Ahmet KOLUMAN, Sibel ÖZKÖK, Zeynep T. BURKAN

Liyofilizasyonda Kullanılan Farklı Kriyoprotektantların Salmonella typhimurium Üzerine Etkisi

Liyofilizasyon uzun süreli saklamada uygulanan bir yöntemdir. Farklı kimyasal ve çözeltiler liyofilizasyon sonrası suşların maksimum canlandırılması amacıyla kullanılmaktadır. Sakkaroz ve yağsız süt tozu en yaygın kullanılan ajanlardır. Reagent 18 genellikle farklı kültür koleksiyonlarında kullanılmaktadır. Bu araştırmada farklı kriyoprotektanlar (hücre çekirdeğini soğuktan koruyan maddeler) kullanılarak S. typhimurium liyofilize edilmiş ve suşun stabilitesi değerlendirilmiştir. Sonuçlar Reagent 18'in S. typhimurium suşlarının saklanmasında etkili olduğunu göstermiştir.

Effect of Different Cryoprotectants on Salmonella typhimurium during Freeze Drying

Freeze drying is a sophisticated way for long term storage of bacteria. Different chemicals and solutions are used to reach maximum recovery of strains after freeze-drying. Sucrose and skim milk are popular reagents that are massively used. Reagent 18 is widely used in different culture collections. In this study, different combinations of cryoprotectants are used for freeze-drying S. typhimurium, and stability of the strain was evaluated. The results showed that Reagent 18 is effective during the storage of freeze-dried S. typhimurium strains.

PDF

___

[1] Hawksworth, D.L. and Schipper, M.A.A., 1990. Criteria for consideration in the accreditation of culture collections participating in MINE, The Microbial Information Network Europe. Mircen 5: 277-281.
[2] Gams, W., Hennebert, G.L., Stalpers, J.A., Janssens, D., Schipper, M.A.A., Smith, J., Yarrow, D., Hawksworth, D.L., 1988. Structuring strain data for storage and retrieval of information on fungi and yeasts in MINE, the Microbial Information Network Europe. J. Gen. Microbiol. 134: 1667-1689.
[3] Stalpers, J.A., 1990. Structuring strain data for storage and retrieval of information on bacteria in MINE, the Microbial Information Network Europe. Syst. Appl. Microbiol. 13: 92-103.
[4] Anonymous, 1996. European Standard 1619: Biotechnology - large scale process and production -General requirements for management and organization for strain conservation procedures.
[5] Anonymous, 1996. European Standard EN 829 - Transport packages for medical and biological specimens - requirements, tests. CEN European Committee for Standardization, Brussels.
[6] Anonymous, 1999. Guidelines for the Establishment and Operation of Collections of Cultures of Microorganisms. 2nd Edition. ISBN 92 9109 043 3, Nova Publishers, NY.
[7] Ray, B., Jezeski, J.J., Busta, F.F., 1971. Repair of injury in freeze-dried Salmonella anatum. Appl. Environ. Microbiol. 22: 184-189.
[8] Mackey, B.M., Derrick, C.M., 1982. The effect of sublethal injury by heating, freezing, drying and gamma-radiation on the duration of the lag phase of Salmonella typhimurium. Appl. Environ. Microbiol. 53: 243-251.
[9] Simon, E.M., Stahl, K.L., Wilson, J.B., 1963. Preservation by freeze-drying and the stability of virulence of Salmonella typhimurium. Appl. Environ. Microbiol. 11: 371-376.