Esra KÖNGÜL ŞAFAK, Selen İLGÜN, Kübra Nur ÇOBAN, Sena AKÇAKAYA MUTLU, Halil YILMAZ, Gökçe ŞEKER KARATOPRAK

MYRTUS COMMUNIS L.’NİN ANTİOKSİDAN, ENZİM İNHİBİTÖR VE SİTOTOKSİK AKTİVİTE TARAMALARI

Amaç: Bu çalışmada farklı amaçlarla geleneksel tıbbi kullanıma sahip olan Myrtus communis L.'nin biyolojik aktivitelerinin geniş bir perspektiften değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: Yapraklardan yapılan %70 metanol ekstresi’nin ve diklorometan, butanol, etil asetat ve kalan su fraksiyonlarının antioksidan (DPPH, ABTS, toplam fenol ve flavonoit miktarları), antienflamatuvar (LOX enzim inhibisyonu), antidiyabetik (α-glukozidaz enzim inhibisyonu) ve sitotoksik özellikleri (MTT testi) incelenmiştir. Sonuç ve Tartışma: En yüksek toplam fenol ve flavonoit içeriğine sahip etil asetat fraksiyonunun DPPH radikal süpürücü etkisi 1,4 µg/ml ve ABTS radikal süpürücü etkisi 2,58 mmol/lTrolox olarak bulunmuştur. LOX (IC50: 31,17 µg/ml) ve α-glukozidaz (0,5 mg/ml'de %96 inhibisyon) enzimlerinin en güçlü inhibitörü etil asetat ekstresi olarak belirlenmiştir. Diklorometan fraksiyonunun hem Hela hem de Colo hücre hatlarında en fazla sitotoksik aktiviteye sahip olduğu gösterilmiştir. Bu araştırma, tedavi edici özellikleriyle öne çıkan M. communis bitkisinin geleneksel kullanımını daha iyi anlamamızı sağlamıştır.

Anahtar Kelimeler:

Antioksidan, sitotoksik aktivite, enzim inhibisyonu, Myrtus communis

ANTIOXIDANT, ENZYME INHIBITORY, AND CYTOTOXIC ACTIVITY SCREENING OF MYRTUS COMMUNIS L.

Objective: The present study aimed to evaluate the biological activities of Myrtus communis L., which has traditional medicinal use for different purposes, from a broad perspective. Material and Method: The antioxidant (DPPH, ABTS, total phenol, and flavonoid amounts) anti-inflammatory (LOX enzyme inhibition), antidiabetic (α-glucosidase enzyme inhibition), and cytotoxic properties (MTT assay) of a 70% methanol extract made from leaves and dichloromethane, butanol, ethyl acetate, and the residual water fractions were investigated. Result and Discussion: The DPPH radical scavenging effect of the ethyl acetate fraction, with the highest total phenol and flavonoid content, was found to be 1.4 µg/ml, and the ABTS radical scavenging effect was 2.58 mmol/Trolox. The most potent inhibitor of LOX (IC50: 31.17 µg/ml) and α-glucosidase (96% inhibition at 0.5 mg/ml) enzymes was determined as ethyl acetate extract. Dichloromethane fraction was shown to have the most cytotoxic activity in both Hela and Colo cell lines. This research has given us a better understanding of the traditional use of the M. communis plant, which stands out for its therapeutic properties.

Keywords:

Antioxidant, cytotoxic activity, enzyme inhibition, Myrtus communis,

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