Batı Karadeniz Bölgesindeki entomopatojen nematodların (steinernematidae ve heterorhabditidae) tür çeşitliliği ve dağılımı

2013-2015 yılları arasında yürütülen arazi çalışmaları ile Steinernematidae ve Heterorhabditidae familyalarından entomopatojen nematodların Batı Karadeniz Bölgesinin Düzce, Bolu, Karabük ve Zonguldak illerindeki tür çeşitliliği ve dağılımı araştırılmıştır. Alınan 183 toprak örneğinin % 6’nda entomopatojen izolatına rastlanmıştır. Bu izolatların altı tanesinin moleküler analizler sonucunda, S. carpocapsae, S. feltiae, S. weiseri, türlerine ait olduğu tespit edilmiştir. Moleküler olarak tanımlanamayan diğer izolatların morfolojik gözlemlere göre üç tanesinin Steinernema, ikisinin ise Heterorhabditis izolatı olduğu görülmüştür. Nematod bulunan toprak örneklerinin tınlı, killi-tınlı ve kumlu-tınlı yapıda olduğu, toprak pH’sının 4.68 ile 7.41 arasında değiştiği görülmüştür. Steinernema türlerinin hepsi fındık, ceviz, kavak, mısır, domates, fasulye bulunan tarım alanlarından, Heterorhabditler ise orman zemininden izole edilmiştir. Çalışmada yalnızca Düzce ve Bolu illerinden entomopatojen nematod elde edilebilmiştir. Şimdiye kadar İç Anadolu ve Ege bölgelerinden bulunmuş olan S. weiseri’ye ilk defa Karadeniz bölgesinde de rastlanmıştır.

Diversity and distribution of entomopathogenic nematodes (steinernematidae and heterorhabditidae) in Blacksea Region

Diversity and distribution of entomopathogenic nematodes in the families Steinernematidae and Heterorhabditidae were assessed throughout a survey in Western Black Sea Region include Düzce, Bolu, Karabük and Zonguldak province between 2013 and 2015. Six percent of 183 soil samples were positive for presence of entomopathogenic nematode. According to molecular analyses, the isolates were identified as S. carpocapsae, S. feltiae, S. weiseri. The rest of isolates which can not be described with molecular identification methods were characterized as three Steinernema and two Heterorhabditis. Soils of the positive sites were classified as loam, sandy-loam and clay-loam and pH ranged from 4.68 to 7.41. The Steinernematids were isolated from agricultural fields include hazelnut, valnut, poplar, corn, tomato, bean, and Heterorhadits were from forest. Entomopathogenic nematodes could be isolated only Duzce and Bolu province. And S.weiseri was isolated first time from Black Sea Region.

Keywords:

Insect pathogen, Nematode Biological control,

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