Zafer ŞAHİN, Alpaslan ÖZKÜRKÇÜLER, Aynur KOÇ, Hatice SOLAK, Raviye Özen KOCA, Pınar ÇAKAN, Zülfikare Işık Solak GÖRMÜŞ, Selim KUTLU

İKİ KRONİK İMMOBİLİZASYON STRES PROTOKOLÜNÜN ERKEK SIÇANLARDA DEPRESYON/ANKSİYETE BENZERİ DAVRANIŞLAR ÜZERİNE ETKİLERİNİN DEĞERLENDİRİLMESİ

Amaç: Kemirgenlerde akut ve kronik stres modellerinin depresyon ve/veya anksiyete benzeri davranış gelişimine etkilerine yönelik çalışmalar oldukça fazla olmasına karşın çelişkili sonuçlar da söz konusudur. Bu durum hayvanların cinsiyet ve yaşlarına bağlı olabileceği gibi kullanılan stres modellerinde farklılıklarla da ilişkili olabilir. Bu nedenle, söz konusu çalışmamızda erişkin erkek sıçanlarda iki immobilizasyon stres protokolünün depresyon/anksiyete benzeri davranışlara etkisinin değerlendirilmesi amaçlanmıştır.Gereç ve Yöntem: Erişkin Wistar ırkı sıçanlar kontrol, immobilizasyon stresi-1 on gün boyunca günlük 45 dakika ve immobilizasyon stresi-2 on gün boyunca günde iki kez 45 dakika olmak üzere rastgele üç gruba n= 10 ayrıldı. Stresle ilgili davranışlar açık alan testi ve zorunlu yüzme testi ile değerlendirildi. Ayrıca, vücut ağırlığı değişimi, açlık kan glikoz seviyesi ve serum kortikosteron düzeyi de ölçüldü.Bulgular: Açık alan testinde, immobilizasyon stresi-1 ve immobilizasyon stresi-2 gruplarında merkez alanda harcanan zaman yüzdesi ve ortalama hız kontrol grubuna kıyasla önemli düzeyde düşüktü sırasıyla p

An Evaluation of The Effects of Two Chronic Immobilization Stress Protocols On Depression/anxiety- Related Behavior In Male Rats

Objective: The effect of acute and chronic stress models on depression and/or anxiety-like behavior in rodents has been widely studied, but with contradictory results. This may be due to differences in the sex and age of the animals studied or inherent differences in the stress models used. Therefore, this study aimed to evaluate the effects of two immobilization stress protocols on depression/anxiety-like behaviors in adult male rats. Materials and Methods: Adult Wistar rats were randomly divided into three groups n=10 comprising: control, immobilization stress-1 45 minutes daily for a period of ten days , and immobilization stress-2 45 minutes twice a day for a period of ten days . Stress-related behavior was evaluated by means of the open field and forced swim tests. In addition, change in body weight, fasting blood glucose, and serum corticosterone were measured.Results: In the open field test, the percentage of time spent in the central area and mean velocity were significantly lower in the immobilization stress-1 and immobilization stress-2 groups as compared to the control group p < 0.05 and p < 0.01, respectively . Movement ratios were lower in both immobilization stress groups than in the control group p < 0.001 and p < 0.01, respectively . In the forced swim test, the duration of swimming, climbing and immobility behavior in both immobilization stress protocols did not differ from the control group. Serum corticosterone levels were higher in the immobilization stress-1 and immobilization stress-2 groups than in the control group p 0.05 . Conclusion: We may conclude that immobilization stress-1 and immobilization stress-2 protocols do not cause depression-like behavior in adult male rats. However, anxiety-like behaviors predominated in both stress protocol groups

Keywords:

immobilization stress, depression, anxiety, open field, forced swimming test,

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