RESVERATROL YÜKLÜ SELÜLOZ ASETAT LİFLERİNİN KARAKTERİZASYONU

Bu çalışmada, selüloz asetat çözeltisinden elektroeğirme prosesi ile enkapsülasyon matrisleri üretilmiş ve bu matrislerin resveratrolün enkapsülasyonundaki performansları incelenmiştir. Taramalı elektron mikroskobu (SEM) ile incelendiğinde, selüloz asetat liflerinin düzgün yüzeyli olduğu ve damlacık içermediği görülmüştür. Resveratrol yüklü liflerin ve yüksüz liflerin ortalama çapları sırasıyla 493 nm ve 478 nm olarak belirlenmiştir. Resveratrolün enkapsülasyon etkinliği %94.5 olarak bulunmuştur. Antioksidan aktivite analizi, depolama sırasında elektroeğrilmiş liflere enkapsülasyon ile biyoaktif bileşiğin bozunmasının azaldığını ortaya koymuştur. In vitro salınım çalışması, 20 saat içerisinde başlangıçtaki resveratrolün %11’inin tampon ortamına salındığını ve sonrasında salınımın durduğunu göstermiştir. Sonuç olarak, elektroeğrilmiş selüloz asetat liflerinin resveratrol için etkin enkapsülasyon matrisleri olarak kullanılma potansiyeli olduğu belirlenmiştir.

Anahtar Kelimeler:

Resveratrol, selüloz asetat, elektroeğirme, fiber, enkapsülasyon

CHARACTERIZATION OF RESVERATROL LOADED CELLULOSE ACETATE FIBERS

In this work, encapsulation matrices were fabricated by electrospinning of cellulose acetate solution and their performance for the encapsulation of resveratrol was evaluated. Scanning electron microscopy (SEM) results showed that cellulose acetate fibers were bead-free and smooth. The mean diameters of resveratrol loaded and unloaded fibers were found to be 493 nm and 478 nm, respectively. Encapsulation efficiency of resveratrol was 95%. Antioxidant activity assay confirmed that the encapsulation via electrospun fibers reduced the degradation of bioactive compounds during storage. In-vitro release study showed that 11% of the resveratrol in cellulose acetate fibers was released into buffer medium within 20 h and after that, the release reached plateau. Therefore, electrospun cellulose acetate fibers are promising to be used as an efficient encapsulating matrices for resveratrol.

Keywords:

Resveratrol, cellulose acetate, electrospinning, fiber, encapsulation,

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