Hasan Murat AKSOY, Celal TUNCER, Murat ÖZTÜRK, Islam SARUHAN, Ismail ERPER, İzzet AKCA

Ölü pentatomidlerden Bacillus megaterium izolatlarının izolasyonu, karekterizasyonu ve Palomena prasina nimflerine insektisital etkisi

Bu çalışma, Bacillus megaterium izolatlarının fındık kokarcasına (Palomena prasina L., Heteroptera: Pentatomidae) karşı etkin biyokontrol ajanları olduğu gösterilmiştir. Öncelikle fındık bahçelerinde survey yapılarak, P. prasina'dan dört Bacillus megaterium izolatı elde edilmiştir. B. megaterium izolatlarının morfolojik, fizyolojik ve biyokimyasal özellikleri standart tanı yöntemlerine göre belirlenmiştir. Ayrıca izolatların tanısı için 16S rRNA gen dizi analizi yapılmıştır. 16S rRNA geninin analizi sonucu; Sa-1, Sa-5, SAk-2 ve SAkc-19 izolatlarının B. megaterium olduğu, tip ırkları ile % 100 dizi benzerliği göstermesi ile doğrulanmıştır. Bu izolatların etkinliği, laboratuarda 25±1 °C ve 70±5 RH'de P. prasina nimflerine karşı denenmiştir. İzolatlar, nimflere karşı biyolojik olarak test edilmiş ve son uygulamadan 12 gün sonra ölen bireyler günlük olarak sayılmıştır. B. megaterium izolatlarının Lethal zaman değerleri (LT50 ve LT90) hesaplanmıştır. En aktif iki izolatın LT50 değerleri 1.91 ve 11.18 gün olarak belirlenmiştir. En yüksek ölüm oranı, SAkc-2 izolatının 108 cfu ml-1 konsantrasyon uygulanması ile 12. günde % 98 olarak elde edilmiştir.

Isolation and characterization of Bacillus megaterium isolates from dead pentatomids and their insecticidal activity to Palomena prasina nymphs

Bacillus megaterium isolates, were demonstrated tobe efficient biocontrol agents against the greenshield bug (Palomena prasina L., Heteroptera:Pentatomidae). Firstly hazelnut orchards weresurveyed and four B. megaterium isolates wereobtained from P. prasina. The morphological,physiological and biochemical characteristics of B.megaterium isolates were determined according tothe standardized methodology. Additionally 16SrRNA gene sequence analyse was performed todetermine the isolates. Analysis of the 16S rRNAgene confirmed that isolates Sa-1, Sa-5, SAk-2 andSAkc-19 are B. megaterium, with 100% sequencehomology to the type strains of B. megaterium.Effectiveness of these isolates was tested against P.prasina nymphs in laboratory, at 25±1°C and 70±5RH. Isolates were bioassayed against nymphs anddead individuals were counted daily, following a 12days lasting treatment. Lethal time (LT50 and LT90)values of B. megaterium isolates were calculated.LT50 values of the two most active isolates were 1.91and 11.18 days. The highest mortality rate was 98%obtained with the treatment of SAkc-2 isolates atconcentrations of 108 cfu ml-1 on the 12th day of posttreatment.

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