Pazı (Beta vulgaris subsp. cicla ) bitkisi zararlısı kök-ur nematodu Meloidogyne arenaria

Pazı (Beta vulgaris subsp. cicla), çeşitli amaçlar için dünyanın farklı bölgelerinde yetişen bir sebze türüdür. Bazı ülkelerde kök-ur nematodları (Meloidogyne spp.) pazı üzerinde potansiyel zararlı grubu olarak bildirilmiştir. Bu araştırmada, Ordu ilinde pazı köklerinden elde edilen kök-ur nematodu (RKN)’nun, mtDNA'nın bölgelerini (lrDNA ve IGS) hedefleyen farklı primer setleri (TRNAH-MRH106 ve MORF-MTHIS) kullanılarak teşhisi yapılmış ve M. arenaria olarak tanımlanmıştır. Ayrıca, TRNAHMRH106 primer setinin kullanımı ile elde edilen DNA bant büyüklüklerini kesme işlemi için HinfI ve MnlI enzimleri de kullanılmıştır. Son olarak, teşhis sonuçları türe özgü SCAR primerleri (Far/Rar) kullanılarak doğrulanmıştır. M. arenaria, dünyadaki başlıca kök-ur nematodları arasında önemli bir tür olarak kabul edilmektedir. Bu bağlamda kök-ur nematodları, küçük ölçekli üretilmiş veya rastgele dağılmış bir ürün olarak pazının potansiyel konukçusu olarak gözlenmesi gerekir. Bu durum özellikle, aynı alanda daha değerli tarımsal ürünlerin uzun vadeli profesyonel üretim planlaması durumunda herhangi bir değişiklik olması durumunda önemlidir.

Root-knot nematode Meloidogyne arenaria infecting Swiss Chard (Beta vulgaris subsp. cicla)

Swiss Chard (Beta vulgaris subsp. cicla) is avegetable crops growing in different regions of theworld for variying purposes. Root-knot nematodes(Meloidogyne spp.) seem the potantial pest group onSwiss Chard according to the reported species fromother countries. In this investigation, the root knotnematode (RKN) extracted from the root of SwissChard was identified as M. arenaria by usingdifferent primer sets (TRNAH-MRH106 and MORFMTHIS) targeting the large sub-unit ribosomal DNA(lrDNA) and the intergenic spacer (IGS) of rDNA ofmitochondrial (mtDNA). Furthermore, therestriction enzymes (HinfI and MnlI) for thefragments given by TRNAH-MRH106 primer set wasalso used for restriction and digestion. Finally,species-specific SCAR primers (Far/Rar) wereperformed to validation of the identification results.M. arenaria is considered as an important speciesamong the major root knot nematodes in the world.In this context, the Swiss Chard as an intercroped,small-scale produced or randomly dispersedcommodity must be observed as the potantial hostfor the nematode. This is especially important in caseof any change for long term professional productionplanning in the same area for more valuableagricultural commodity.

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