Bazı Türk tütün çeşitlerinin isoenzim bantlarına göre tanımlanması

Bu çalışmada, bazı Türk Tütün çeşitlerinde poliakrüamid jel elektroforezi kullanılarak Eslerase ve Peroksidase isoenzimlerinin bant desenleri araştırılmıştır. N. tabucum L. türüne ait tütün çeşitleri esterase isoenzimine göre farklı bant desenleri oluşturmuştur. Peroksidase isoenzimine göre ise çeşitler arasında benzer bant desenleri görülmüştür. Birden fazla isoenzim ile çalışıldığında, isoenziınlere göre tütün çeşitlerinin tanımlanabileceği saptanmıştır.

Anahtar Kelimeler:

tütün, çeşitler, izoenzimler, elektroforez, PAGE

Identification of some Turkish tobacco varieties by isozyme banding patterns

In this study, band designs of Esterase and Peroxidase isoenzymes were investigated in some selected Turkish tobacco varieties by using polyacrilamid gel electrophoresis. Varieties belonging to N. tabacum L. generated different band designs by esterase isoenzyme. Whereas, according to peroxidase isoenzyme, similar band designs were observed. When more than one isoenzyme were studied, tobacco varieties could be identified.

Keywords:

tobacco, varieties, isoenzymes, electrophoresis, PAGE,

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