FLUOKSETİN VE OLANZAPİN'İN EŞ ZAMANLI SPEKTROFOTOMETRİK TAYİNİ VE YEŞİL KİMYA UYGULAMALARI

Amaç: Çalışmada fluoksetin (FLX) ve olanzapinin (OLZ) etken maddelerinin aynı anda tayini absorbans çıkarma ve absorbans düzeltme spektrofotometrik yöntemleri uygulanarak yapılmıştır. Gereç ve Yöntem: Etken maddeler absorbans çıkarma yönteminde isosbestik nokta olan 232 nm ve absorbans düzeltme yönteminde 255 nm, 245 nm dalga boyları seçilerek tayin edilmiştir. Yüzde geri kazanım çalışmaları laboratuvar karışımlarına uygulanarak yöntemlerin doğruluğu yapılmıştır. Yüzde geri kazanım değerleri OLZ için 98.1-100.2, FLX için 96.8-105.3 aralığında bulunmuştur. Çalışılan konsantrasyon aralığı, OLZ ve FLX için sırasıyla 3.12-15.62 ve 3.45-17.28 µg/ml dir. Sonuç ve Tartışma: Çalışmada antidepresan tedavisinde kullanılan iki etken madde aynı anda tayin edilmiştir. Günümüzde tedavide kulanılan bu etken maddeler tedavinin etkisinin daha iyi sağlanabilmesi için kombinasyon şeklinde kullanıma sunulmaya başlanmıştır. Bu nedenle iki etken maddenin aynı anda analizi önem kazanmaktadır. Analiz için iki farklı spektrofotometrik yöntem uygulanmıştır. Yöntemler antidepresan etken maddelerin aynı anda tayini için başarıyla uygulanmış ve valide edilmiştir. Uygulanan yöntemler ön ayırma gerektirmediği ve direkt olarak uygulanabilmesi sayesinde oluşan atık miktarı azalmaktadır. Çevreye hassas olarak yöntemler uygulanmıştır.

Anahtar Kelimeler:

Fluoksetin, kantitatif belirleme, olanzapin, spektrofotometri

SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF FLUOXETINE AND OLANZAPINE GREENNES ASSESSMENT

Objective: In our study, the simultaneous determination of fluoxetine (FLX) and olanzapine (OLZ) was performed by absorbance subtraction and absorbance correction spectrophotometric methods. Material and Method: The active substances were determined by choosing the isosbestic point of 232 nm in the absorbance subtraction method and 255 nm and 245 nm wavelengths in the absorbance correction method. The accuracy of the methods was determined by applying the percentage recovery studies to the laboratory mixtures. The percent recovery values were found in the range of 98.1-100.2 for OLZ and 96.8-105.3 for FLX. The concentration range studied was 3.12-15.62 and 3.45-17.28 µg/ml for OLZ and FLX, respectively. Result and Discussion: In the study, two active substances used in antidepressant treatment were determined simultaneously. Today, these active substances used in the treatment have started to be used in combination in order to achieve a better effect of the treatment. Therefore, simultaneous analysis of two active substances becomes important. Two different spectrophotometric methods were used for analysis. The methods have been successfully applied and validated for the simultaneous determination of antidepressant active substances. Since the applied methods do not require pre-separation and can be applied directly, the amount of waste generated is reduced. Environmentally sensitive methods have been applied.

Keywords:

Determination, fluoxetine, olanzapine, spectrophotometry,

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