Nur Efşan KÖKSAL, İlyas ÇANKAYA, Esra ŞENGÜN ERMEYDAN

Işık Yayan Diyot Dizisi ile Işık Mikroskobunda Faz-Kontrast Görüntüleme

Bu çalışmada, aydınlık-alan, karanlık-alan ve diferansiyel faz-kontrast görüntülerini aynı anda elde edebilen tek-kameralı bir görüntüleme sistemi, programlanabilir bir ışık yayan diyot (LED) dizisi aracılığıyla farklı aydınlatma modelleri ile birlikte kullanılmıştır. Kullanılan LED dizisi, aydınlatma açılarının esnek bir şekilde modellenmesine olanak sağlamıştır. Sol-sağ, üst-alt ve eğik aydınlatmaların etkisi, hem yanak epitel hücresinde hem de soğan zarı hücresinde detaylı olarak gözlemlenmiştir. Aydınlık-alan, karanlık-alan ve diferansiyel faz-kontrast görüntüleri, 32x32 LED dizisinin desenleri değiştirilerek herhangi bir hareketli parça olmadan elde edilmiştir. Bu nedenle, kullanılan sistem basittir, düşük maliyetlidir ve geleneksel ışık mikroskobu ile uyumludur.

Anahtar Kelimeler:

Işık mikroskobu, LED dizisi, Faz-kontrast görüntüleme, 32x32 LED Matris, eğik aydınlatma

Phase-Contrast Imaging at Optical Microscope with a Light Emitting Diode Array

In this study, a single-camera imaging system that can simultaneously acquire bright-field, dark-field and differential phase-contrast images is used with different illumination patterns through a programmable light-emitting diode (LED) array. The LED array allows flexible modeling of illumination angles. The effect of left-right, top-bottom and oblique illumination was observed in detail both in the cheek epithelial cell and in the onion membrane cell. Bright-field, dark-field and differential phasecontrast images are obtained without any moving parts by changing the patterns of 32x32 LED array. Therefore, the system used is simple, low-cost and compatible with the conventional light microscopy.

Keywords:

Optical microscope, LED array, Phase-contrast imaging, 32x32 LED matrix, oblique illumination,

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