Yetiştiriciliği Yapılan Bazı Türk Kişniş (Coriandrum Sativum L.) Çeşitlerinde Genetik Çeşitliliğin ISSR ve SRAP Marköleri Yardımıyla Değerlendirilmesi

Coriandrum sativum L., Umbelliferae / Apiaceae ailesine ait uçucu yağ dahil olmak üzere tıbbi bitki olarak kullanılan bir baharat bitkisidir. Farklı kısımlarındaki çeşitli kimyasal bileşenleri, antibakteriyel, antifungal ve antioksidatif olarak kullanışlıdır. Dolayısıyla gıdaların bozulmasını önleyerek raf ömrünün uzatılmasında önemli bir rol oynamaktadır. Kişnişin yaprak ve tohumları çoğunlukla geleneksel tedavide kullanılır ve uçucu yağları insanlar için toksik olmaması nedeniyle farmasötik ürünler ve parfüm bileşenlerinde yer alır. Kişniş, moleküler olarak yeterince tanımlanmamıştır. Kişniş ıslah çalışmalarıyla genetik olarak tek düze Coriandrum sativum L çeşitlerine ulaşılabilecektir. Kabul edilebilir Genotipler, germplazmdaki genetik değişkenlik miktarıyla belirlenir ve kişniş için kullanılabilir bilgiler sınırlıdır. Genetik farklılığının belirlenmesinin çalışılması ve ıslah programlarında uygun genotip kullanımının öneminin vurgulanması, kişnişteki genetik tabanı genişletmek için gereklidir. Bu çalışmada; bazı Türk kişniş çeşitleri arasındaki genetik varyasyonlar iki farklı markör tekniği kullanılarak (ISSR ve SRAP) DNA düzeyinde araştırılmış ve ticareti yapılan bu çeşitler arasındaki genetik ilişkinin belirlenmesi amaçlanmıştır. Her iki primer toplam 473 skorlanabilir bant vermiş ve her markörden elde edilen sonuçlar genotipler arasındaki filogenetik ilişkileri göstermiştir.

Anahtar Kelimeler:

ISSR, SRAP, Coriandrum sativum L., Moleküler markör, SRAP

Assessment of Genetic Variation on Some Cultivated Turkish Coriander (Coriandrum Sativum L.) Varieties Based on ISSR and SRAP Markers

Coriandrum sativum L. is the spices plant that used as medicinal plant including essential oil, belonging to the family Umbelliferae/Apiaceae. Its various chemical components in different parts are useful as antibacterial, antifungal and antioxidative. Therefore plays a major role in preserving the shelf life of foods by preventing their spoilage. Leaves and seeds of coriander mostly used in folk medicine and its essential oil used for pharmaceutical products and as an ingredient in perfumes cause of non-toxic to humans. Coriander is not much defined as molecularly. Coriander breeding studies will probably result in more or less genetically identical varieties of Coriandrum sativum L. The acceptability of the genotypes is determined by the amount of genetic variability in the germplasm and the utilizable information is limited in coriander. Studying to determine the genetic diversity of identified characters and emphasis of importance to use convenient genotype in a breeding program is needed in order to enlarge the genetic base in coriander. In this study; Genetic variations among some Turkish coriander varieties were investigated at the DNA level using two different marker techniques (ISSR and SRAP) and the aim was to determine the genetic relationship between these traded varieties. Both primers gave totally 473 scorable bands and all results from each marker have shown the phylogenetic relationships between genotypes.

Keywords:

ISSR, SRAP, Coriandrum sativum L., Molecular marker, SRAP,

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