Murat ÖZKAYA, Muharrem ÇİDEM, Mehmet ÇİDEM, Saadet Pilten GÜZEL, İlhan KARACAN

Dentin matriks protein serum düzeyi üzerine tüm vücut vibrasyonun etkisi

AMAÇ: Dentin matriks protein DMP osteositlerde eksprese edilen ekstraselüler maktriks ile ilişkili mekanosensitif bir fosfoproteindir. Bu araştırmanın amacı, genç erişkin sağlıklı erkeklerde DMP serum düzeyinin tüm vücut vibrasyon TVV ile değişip değişmediğini ortaya koymaktır.YÖNTEMLER: Araştırmaya, 20-45 yaş arasında 8 sağlıklı gönüllü erkek denekler alındı. Deneklere TVV platformu üzerinde ayakta dik postürde dururken frekansı 35 Hz, tepe-tepe amplitüdü 2.2 mm, süresi 45 sn olan vibrasyon uygulandı. TVV öncesi bir kez ve vibrasyon seti sona erdikten sonra ikişer dakika ara ile 10 kez TVV’dan hemen sonra, 2.dk, 4.dk, 6.dk, 8.dk, 10.dk, 12.dk 14.dk, 16.dk ve 18.dk kan örneği alındı. Ölçümler bir araştırmacı tarafından, numunelere kör olarak yapıldı. Serum DMP düzeyi Human Dentin Matrix Protein ELISA kiti ile ölçüldü.BULGULAR: TVV öncesi serum DMP düzeyi 22.5 ± 5.8 ng/ml idi ve TVV uygulaması ile anlamlı değişim olmadığı saptandı F 2.2-13.3 = 1.45 P=0.271 .SONUÇ: Sonuç olarak, bu araştırma sağlıklı genç erişkin erkeklerde DMP serum düzeyinin erken dönemde TVV sonrası ilk 18 dakikada değişmediğini ortaya koymaktadır.

Anahtar Kelimeler:

Dentin matriks protein, kemik, osteosit, vibrasyon

Effect of whole body vibration on the serum dentin matrix protein level

OBJECTIVE: Dentin matrix protein DMP is a mechanosensitive phosphoprotein expressed in osteocytes and associated with the extracellular matrix. The aim of this study was to determine whether serum DMP levels were affected by applying whole-body vibration WBV in healthy young adults.METHODS: Healthy eight volunteer men 20–45 years were recruited for the study. WBV was applied to the experimental group at frequency of 35 Hz, 2.2 mm peak-to-peak amplitude and duration of 45 seconds while standing the upright posture on the platform. Serum DMP levels was measured before and on the first, second, sixth, eighth, tenth, twelfth, fourteenth, sixteenth ve eightheenth minute after vibration. A researcher was blind to the measurement of samples. Serum DMP level were measured by Human Dentin Matrix Protein ELISA kit.RESULTS: Serum DMP level before WBV was 22.5 ± 5.8 ng / ml and there was no significant change after WBV F 2.2-13.3 = 1.45 P = 0.271 .CONCLUSION: In conclusion, the findings of this study reveals that serum DMP level in the healthy young adult men did not change in early period first 18 minutes after WBV .

Keywords:

Bone, Dentin matrix protein, Osteocyte, Vibration,

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