 621-623.
9. Labanauskas, L., Udrenaite, E., Gaidelis, P., Brukštus, A. (2004). Synthesis of 5-(2-, 3-and 4-methoxyphenyl)-4H-1,2,4-triazole-3-thiol derivatives exhibiting anti-inflammatory activity. Il Farmaco, 59(4), 255-259.
10. El-Azzouny, A., Maklad, Y., Bartsch, H., Zaghary, W., Ibrahim, W., Mohamed, M. (2003). Synthesis and pharmacological evaluation of fenamate analogues: 1, 3, 4-oxadiazol-2-ones and 1, 3, 4-oxadiazole-2-thiones. Scientia Pharmaceutica, 71(4), 331-356.
11. Grover, G., Kini, S. G. (2003). Synthesis and evaluation of new 1,3,4-oxadiazoles and 1,3,4-oxadiazole-2-thione derivatives of nalidixlc acid as potential antibacterial and antifungal agents. Indian Journal of Heterocyclic Chemistry, 12(3), 289-290.
12. Amtul, Z., Rasheed, M., Choudhary, M. I., Rosanna, S., Khan, K. M. (2004). Kinetics of novel competitive inhibitors of urease enzymes by a focused library of oxadiazoles/thiadiazoles and triazoles. Biochemical and Biophysical Research Communications, 319(3), 1053-1063.
13. Rai, K. L., Linganna, N. (2000). Synthesis and evaluation of antimitotic activity of alkylated 2-amino-1,3,4-oxadiazole derivatives. Il Farmaco, 55(5), 389-392.
14. Khan, M. T. H., Choudhary, M. I., Khan, K. M., Rani, M. (2005). Structure–activity relationships of tyrosinase inhibitory combinatorial library of 2,5-disubstituted-1,3,4-oxadiazole analogues. Bioorganic & Medicinal Chemistry, 13(10), 3385-3395.
15. Karabasanagouda, T., Adhikari, A. V., Shetty, N. S. (2007). Synthesis and antimicrobial activities of some novel 1,2,4-triazolo [3, 4-b]-1,3,4-thiadiazoles and 1,2,4-triazolo [3,4-b]-1,3,4-thiadiazines carrying thioalkyl and sulphonyl phenoxy moieties. European Journal of Medicinal chemistry, 42(4), 521-529.
16. Mathew, V., Keshavayya, J., Vaidya, V. P., Giles, D. (2007). Studies on synthesis and pharmacological activities of 3,6-disubstituted-1,2,4-triazolo [3,4-b]-1,3,4-thiadiazoles and their dihydro analogues. European Journal of Medicinal Chemistry, 42(6), 823-840.
17. Zhang Z.Y, Sun X. W. (1998). S-Triazolo[3,4-b]-1,3,4-thiadiazole derivatives. Heterocycles, 48, 561- 584.
18. Bushueva, I., Parchenko, V., Shcherbyna, R., Safonov, A., Kaplaushenko, A., Gutyj, B., Hariv, I. (2017). Tryfuzol-New Original Veterinary Drug. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, 41(1), 42-49.
19. Shcherbyna, R.O., Vashchyk, Y.V. (2018). Histological study of potassium 2-((4-amino-5-(morpholinomethyl)-4H-1, 2, 4-triazole-3-yl)thio)acetate (PKR-173) corrective influence on the chickens liver state in the condition of tetracycline hepatitis. Zaporožskij Medicinskij Žurnal, 3, 404-412.
20. Fotina T.I., Vashchyk Ye.V., Shcherbyna R.О. (2018). The problem of pseudomonas aeruginosa antibiotic- resistance and search of effective means of fighting. Veterinary Biotechnology, 32, 577-584.
21. Vashhyk Ye.V. (2009). To the study of the mechanisms of development of the infectious process in the pseudomonosis of the bird. Zb. nauk. pracj. Kharkivsjkoji DZVA, ser. Veterynarni Nauky, 20, 220-224.
22. Skrypka M.V. (1999). Pseudomonosis of poultry in Ukraine and biological properties of the pathogen, PhD Thesis, Kharkiv, 130 p.
23. Shcherbyna, R. O. (2017). Synthesis, physical and chemical properties of 2-((4-R-3-(morfolinomethylen)-4H-1, 2, 4-triazole-5-yl) thio) acetic acid salts. Current Issues in Pharmacy and Medicine: Science and Practice, 10 (1), 4-8.
24. Bovkun G.F., Borisenkova A. N. (1995). Biological properties of Pseudomonas aeruginosa and its role in pathology of poultry. Veterinariya, 32, 30-33.
25. Semyna, S. A., Sydorenko, S. V., Rezvan, S. P. (2004) Determination of the sensitivity of microorganisms to antibiotics. Methodical instructions 4.2.1890-04. Clinical Microbiology and Antimicrobial Chemotherapy, 6 (4), 306-359.
26. Ashmarin I. P., Vorob'ev L. A. (1962). Statistical methods in microbiological research. L.: Medicine, 178 p.
27. Shcherbyna, R., Vashchyk, Y. (2019). The research of 1,2,4-triazole derivatives hepatoprotective activity under tetracycline and infectious hepatitis. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, 43(2), 135-146.
28. Tiotriazolin® tablets - instructions, analogues, composition, indications, reviews. Arterium Corporation. Reference http://compendium.com.ua/info/7043/tiotriazolin-sup-sup-. Published 2019. Accessed July 20, 2019.
29. Merkulov GA. (1969). Course of pathological histology. Medicine, Moscow.343 p.
30. Sokolovskiy V.V. (1971). Histochemical studies in toxicology. Medicine, St. Petersburg. 175p.
31. Khalafyan, A. A. (2007). STATISTICA 6. Statistical analysis of data. M.: Binom-Press,512p.
32. Lapach, S. N., Chubenko, A. V., Babich, P. N. (2000). Statistical methods in biomedical research using Excel. K.: Morion, 320 p.
33. Rebrova, O. J. (2010). Statistical analysis of medical data: application of the STATISTICA software package. Moskva, Media Sfera, 312 p.
34. Khamitova, L. Y. (2015). Comparative analysis of morphofunctional indicators of the liver of doves and broilers in the critical periods of ontogenesis. Dr. biol. sci. diss., Omsk, 205p.
35. Kelberg, N. A., Sadovnikov, N. V. (2010). The role of the liver in the metabolism of birds. The morphological changes in the poultry liver while using antihomotoxic therapy. Agrarian Bulletin of the Urals, 1(67), 60-63.