ZELİHA UĞUR AYDIN, KEREM ENGİN AKPINAR, CEYLAN HEPOKUR, Merve ALPAY, DEMET ALTUNBAŞ

Effect of various irrigation solutions on cytokine expression of human gingival fibroblast: in vitro study

Aim: The aim of this study is to investigate the release of IL-1, TNF- α and VEGF following administration of NaOCl, propolis and chitosan solutions on human gingival fibroblasts. Materials and Methods: This study was conducted on human fibroblast by cell culture and evaluation of the direct effect of various solutions on the cultured cells. The release of pro-inflammatory interleukin (IL), tumor necrosis factor (TNF) α and vascular endothelial growth factor (VEGF) on fibroblast was analyzed after administration of irrigation solutions. Log concentrations of NaOCl, Propolis and Chitosan effects on cells were measured by colorimetric method. Results: IL-1 and TNF-α secretion levels decreased during propolis and chitosan applications, which are natural products. It was also found that the propolis increased VEGF secretion more than the other materials. Conclusions: The results of this study suggest that propolis and chitosan may contribute to the recovery of periapical tissues via anti-inflammatory cytokines level secreted during the inflammatory process. It is important to search for the biological effect of the materials in contact with the direct or indirect cause of the surrounding tissues during the endodontic treatment.

Farklı irrigasyon solüsyonlarının insan fibroblast hücreleri üzerinde sitokin ekspresyonuna etkisi: in vitro çalışma

Amaç: Bu çalışmanın amacı human fibroblast hüre hattı üzerinde NaOCl, propolis ve kitosan solüsyonlarının uygulanmasının ardından IL-1, Tnf-a ve VEGF salınımını araştırmaktır. Gereç ve Yöntem: Bu çalışma, insan fibroblast hücre hattı üzerinde farklı irrigasyon solüsyonlarının doğrudan uygulanmasının ardından oluşan etkinin değerlendirilmesiyle gerçekleştirilmiştir. İrrigasyon solüsyonlarının uygulanmasının ardından fibroblast hücresi üzerindeki pro-inflamatuar interlökin (IL), tümör nekrozis faktörünün (TNF) α ve vasküler endotelyal büyüme faktörünün (VEGF) salınımı analiz edildi. Hücreler üzerindeki NaOCI, Propolis ve Kitosan etkilerinin log konsantrasyonları kolorimetrik yöntemle ölçüldü. Bulgular: Doğal materyaller grubunda bulunan propolis ve kitosan solüsyonlarının uygulaması ile IL-1 ve TNF-α sekresyonu azaldı(p. Propolisin VEGF sekresyonunu diğer solüsyonlardan daha fazla artırdığı bulundu(p

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