Root-knot nematode Meloidogyne arenaria infecting Swiss Chard (Beta vulgaris subsp. cicla)

Öz Swiss Chard (Beta vulgaris subsp. cicla) is a vegetable crops growing in different regions of the world for variying purposes. Root-knot nematodes (Meloidogyne spp.) seem the potantial pest group on Swiss Chard according to the reported species from other countries. In this investigation, the root knot nematode (RKN) extracted from the root of Swiss Chard was identified as M. arenaria by using different primer sets (TRNAH-MRH106 and MORF- MTHIS) targeting the large sub-unit ribosomal DNA (lrDNA) and the intergenic spacer (IGS) of rDNA of mitochondrial (mtDNA). Furthermore, the restriction enzymes (HinfI and MnlI) for the fragments given by TRNAH-MRH106 primer set was also used for restriction and digestion. Finally, species-specific SCAR primers (Far/Rar) were performed to validation of the identification results. M. arenaria is considered as an important species among the major root knot nematodes in the world. In this context, the Swiss Chard as an intercroped, small-scale produced or randomly dispersed commodity must be observed as the potantial host for the nematode. This is especially important in case of any change for long term professional production planning in the same area for more valuable agricultural commodity.

Anahtar Kelimeler:

Beta vulgaris subsp cicla, Root knot nematodes, Meloidogyne arenaria, Swiss chard

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