Yeşil Salatalardan Pseudomonas fluorescens İzolasyonu ve İzolatların Antibiyotik Duyarlılıkları

Günümüzde yeşil salatalar genellikle pratik ve sağlıklı olduklarını düşünerek tüketilmektedir. Ancak salataların hazırlanması sırasında, salata malzemelerinin yetersiz yıkanması ve hijyenik olmayan yüzeylerle teması, salataların mikrobiyal yükünü arttırmaktadır. Bu durum salataları tüketen bireyler için sağlık sorunlarına neden olabilir. Antibiyotiklerin aşırı kullanımı, gıda kaynaklı patojenler dahil olmak üzere çoklu ilaç direncine sahip bakterilerin ortaya çıkmasına neden olmuştur. Bu patojen bakterilerin biyofilm üretme özelliklerinin olması, bu patojenlerin sebep oldukları enfeksiyonların tedavisini zorlaştırmaktadır. Bu çalışmanın amacı, farklı restoranlardan temin edilen yeşil salatalardan P. fluorescens izolasyonu ve tanımlanmasıdır. Yeşil salatalardan toplam 72 izolat izole edilmiş ve bu izolatların 29'u PZR ile Pseudomonas olarak ve 9'u P. fluorescens olarak tanımlanmıştır. Tüm P. fluorescens izolatlarının ampisilin, amoksisilin, sefuroksim, seftazidime ve seftriakson antibiyotiklerine dirençli olduğu bulunmuştur. Bu çalışmanın sonuçları, yeşil salataların hazırlanması ve depolanması sırasında hijyen koşullarına daha fazla dikkat edilmesi gerektiğini göstermektedir.

Pseudomonas fluorescens Isolation from Green Salads and Antibiotic Susceptibilities of Isolates

Currently, leafy green salads are often consumed because they are considered as practical and healthy. However,during their preparation, both inadequate washing and contact with non-hygienic surfaces may increase theirmicrobial load. This may cause several health problems for individuals consuming salads. The overuse of antibioticshas led to the emergence of multidrug resistant bacteria, including foodborne pathogens. Biofilm production ability ofthese pathogenic bacteria makes it difficult to treat infections caused by these pathogens. The aim of this study is toisolate and identify P. fluorescens from leafy green salads collected from different restaurants. A total of 72 isolateswere isolated from leafy green salads, and 29 of these isolates were identified by PCR as Pseudomonas and 9 ofthem identified as P. fluorescens. All P. fluorescens isolates were resistant to ampicillin, amoxicillin, cefuroxime,ceftazidime and ceftriaxone antibiotics. The results of this study showed that additional attention for the hygieneconditions is needed during the preparation and storage stages of leafy green salads.

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