Cu(II), Ni(II), Co(II) ve Fe(II) Metaloftalosiyanintetrasülfonat Modifiye Kalem Ucu Elektrotlar ile Elektrokimyasal Dopamin Tayini

Bu çalışmada dopamin tayinlerinde kullanılmak amacıyla kalem ucu elektrot (KUE) yüzeyi Cu(II), Ni(II),Co(II) ve Fe(II) metaloftalosiyanintetrasülfonatlar ile modifiye edilmiştir. Modifiye elektrotlarkullanılarak 0,50mM dopamin içeren Britton-Robinson tamponunda (BRT) pH değeri 2,0 ile 9,0 arasındadönüşümlü voltametrik olarak yükseltgenme akım değerleri belirlenmiştir. En yüksek akım değerineCu(II) ftalosiyanintetrasülfonat ile modifiye edilmiş KUE ile (KUE/CuFSTS) BRT içinde pH 2,0’deulaşılmıştır. KUE ile kıyaslandığında KUE/CuFSTS ile elde edilen pik akımı % 30 daha fazladır. Ayrıcaoldukça düşük dopamin derişimleri için pik akımında çok daha yüksek iyileşme tespit edilmiştir. Örneğin10 µM’lık dopamin içeren BRT içinde (pH 2,0) KUE’ye kıyasla diferansiyel puls voltametrisi (DPV) ile eldeedilen yükseltgenme pik akımındaki artış 7 kattır. KUE/CuFSTS elektrotlarının performansınaadsorpsiyon süresinin etkisi de incelenmiş ve en uygun adsorpsiyon süresinin 5 dakika olduğubulunmuştur. 5 dakikalık adsorpsiyon süresiyle elde edilen elektrotların (KUE/CuFSTS-5dk)elektrokimyasal karakterizasyonu akımın difüzyon kontrollü olduğunu göstermiştir. KUE/CuFSTS-5dkelektroduna ait analitik parametreler DPV kullanılarak çalışılmıştır. DPV ölçümleri dopamin tayini içinbelirlenen eğrinin 4,0-250 µM derişimleri aralığında doğrusal olduğunu göstermiştir. Dopamin için tayinsınırı 1,3 µM (1,3 x 10-6 M) olarak bulunmuştur. Modifiye elektrotların yeniden üretilebilirliğinin bağılstandart sapması % 4,25’dir. Elde edilen sonuçlar geliştirilen modifiye elektrodun dopamin tayinlerindekullanılabilme potansiyelinin yüksek olduğunu göstermektedir.

Electrochemical Dopamine Determination by Cu(II), Ni(II), Co(II) and Fe(II) Metallophthalocyaninetetrasulfonate Modified Pencil Graphite Electrodes

In this study, pencil graphite electrode (PGE) surface was modified by Cu(II), Ni(II), Co(II) and Fe(II) metallophthalocyaninetetrasulfonates for determination of dopamine. Cyclic voltammetric oxidation current values were determined by using the modified electrodes in the Britton-Robinson buffer solution (BRT) containing 0.50 mM dopamine at the pH range of 2.0 and 9.0. The highest current value was obtained with Cu(II) phthalocyaninetetrasulfonate modified PGE (PGE/CuFSTS) in BRT at pH 2.0. The oxidation peak current value of PGE/CuFSTS is 30% higher than that of unmodified one (PGE). In addition, at very low dopamine concentrations, high peak current improvement was observed. For instance, the oxidation peak current increase obtained by using differential pulse voltammetry (DPV) is 7 fold higher than that of PGE in the BRT (pH 2.0) containing 10 µM dopamine. The effect of adsorption time on the performance of PGE/CuFSTS electrodes was also investigated and the optimum adsorption time was found as 5 minutes. The electrochemical characterization of the electrodes obtained for the 5 minutes of adsorption time (KUE/CuFSTS-5min) showed that the current was diffusion controlled. Analytical parameters of the PGE/CuFSTS-5min electrode were investigated by using DPV. DPV measurements showed that the curve for dopamine determinations are linear in the range of 4.0-250 µM. The detection limit for dopamine is 1.3 µM (1.3 x 10-6 M). The reproducibility of the modified electrodes is 4.25% (RSD). The obtained results show that the developed modified electrode has a high potential to use for dopamine detection.

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