Ayşe ÖZDEMİR, Utku Dönem DİLLİ, Dalyan ÖZDEMİR, Salim NEŞELİOĞLU, Özcan EREL

Kolorektal Kanserde Tiyol–Disülfit Düzeyleri

Amaç: Bu çalışmada kolorektal kanserli hastalarda natif tiyol, disülfit ve total tiyol konsantrasyonları ile CEA ve CA-19-9 serum biyobelirteçlerini değerlendirmek ve sonuçları sağlıklı bireylerle karşılaştırmak amaçlanmıştır. Gereç ve Yöntemler: Çalışmaya 23 kolorektal kanserli hasta ve 23 sağlıklı birey olmak üzere toplam 46 kişi dahil edildi. Tiyol/disülfit homeostaz testleri (natif tiyol [-SH], disülfit [-S-S] ve total tiyol [TT]) yeni bir otomatize metotla gerçekleştirildi. İskemi modifiye albümin (IMA), albümin, karsinoembriyojenik antijen (CEA), karbonhidrat antijen 19-9 (CA-19-9), TT, -SH ve -S-S seviyeleri ile disülfit/natif tiyol ve disülfit/total tiyol oranları gruplar arasında karşılaştırıldı. Bulgular: Kolorektal kanserli grupta IMA, CEA ve CA-19-9’da kontrol grubuna kıyasla istatistiksel olarak anlamlı farklılık bulundu (p<0,05). Kontrol grubunda tiyol–disülfit parametreleri ve tümör biyobelirteçleri arasında bir ilişki saptanmadı (p>0,05). Kolorektal kanserli hastalarda tiyol–disülfit parametreleri ile IMA, albümin, CEA ve CA-19-9 düzeyleri arasında bir ilişki saptanmadı (p>0,05). Tartışma ve Sonuç: Çalışmamız noninvaziv bir biyobelirteç olarak natif tiyol (-SH), disülfit (-S-S) ve total tiyol (TT) konsantrasyonları ile kolorektal kanser arasında bir ilişki olmadığını göstermektedir.

Anahtar Kelimeler:

biyokimyasal belirteç, tiyol disülfit, kolorektal neoplazmlar

The Level of Thiol-Disulfide on Colorectal Cancer

Aim: This study aimed to evaluate total thiol (TT), disulfide (-S-S), and native thiol (-SH) concentrations as serum biomarkers in patients with colorectal cancer (CRC). Materials and Methods: A total of 46 participants (23 patients with colorectal cancer and 23 healthy individuals) were included. Thiol/disulfide homeostasis tests (total thiol [TT], native thiol [-SH], and disulfide [-S-S]) were performed by a novel automated method. Ischemia modified albumin (IMA), albumin, carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA-19-9), TT, -SH, and -S-S levels as well as disulfide/native thiol and disulfide/total thiol ratios were compared between the groups. Results: In the colorectal cancer group, statistically significant difference was found in IMA, CEA, and CA-19-9, compared to the control group (p<0.05). There was no relationship between the thiol–disulfide parameters and tumor markers in the control group (p>0.05). There was no relationship between the thiol–disulfide parameters and IMA, albumin, CEA, and CA-19-9 levels in the colorectal cancer group (p>0.05). Discussion and Conclusion: Our study shows that the serum concentrations of native -SH, -S-S, and TT do not link to colorectal cancer as a noninvasive biomarker.

Keywords:

biochemical marker, colorectal neoplasms, disulfide; thiol,

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