Yaralıoğlu Sema GÜRGÖZE, NİHAT ŞINDAK, Seval YILMAZ, Hasan SERTKAYA, Temizer Sema OZAN

Bursitis prekarpalisli sığırlarda kortikosteroid tedavsininin bazı intioksidan enzim ve lipid peroksidasyon seviyeleri üzerine etkileri

Bu çalışma, sığırlarda akut seröz nitelikteki bursitis prekarpalis olgularında kortikosteroid tedavisinin antioksidan enzim (glutatyon peroksidaz, GSH-Px; katalaz, ÇAT), lipid peroksidasyon (MDA) ve glutatyon (GSH) seviyeleri üzerine etkilerini araştırmak amacıyla yapıldı. Çalışmanın materyalini Şanlıurfa 'mn Ceylanpınar ilçesi Tarım İşletmelerinde bulunan 9 'u bursitis prekarpalisli ve 9 'u da sağlıklı olmak üzere toplam 18 adet Holştayn ırkı sığır oluşturdu. Bursal keseye iki gün süreyle vezikan pomat uygulamalarından sonra kese içerisindeki synoviyal sıvı boşaltılarak antiseptik ile irrigasyonu takiben intrabursal kortikosteroid ve antibiyotik uygulamaları yapıldı. Hasta hayvanlardan tedaviden önce bir (birinci gün) tedaviden sonra ise iki kez (ikinci gün ve 21. gün) kan örnekleri alındı. Bursitisin sığırlarda önemli derecede eritrosit ÇAT ve GSH düzeylerinin düşmesine, MDA düzeyinde ise artışa neden olduğu tespit edildi. Kortikosteroidlerle tedavinin GSH-Px düzeyinde önemli bir artışa neden olduğu belirlendi. Tedavi öncesine kıyasla tedavi sonrası MDA düzeyinde azalma, GSH düzeyinde ise anma saptandı. Sonuç olarak; bursitisli sığırlarda kortikosteroid tedavisinin oksidan düzeyini düşürüp antioksidan sistemi stimüle ederek ve peroksidasyon reaksiyonlarını değiştirerek etki oluşturabileceği kanaatine varıldı.

Anahtar Kelimeler:

katalaz, lipit peroksidasyonu, enzimler, antioksidanlar, glutatyon peroksidaz, sığır, glutatyon, kortikoidler, sığır hastalıkları

The effects of corticosteroid treatment on some antio idant enzymes and the level of lipid peroxidation in cattles with burcitis precarpalis

This study was conducted to access the effect of corticosteroid treatment on the activity of antioxidant enzymes (glutathione peroxidase-GSH-Px, catalase-CAT), the levels of lipid peroxidation (MDA) and glutathione (GSH) in catties with acut serous burcitis precarpalis. Nine healthy and nine with burcitis precarpalis totally 18 Holstain breed cattle from Ceylanpınar district, Şanlıurfa was choosen for the study. The sinovial liquid, formed after vezikan pomad application for two days, was discharged antiseptically and the intrabursal corticosteroid and antibiotic regimen was applied. Blood samples were drawn from the cow once before and twice (two and 21 days) after the treatment. Burcitis caused significant decreased in erythrocyte CAT and GSH levels. while significant increased in the level of MDA. After treatment corticosteroids caused a significant increases in the level of GSH-Px. The MDA level decreased while the GSH was increased after the treatment. As a result, we believe that corticosteroid theraphy in bursitic catties, induces antioxidant systems and changes the peroxidation reactions.

Keywords:

catalase, lipid peroxidation, enzymes, antioxidants, glutathione peroxidase, cattle, glutathione, corticoids, cattle diseases,

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