Shaymaa Fadhel ABBAS ALBAAYİT, Maryam Kamel MOHAMMED

ZERUMBONE’UN ANTİANJİYOJENİK AKTİVİTESİ VE ROS ARACILI AKCİĞER KANSERİ HÜCRE HATTI HASARI

Amaç: Zerumbone (ZER), kanser önleyici etkisi olduğu bildirilen, iyi bilinen bir doğal bileşiktir. Bu nedenle, bu çalışma, ZER'in Timidin Fosforilaz'ı (TP) inhibe etme potansiyelini ve küçük hücreli dışı akciğer kanser, NCI-H460, hücre hattında Reaktif oksijen türleri (ROS) aracılı sitotoksisiteyi tetikleme yeteneğini araştırmıştır. Gereç ve Yöntem: ZER’in antianjiyogenik aktivitesi, timidin fosforilaz inhibitör testi kullanılarak değerlendirilmiiştir. Akış sitometrisi kullanılarak DCFDA boyası ile reaktif oksijen türleri (ROS) üretimi belirlenmiştir. Sonuç ve Tartışma: ZER'in 50.3±0.31 μg/ml veya 230±1.42 µM IC50 değeri ile güçlü bir TP inhibitörü olduğu bulunmuştur. ZER ile işleme tabi tutulduktan sonra NCI-H460 hücrelerinin, %55.7 gibi yüksek bir oranda ROS ürettiği bulunmuştur. Sonuç olarak, ZER’in, anti-anjiyojenik özellikler sergilediği ve akciğer kanseri hücrelerinde ROS üretimini modüle ederek kanser tedavisinde daha iyi terapötik indeks sunduğu tespit edilmiştir.

Anahtar Kelimeler:

Akış sitometrisi, NCI-H460, timidin fosforilaz

ANTIANGIOGENIC ACTIVITY AND ROS-MEDIATED LUNG CANCER CELL LINE INJURY OF ZERUMBONE

Objective: Zerumbone (ZER) is a well-known natural compound that has been reported to have anti-cancer effect. Thus, this study investigated the ZER potential to inhibit Thymidine Phosphorylase (TP) and the ability to trigger Reactive oxygen species (ROS)-mediated cytotoxicity in non-small cell lung cancer, NCI-H460, cell line. Material and Method: The antiangiogenic activity for ZER was evaluated by using the thymidine phosphorylase inhibitory test. Reactive oxygen species (ROS) production was determined via DCFDA dye by using flow cytometry. Result and Discussion: ZER was found to be potent TP inhibitory with the IC50 value of 50.3± 0.31 μg/ml or 230±1.42 µM. NCI-H460 cells upon treatment with ZER produced significant ROS by 55.7%. Consequently, ZER exerts anti-angiogenic properties and modulates ROS production in lung cancer cells, serving as leads for better therapeutic index in cancer drug.

Keywords:

Flow cytometry, NCI-H460, thymidine phosphorylase,

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