Cem FIÇICIOĞLU, Tülay İREZ, Ayça ÇUBUKÇU, Serap YALTI, Birgül GÜRBÜZ, EBRU ÇÖĞENDEZ

The relation of sperm function tests and fertilization failure in invitro fertilization

Amaç: Sperm fonksiyon testlerinin fertilizasyon başarısızlığına etkisi Metod: Spontan ve stimule akrosom reaksiyon (AK) and Hypoosmotik şişme testi (HOS) normospermik örneklerde uygulandı. Ca ionophore, A23187 maksimal acrosome kaybı ve hypoosmotic medyum (Fructose, Na citrate 150 mOsm) spermatozoa'yı şişirmek için sırası ile kullanıldı. Bulgular: İndüklenmiş AR, spontan AR sonuçlarına göre daha yüksek fertilizasyon başarı gösterdi. HOS grade l-4 ile fertilizasyon oranlan arasında anlamlı ilişki bulundu Sonuç: Stimulate AR testi ve HOS test dereceleri IVF'de fertilizasyon olup olmayacağını önceden tahmin etmede önemli role sahiptir.

Anahtar Kelimeler:

İnvitro fertilizasyon, Sperm, Akrosom reaksiyonu, Tedavi başarısızlığı

Sperm fonksiyon testlerinin in vitro fertilizasyon uygulamalarında fertilizasyon başarısızlığına etkisi

Objective:To investigatethe effects of sperm function test on IVF failure. Methods: In this study spontaneous and the stimulated acrosome reaction (AR) and Hypoosmotic swelling test (HOS) was performed for normospermic samples. Ca ionophore was A23187 used for maximal acrosome loss and hypoosmotic media (Fructose,Na cit-rate 150 mOsm) for swelling of spermatozoa, respec-tively. Results: We found that induced AR results had higher fertilization success than spontaneous AR results and a significant correlation between HOS grade 1-4 and fertilization rates. Conclusion: Stimulated AR test and HOS test grades can predict sperm fertilization ability in IVF.

Keywords:

Fertilization in Vitro, Spermatozoa, Acrosome Reaction, Treatment Failure,

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