Anastasiia DONCHENKO, Svitlana VASYUK, Nataliia NAHORNA

BROMOTİMOL MAVİSİ KULLANILARAK EKSTRAKSİYON OLMADAN MELOKSİKAMIN SPEKTROFOTOMETRİK TAYİNİ

Amaç: Mevcut çalışmanın amacı, bromotimol mavisi ile reaksiyona dayalı olarak meloksikamın kantitatif tayini için hızlı, basit ve hassas ekstraksiyon gerektirmeyen bir spektrofotometrik teknik geliştirmek ve doğrulamaktır. Gereç ve Yöntem: Çalışmada meloksikamın referans standardı, bromotimol mavisi ve meloksikamın bitmiş dozaj formları kullanılmıştır. Absorpsiyon ölçümleri, Analitik Jena UV-görünür spektrofotometre modeli Specord 200 üzerinde gerçekleştirilmiştir. Sonuç ve Tartışma: Geliştirilen yöntem, 348 nm’de maksimum absorpsiyon gözlenerek aseton ortamında meloksikam ve bromotimol mavisi arasındaki reaksiyonun boyalı ürününün oluşmasına dayanmaktadır. Geliştirilen teknik, özgüllük, doğrusallık, kesinlik, doğruluk, sağlamlık ve uygulama aralığı gibi validasyon özellikleri için Ukrayna Devlet Farmakopesi şartlarını karşılamaktadır. Beer yasasına uygunluk 0.80-2.40 mg/100 ml konsantrasyon aralığında gözlemlenir. Korelasyon katsayısı 0.9998’dir. Tekniğin uygulama aralığı %60-140’tır.

Anahtar Kelimeler:

Meloksikam, bromotimol mavisi, sülfonatalein boyaları, spektrofotometri, validasyon çalışmaları

EXTRACTION-FREE SPECTROPHOTOMETRIC DETERMINATION OF MELOXICAM USING BROMOTHYMOL BLUE

Objective: The purpose of the present work was to develop and validate a fast, simple and sensitive extraction-free spectrophotometric technique for the quantitative determination of meloxicam based on the reaction with bromothymol blue. Material and Method: The reference standard of meloxicam, bromothymol blue and finished dosage forms of meloxicam were used in the study. Absorption measurements were performed on the Analytic Jena UV-visible spectrophotometer model Specord 200. Result and Discussion: The developed method is based on the formation of the colored reaction product between meloxicam and bromothymol blue in acetone medium with absorption maximum at 348 nm. The method meets the requirements of the State Pharmacopoeia of Ukraine for such validation characteristics as specificity, linearity, precision, accuracy, robustness and range of application. The obedience to Beer's law is observed in the range of meloxicam concentrations 0.80-2.40 mg/100 ml, the correlation coefficient is 0.9998. The range of application of the method is 60-140%.

Keywords:

Meloxicam, bromothymol blue, sulfonephthalein dyes, spectrophotometry, validation studies,

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