İNSAN AKCİĞER HÜCRELERİNDE ROUNDUP® TOKSİSİTESİNİN DEĞERLENDİRİLMESİ
Amaç: Bu çalışmada, en yaygın olarak kullanılan glifosat bazlı herbisitlerden (GBH) biri olan Roundup’ın, insan bronşiyal epitel hücrelerine (BEAS-2B) toksik etkileri değerlendirilmiştir.
EVALUATION OF ROUNDUP® TOXICITY IN HUMAN LUNG CELLS
Objective: In this study, toxic effects of Roundup, one of the most common glyphosate-based herbicides (GBHs), were assessed on human bronchial epithelial cells (BEAS-2B).
___
- 1. Boocock, M.R., Coggins, J.R. (1983). Kinetics of 5-enolpyruvylshikimate-3-phosphate synthase inhibition by glyphosate. FEBS Letters, 154(1), 127-133. [CrossRef]
- 2. Duke, S.O., Powles, S.B. (2008). Glyphosate: A once‐in‐a‐century herbicide. Pest Management Science, 64(4), 319-325. [CrossRef]
- 3. Williams, A.L., Watson, R.E. DeSesso, J.M. (2012). Developmental and reproductive outcomes in humans and animals after glyphosate exposure: A critical analysis. Journal of Toxicology and Environmental Health, Part B, 15(1), 39-96. [CrossRef]
- 4. Bai, S.H., Ogbourne, S.M. (2016). Glyphosate: environmental contamination, toxicity and potential risks to human health via food contamination. Environmental Science and Pollution Research, 23(19), 18988-19001. [CrossRef]
- 5. Chang, F.C., Simcik, M.F., Capel, P.D. (2011). Occurrence and fate of the herbicide glyphosate and its degradate aminomethylphosphonic acid in the atmosphere. Environmental Toxicology and Chemistry, 30(3), 548-555. [CrossRef]
- 6. Soares, D., Silva, L., Duarte, S., Pena, A., Pereira, A. (2021). Glyphosate use, toxicity and occurrence in food. Foods, 10(11), 2785. [CrossRef]
- 7. Agency for Toxic Substances and Disease Registry (ATSDR). Toxicological Profile for Glyphosate. (2020). From https://wwwn.cdc.gov/ Accessed date: 29 September 2022.
- 8. Agostini, L.P., Dettogni, R.S., Dos Reis, R.S., Stur, E., Dos Santos, E.V., Ventorim, D.P., Garcia, F.M., Cardoso, R.C., Graceli, J.B., Louro, I.D. (2020). Effects of glyphosate exposure on human health: Insights from epidemiological and in vitro studies. Science of the Total Environment, 705, 135808. [CrossRef]
- 9. Dill, G.M., Sammons, R.D., Feng, P.C., Kohn, F., Kretzmer, K., Mehrsheikh, A., Bleeke, M., Honegger, J.L., Farmer, D., Wright, D., Haupfea,r E.A. (2010). Glyphosate: discovery, development, applications, and properties. In: V.K. Nandula (Ed.) Glyphosate Resistance in Crops and Weeds: History, Development, and Management, (pp. 1-33). John Wiley & Sons, Inc.
- 10. Wang, X., Lu, Q., Guo, J., Ares, I., Martínez, M., Martínez-Larrañaga, M.R., Wang, X., Anadón, A., Martínez, M.A. (2022). Oxidative stress and metabolism: A mechanistic insight for glyphosate toxicology. Annual Review of Pharmacology and Toxicology, 62, 617-639. [CrossRef]
- 11. Mesnage, R., Bernay, B., Seralini, G.E. (2013). Ethoxylated adjuvants of glyphosate-based herbicides are active principles of human cell toxicity. Toxicology, 313(2-3), 122-128. [CrossRef]
- 12. Mesnage, R., Defarge, N., Spiroux de Vendomois, J., Seralini, G.E. (2015). Potential toxic effects of glyphosate and its commercial formulations below regulatory limits. Food and Chemical Toxicology, 84, 133-153. [CrossRef]
- 13. Martins-Gomes, C., Silva, T.L., Andreani, T., Silva, A.M. (2022). Glyphosate vs. glyphosate-based herbicides exposure: A review on their toxicity. Journal of Xenobiotics, 12(1), 21-40. [CrossRef]
- 14. International Agency for Research on Cancer (IARC). Monographs Volume 112: evaluation of five organophosphate insecticides and herbicides. (2015). From https://www.iarc.who.int/. Accessed date: 20 September 2022.
- 15. United States Environmental Protection Agency (USEPA). Revised glyphosate issue paper: Evaluation of carcinogenic potential. (2018). From https://www.regulations.gov/. Accessed date: 20 September 2022.
- 16. European Chemical Agency (ECHA). Glyphosate: No change proposed to hazard classification. (2022). From https://echa.europa.eu/. Accessed date: 3 October 2022.
- 17. Bradberry, S.M., Proudfoot, A.T., Vale, J.A. (2004). Glyphosate poisoning. Toxicological Reviews, 23(3), 159-167. [CrossRef]
- 18. Sidthilaw, S., Sapbamrer, R., Pothirat, C., Wunnapuk, K., Khacha-Ananda, S. (2022). Effects of exposure to glyphosate on oxidative stress, inflammation, and lung function in maize farmers, Northern Thailand. BMC Public Health, 22(1), 1-10. [CrossRef]
- 19. Sies, H. (1997). Oxidative stress: oxidants and antioxidants. Experimental Physiology, 82(2), 291-295. [CrossRef]
- 20. Hengartner, M.O. (2000). The biochemistry of apoptosis. Nature, 407(6805), 770-776. [CrossRef]
- 21. Chaufan, G., Coalova, I., Molina M.D.C.R.D. (2014). Glyphosate commercial formulation causes cytotoxicity, oxidative effects, and apoptosis on human cells: differences with its active ingredient. International Journal of Toxicology, 33(1), 29-38. [CrossRef]
- 22. Hao, Y., Chen, H., Xu, W., Gao, J., Yang, Y., Zhang, Y., Tao, L. (2019). Roundup confers cytotoxicity through DNA damage and Mitochondria-Associated apoptosis induction. Environmental Pollution, 252 (Pt A), 917-923. [CrossRef]
- 23. Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1-2), 55-63. [CrossRef]
- 24. Borenfreund, E., Puerner, J.A. (1985). Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicology Letters, 24(2-3), 119-124. [CrossRef]
- 25. Wang, H., Joseph, J.A. (1999). Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radical Biology and Medicine, 27(5-6), 612-616. [CrossRef]
- 26. Hao, Y., Zhang, Y., Ni, H., Gao, J., Yang, Y., Xu, W., Tao, L. (2019). Evaluation of the cytotoxic effects of glyphosate herbicides in human liver, lung, and nerve. Journal of Environmental Science and Health, Part B, 54(9), 737-744. [CrossRef]
- 27. Gasnier, C., Dumont, C., Benachour, N., Clair, E., Chagnon, M.C., Seralini, G.E. (2009). Glyphosate-based herbicides are toxic and endocrine disruptors in human cell lines. Toxicology, 262(3), 184-191. [CrossRef]
- 28. Ergun, H., Cayir, A. (2021). Exposure to glyphosate and tetrachlorvinphos induces cytotoxicity and global DNA methylation in human cells. Toxicology and Industrial Health, 37(10), 610-618. [CrossRef]
- 29. Fotakis, G., Timbrell, J.A. (2006). In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicology Letters, 160(2), 171-177. [CrossRef]
- 30. Lopes, F.M., Sandrini, J.Z., Souza, M.M. (2018). Toxicity induced by glyphosate and glyphosate-based herbicides in the zebrafish hepatocyte cell line (ZF-L). Ecotoxicology and Environmental Safety, 162, 201-207. [CrossRef]
- 31. Goulart, T.L., Boyle, R.T., Souza, M.M. (2015). Cytotoxicity of the association of pesticides Roundup Transorb(R) and Furadan 350 SC(R) on the zebrafish cell line, ZF-L. Toxicology in Vitro, 29(7), 1377-1384. [CrossRef]
- 32. Conte, F.M., Cestonaro, L.V., Piton, Y.V., Guimarães, N., Garcia, S.C., da Silva, D.D., Arbo, M.D. (2022). Toxicity of pesticides widely applied on soybean cultivation: Synergistic effects of fipronil, glyphosate and imidacloprid in HepG2 cells. Toxicology in Vitro, 84, 105446. [CrossRef]
- 33. Kwiatkowska, M., Michałowicz, J., Jarosiewicz, P., Pingot, D., Sicińska, P., Huras, B., Zakrzewski, J., Jarosiewicz, M., Bukowska, B. (2020). Evaluation of apoptotic potential of glyphosate metabolites and impurities in human peripheral blood mononuclear cells (in vitro study). Food and Chemical Toxicology, 135, 110888. [CrossRef]
- 34. Martini, C.N., Gabrielli, M., Vila Mdel, C. (2012). A commercial formulation of glyphosate inhibits proliferation and differentiation to adipocytes and induces apoptosis in 3T3-L1 fibroblasts. Toxicology in Vitro, 26(6), 1007-1013. [CrossRef]
- 35. Redza-Dutordoir, M., Averill-Bates, D.A. (2016). Activation of apoptosis signalling pathways by reactive oxygen species. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1863(12), 2977-2992. [CrossRef]
- 36. Hao, Y., Zhang, Y., Cheng, J., Xu, W., Xu, Z., Gao, J., Tao, L. (2020). Adjuvant contributes Roundup's unexpected effects on A549 cells. Environmental Research, 184, 109306. [CrossRef]
- 37. Bai, G., Zhou, R., Jiang, X., Zou, Y., Shi, B. (2022). Glyphosate‐based herbicides induces autophagy in IPEC‐J2 cells and the intervention of N‐acetylcysteine. Environmental Toxicology, 37(8):1878-1890. [CrossRef]
- 38. Silva, A.M., Martins-Gomes, C., Ferreira, S.S., Souto, E.B., Andreani, T. (2022). Molecular Physicochemical Properties of Selected Pesticides as Predictive Factors for Oxidative Stress and Apoptosis-Dependent Cell Death in Caco-2 and HepG2 Cells. International Journal of Molecular Sciences, 23(15), 8107. [CrossRef]