Türk Ticari Kayısı Nektarlarının Antioksidan Aktivitesi, Toplam Fenolik Madde ve Riboflavin Profilleri

Bu çalışmada; Türk marketlerinde satılan sekiz adet kayısı nektarının toplam fenolik madde (TPs) içerikleri; antioksidan aktiviteleri ve B2 vitamini olarak bilinen Riboflavin içeriğinin tespiti yer almaktadır. Çalışmada kayısı nektarlarının toplam fenolik madde içeriklerinin tayininde Folin-Ciocalteu’s yöntemi kullanılmıştır. Antioksidan aktiviteleri için ise hem 2,2-difenil-1-pikrilhidrazil (DPPH) hem de demir indirgeme kapasitesi olarak bilinen FRAP metodları uygulanmıştır. Çalışmanın parçası olan Riboflavin içerik analizlerinde ise hızlı bir yöntem olan Laser İndüklenmiş Floresans (LIF) dedektörü varlığında Kapiler Elektroforez tekniğinden yararlanılmıştır. Çalışılan sekiz adet numunenin toplam fenolik madde içerikleri 288-680 mg gallik asit ekivalent/L aralığında tespit edilirken DPPH yöntemi ile hesaplanan % inhibasyon değerleri 30.58-98.01 aralığında; FRAP değerleri ise 1.77-5.56 mmolFe2+/L aralığında bulunmuştur. Kullanılan iki antioksidan tayini çalışmalarının birbiriyle kabul edilir orandaki uyumu hesaplanan korelasyon değeri ile (r > 0.893) de desteklenmiştir. Sekiz adet ticari kayısı nektarının Riboflavin içeriklerinin 20.50-103.37 µg/L aralığındad eğiştiği ve budurumda günlük B2 vitamini desteği için kayısı nektarından da faydalanabilineceği gözlemlenmiştir.

Antioxidant Activities, Total Phenolics and Riboflavin Profiles of Turkish Commercial Apricot Nectars

This study was performed to reveal the total phenolic (TPs) contents, antioxidantactivities and riboflavin (RF) (Vitamin B2) contents of eight commercial apricot nectarssold in Turkish markets. The total phenolic (TPs) contents of nectars were analyzedwith Folin-Ciocalteu's method. Two comparative antioxidant activities, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing capacities (FRAP) were foundfor eight nectar samples. A fast capillary electrophoretic method which is combinedwith laser-induced fluorescence (LIF) detector was applied for the riboflavin analysis.TPs values are ranged between 288-680 mg gallic acid equivalent (GAE)/L, %inhibitions in DPPH method are between 30.58-98.01% and finally FRAP values arebetween 1.77-5.56 mmol Fe2+/L. A high correlation (r > 0.893) was observed betweentwo antioxidant test systems. RF contents of apricot nectars are ranged between 20.50-103.37 µg/L. Apricot nectar juices may be considered as a good a supporting dietarysource for the daily vitamin B2 intake.

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Bhardwaj, R. L., Nandal, U., Pal, A. and Jain, S., Bioactive Compound Sand Medicinal Properties of Fruit Juices, Fruits, 69(5), 391-412, 2014.
Kalaycioglu, Z. and Erim, F.B., Total Phenolic Contents, Antioxidant Activities, and Bioactive Ingredients of Juices From Pomegranate Cultivars Worldwide, Food Chemistry, 221, 496-507, 2017.
Fan, X. G., Jiao, W. X., Wang, X. M., Cao, J. K. and Jiang, W. B., Polyphenol Composition and Antioxidant Capacity in Pulp and Peel of Apricot Fruits of Various Varieties and Maturity Stages at Harvest, International Journal of Food Science and Technology, 53(2), 327-336, 2018.
Akin, E. B., Karabulut, I. and Topcu, A., Some Compositional Properties of Mainmalatya Apricot (Prunus armeniaca L.) Varieties, Food Chemistry, 107(2), 939- 948, 2008.
Incedayi, B., Tamer, C. E, Sinir, G. O., Suna, S. and Copur, O. U., Impact of Different Drying Parameters on Color, Beta-Carotene, Antioxidant Activity and Minerals of Apricot (Prunus armeniaca L.), Food Science and Technology, 36(1), 171- 178, 2016.
Katona, Z. F., Sass, P. and Molna´r-Perl, I., Simultaneous Determination of Sugars, Sugar Alcohols, Acids and Amino Acids in Apricot by Gas ChromatographyMass Spectrometry, Journal of Chromatography A, 847, 91-102, 1999.
Bartolozzi, F., Bertazza, G., Bassi, D. and Cristoferi, G., Simultaneous Determination of Soluble Sugars and Organic Acids as Their Trimethylsilyl Derivatives in Apricot Fruits by Gas–Liquid Chromatography, Journal of Chromatography A, 758, 99-107, 1997.
Karav, S. and Eksi, A., Antioxidant Capacity and Total Phenolic Contents of Peach and Apricot Cultivars Harvested from Different Regions of Turkey, International Journal of Food and Nutrition Science, 1(4), 13-17, 2012.
Melgarejo, P., Calin-Sanchez, A., Carbonell-Barrachina, A. A, MartinezNicolas, J. J., Legua, P., Martinez, R. and Hernandez, F., Antioxidant Activity, Volatile Composition and Sensory Profile of Four New Very-Early Apricots (Prunus armeniaca L.), Journal of the Science of Food and Agriculture, 94(1), 85-94, 2014.
Korekar, G., Stobdan, T., Arora, R., Yadav, A. and Singh, S. B., Antioxidant Capacity and Phenolics Content of Apricot (Prunus armeniaca L.) Kernel as a Function of Genotype, Plant Foods For Human Nutrition, 66(4), 376-383, 2011.
Kan, T., Gundogdu, M., Ercisli, S., Muradoglu, F., Celik, F., Gecer, M. K., Kodad, O. and Zia-Ul-Haq, M., Phenolic Compounds and Vitamins in Wild and Cultivated Apricot (Prunus armeniaca L.) Fruits Grown in Irrigated and Dry Farming Conditions, Biological Research, 47(1), doi: 10.1186/0717-6287-47-46, 2014.
Gundogdu, M., Kan, T. and Gecer, M. K., Vitamins, Flavonoids, and Phenolic Acid Levels in Early- and Late-ripening Apricot (Prunus armeniaca L.) Cultivars from Turkey, HortScience, 48(6), 696-700, 2013.
Karatas, F. and Kamisli, F., Variations of Vitamins (A, C and E) and MDA in Apricots Dried in IR and Microwave, Journal of Food Engineering, 78(2), 662-668, 2007.
Kan, T. and Bostan, S. Z., Changes of Contents of Polyphenols and Vitamin a of Organic and Conventional Fresh and Dried Apricot Cultivars (Prunus armeniaca L.), World Journal of Agricultural Sciences, 6(2), 120-126, 2010.
Versari, A., Parpinello, G. P., Mattioli, A. U. and Galassi, S., Characterisation of Italian Commercial Apricot Juices by High-Performance Liquid Chromatography Analysis and Multivariate Analysis, Food Chemistry, 108, 334-340, 2008.
Castro-Puyana, M., Garcia-Canas, V., Carolina, S. and Cifuentes, A., Recent Advances in the Application of Capillary Electromigration Methods for Food Analysis and Foodomics, Electrophoresis, 33, 147-167, 2012.
Kaygusuz, H., Tezcan, F., Erim, F. B., Yildiz, O., Sahin, H., Can, Z. and Kolayli, S., Characterization of Anatolian Honeys Based on Minerals, Bioactive Components and Principal Component Analysis, LWT-Food Science and Technology, 68, 273-279, 2016.
Hashemi, P. and Erim, F. B., Analysis of Vitamin B2 in Saffron Stigmas (Crocussativus L.) by Capillary Electrophoresis Coupled with Laser-Induced Fluorescence Detector, Food Analytical Methods, 9(8), 2395-2399, 2016.
Singleton, V. L. and Rossi, J. L., Colorimetry of Total Phenolics with Phosphomolybdic-Phospho Tungstic Acid Reagents, American Journal of Enology and Viticulture, 16, 144-158, 1965.
Brand-Williams, W., Cuvelier, M. E. and Berset, C., Use of a Free Radical Method to Evaluate Antiooxidant Activity, LWT-Food Science and Technology, 28(1), 25-30, 1995.
Benzie, I. F. F. and Strain, J. J., The Ferric Reducing Ability of Plasma (FRAP) as Measure of “Antioxidant Power”: The FRAP Assay, Analytical Biochemistry, 239, 70-76, 1996.
Cataldi, T. R. I., Nardiello, D., Carrara, V., Ciriello, R. and De Benedetto, G. E., Assessment of Riboflavin and Flavin Content in Common Food Samples by Capillary Electrophoresis with Laser-Induced Fluorescence Detection, Food Chemistry, 82, 309-314, 2003.
Hu, L., Yang, X., Wang, C., Yuan, H. and Xiao, D., Determination of Riboflavin in Urine and Beverages by Capillary Electrophoresis with in-Column Optical Fiber Laser-Induced Fluorescence Detection, Journal of Chromatography B, 856, 245-251, 2007.
Tosun, I. and Ustun, N. S., An Investigation About Antioxidant Capacity of Fruit Nectars, Pakistan Journal of Nutrition, 2(1), 167-169, 2003