Yunus ÇETİN, İbrahim H. YÖRÜK, İbrahim TAŞAL

Keçilerde gebelik süresince retinol, $alpha$ tokoferol ve vitamin D3 konsantrasyonlarının araştırılması

Bu çalısmada tiftik keçilerinde retinol, a-tokoferol ve vitamin D3 (kolekalsiferol) konsantrasyonlarının gebelik dönemi olası degisimlerinin belirlenmesi amaçlanmıstır. Arastırmada saglıklı, östrüsleri PGF2 a ile senkronize edilmis, yasları 2–4 arasında degisen 12 adet renkli Tiftik keçisi kullanıldı. Çalısmada 150 günlük bir süreçte toplam 10 defa kan alındı. Gebelik boyunca retinol ve a-tokoferol arasında önemli korelasyon bulunurken, vitamin D3 seviyelerinin küçük dalgalanmalar dısında istikrarlı bir seyir izledigi görüldü. Sunulan arastırma ile keçilerde gebelik boyunca a-tokoferol, retinol ve vitamin D3 düzeyleri ortaya konuldu. Keçilerde konunun yeterince arastırılmadıgı, anılan vitaminlerin mevsimsel degisimi, beslemede kullanılan rasyonun ve laktasyon döneminin bu vitaminlere etkileri üzerine arastırmalar yapılması gerektigi kanısına varıldı.

Determination of retinol, $alpha$ tocopherol and vitamin D3 levels during pregnancy in goats

The purpose of the present study was to determine whether significant changes in sera levels of retinol, a-tocopherol and vitamin D3 during pregnancy in goats. The animal material of the study consisted of 12 healthy Mohair goats, between 2 and 4 years old. Oestrus was synchronized using dual dose PGF2 a at 11 days interval. A significant correlation was found between retinol and a-tocopherol throughout the pregnancy. No significant fluctuations were observed at vitamin D3 levels during pregnancy. In conclusion, the results of this study are shows sera levels of a-tocopherol, retinol and vitamin D3 in pregnant goats. There is little information regarding the effects of seasonal changes, pregnancy, ration and lactation on these vitamins in goats and new studies should be performed by researchers.

PDF

___

1. Aksakal M, Karakılçık AZ, Kalkan C, Çay M, Nazıroglu M (1995): İneklerde üremenin çesitli dönemlerinde bkaroten ve E vitamini düzeyleri. Doga Tr. J. Vet. Anim. Sci. 19: 59–64.
2. Blomhoff R, Blomhoff HK (2006): Overview of retinoid metabolism and function. J. Neurobiol. 66: 606–630.
3. Çetinkaya N, Ulutürk S, Öztürk A (1996): Ankara keçilerinde serum retinol ve beta-karoten seviyelerinin mevsimsel degisimleri. Lalahan Hay. Ars. Ens. Der., 36: 88-94.
4. Dede S, Deger Y, Kahraman T, Deger S, Alkan M, Cemek M (2002): Oxidation Products of Nitric Oxide and the Concentrations of Antioxidant Vitamins in Parasitized Goats. Acta Vet. Brno. 71: 341–345.
5. Goff JP, Kimura K, Horst RL (2002): Effect of mastectomy on milk fever, energy, and vitamins A, E, and b- Carotene status at parturition. J. Dairy Sci. 85: 1427–1436.
6. Fechner H, Schlamet M, Guthmann F, Stevens PA and Rüstow B (1998): İ- and İ- Tocopherol Induce Expression of Hepatic İ-Tocopherol Transfer-Protein mRNA. Biochem.J., 331, 577-581.
7. Horst RL, Goff JP, Reinhardt TA (1994): Calcium and vitamin D matabolism in the dairy cow. J. Dairy Sci. 77:1936–1951.
8. Kara H, Karatas F, Servi K, Akar Y, Konar V (2001): Holstayn sıgırlarda gebeligin farklı dönemlerindeki plazma adrenalin, noradrenalin ile vitamin A ve E düzeylerinin arastırılması. F.Ü. Sag. Bil. Derg. 15: 171–174.
9. Katsoulos PD, Roubies N, Panousis N, Karatzanos P, Karatzias H (2005): Long-term fluctuations and effect of age on serum concentrations of certain fat-soluble vitamins in dairy cows. Vet. Clin. Pathol. 34: 362–367.
10. Miller KW and Yang CS (1985): An Isocratic High- Performance Liquid Chromatography Method for the Simultaneous Analysis of Plasma Retinol, İ-tocopherol and Various Carotenoids. Analitical Biochemistry 145: 21-26
11. LeBlanc, S.J., Herdt, T.H., Seymour, W.M., Duffield, T.F., Leslie, K.E. (2004): Peripartum serum vitamin E, retinol, and beta-carotene in dairy cattle and their associations with disease. J. Dairy Sci., 87: 609-619.
12. Liesegang A, Risteli J, Wanner M (2006): The effects of first gestation and lactation on bone metabolism in dairy goats and milk sheep. Bone 38: 794–802.
13. Meglia EG, Jensen SK, Lauridsen C, Waller P (2006): a- Tocopherol concentration and stereoisomer composition in plasma and milk from dairy cows fed natural or synthetic vitamin E around calving. J. Dairy Res. 73: 227–234.
14. Paulson SK, Langman CB (1990): Plasma vitamin D metabolite levels in pregnant and nonpregnant ewes. Comp. Biochem. Physiol. A: Physiology 96: 347–349.
15. Paulson SK, Deluca HF (1986): Review article: Vitamin D metabolism during pregnancy. Bone 7: 331–336.
16. Reynolds SL and Judd HJ (1984): Rapid Procedure for the Determination of Vitamins A and D in Fortified Skimmed Milk Powder Using High-Performance Liquid Chromatography. Analyst 109: 489-492
17. Yokus B, Cakır DU, Kanay Z, Gulten T, Uysal E (2006): Effects of seasonal and physiological variations on the serum chemistry, vitamins and thyroid hormone concentrations in sheep. J. Vet. Med. A 53: 271-276.
18. Yur F, Dede S, Deger Y, Mert N (2003): Effects of gestation and lactation on lipid peroxidation and antioxidant system in sheep. Indian Vet. J. 80: 997–998.
19. Zanker IA, Hammon HM, Blum JW (2000): Beta-carotene, retinol and alpha-tocopherol status in calves fed the first colostrum at 0-2, 6-7, 12-13 or 24-25 hours after birth. Int. J. Vitam. Nutr. Res. 70: 305–310.
20. Zile MH (2001): Function of vitamin A vertebrate embryonic development. J. Nutr. 131: 705–708.