Hakkı ÜNLÜGENÇ, Mustafa İTEĞİN, Murat GÜNDÜZ, Geylan IŞIK

İzole sıçan frenik sinir hemidiyafragma kas preparatlarında remifentanil ve morfinin etkileri

Çalışmamızda izole sıçan frenik sinir-hemidiyafragma kas preparatlarında morfin ve remifentanilin neden olduğu kasılma ve gevşeme yanıtlarının karşılaştırılması amaçlandı. Fakülte etik kurul onayı alınarak, ağırlıkları 220-260 gr. arasında değişen 40 adet Wistar türü sıçan dekapite edilerek öldürüldükten sonra, 70-80 mg ağırlığında frenik sinir-hemidiyafragma kas preparation Kelsen ve Nochomovitz tarafından tanımlanan yönteme göre çıkarılıp hazırlandı. İzole organ banyosunda bir saatlik termoregülasyon ve dengelenme periyodundan sonra sııpramaksiınal uyarı voltajı ve optimum kas boyu (maksimum kas gerimini veren boy) hem direkt hem de indirekt yolla belirlendi. Kasılma parametreleri elde edilmeden önce preparatlar rastgele iki gruba ayrıldı. Kontrol kasılma parametreleri kaydedildikten sonra, birinci grupta (n= 20) morfin (0.1, 1, 10 ye 100 $mu$M), ikinci grupta ise remifentanil (0.001, 0.01, 0.1 ve 1 $mu$M) (n=20) banyo ortamına kümülatif dozlarda ilave edilerek kasılma parametreleri (kasılma kuvveti (KK), kasılma süresi (KS) ve yan-gevşeme süreleri (YGS) yeniden kaydedildi. izole sıçan frenik sinir-hemidiyafragma kas preparatlannda supramaksimal uyarılarla direkt ve indirekt olarak ölçülen kontrol kasılma parametrelerinden KK, KS ve YGS'nin her iki grupta benzer olduğu saptandı. Banyo ortamına morfin ve remifentanilin kümülatif ilavesiyle kasılma parametrelerinin değişiklik göstermediği ve her iki grupta benzer olduğu saptandı. Sonuç olarak; izole sıçan frenik sinir-hemidiyafragma kas preparatlannda uyguladığımız dozlarda remifentanil ve morfinin ne presinaptik (indirekt) ne de postsinaptik (direkt) depolarizasyonu etkilediği ve dolayısıyla nöromusküler kavşakta relaksasyon oluşturmadıkları kanısına varıldı.

Anahtar Kelimeler:

Kas kasılması, Nöromüsküler kavşak, Nöromüsküler blokaj, Morfin, Piperidinler, Analjezikler, opioid, Sıçan, Wistar, Kas gevşemesi

The effects of morphine and remifentanil in isolated rat pherenic nerve hemidiaphragma muscle

In our study, we aimed to investigate the effect of remifentanil and morphine on neuromuscular junction in isolated rat pherenic nerve-hemidiaphragma muscle preparation. Following approvel the protocol by animal ethic commite of Çukurova University, fourty Wistar rats (220-260 gr.) were killed by decapitation and pherenic nerve-hemidiaphragma muscle (70-80 mg weight) were isolated carefully according to the method described by Kelsen ve Nochomovitz. After the thermoregulation and equilibration period for 60 minutes, the voltage of supramaximal stimulation and optimal muscle strength were determined by both direct and indirect stimulations. Specimens were divided randomly into two groups before the contraction responses were recorded. After control contraction responses were recorded, morphine (0.1, 1, 10 and 100 $mu$M) in group I (n=20) and remifentanil (0.001, 0.01, 0.1 ve l $mu$M) in group II (n=20) were added to the bath solution cumulatively and contraction responses (contraction power: KK), contraction time: KS) and 1/2-relaxation time (YGS) were recorded again. In isolated pherenic nerve-hemidiaphragma muscle, control contraction responses contraction power (KK), contraction time (KS) and relaxation time (YGS) determined by direct and indirect supramaximal stimulations were found similar in morphine and remifentanil group. Contraction responses did not change and were found similar with the cumulative addition of opioids (morphine and remifentanil) It is concluded that remifentanil and morphine did not effect neither presinaptic (indirect) nor postsinaptic (direct) depolarization in isolated pherenic nerve-hemidiaphragma muscle produced any relaxation in neuromuscular junction

Keywords:

Muscle Contraction, Neuromuscular Junction, Neuromuscular Blockade, Morphine, Piperidines, Analgesics, Opioid, Rats, Wistar, Muscle Relaxation,

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