Hakime Hülya ORAK, Magdalena KARAMAĆ, Adnan ORAK, Hazım Serkan TENİKECİER, Ryszard AMAROWİCZ

Türkiye’de Yetiştirilen Farklı Yonca (Medicago sativa L.) Çeşitlerinin Antioksidan Aktiviteleri ve Toplam Fenolik Madde İçeriği

Bu çalışmada Türkiye'de yetiştirilen 15 yonca çeşidi, antioksidan potansiyellerini belirlemek ve çeşitler arasındaki farklılıkları karşılaştırmak amacıyla araştırılmıştır. Yonca tohumu metanol ekstraktlarında toplam fenolik madde içeriği, DPPH•, ABTS•+ giderim ve FRAP aktiviteleri belirlenmiştir. Ekstrelerin TP değerleri 37.03 mg GAE/g ile 54.04 mg GAE/g arasında bulunmuştur. TEAC değerleri 0.100 mmol Trolox/g ile 0.158 mmol Trolox/g arasında değişmiştir. FRAP aktivitesi 389.90 µmol Fe2+/g'den 791.02 µmol Fe2+/g aralığında değişim göstermiştir. Sonuçlar, incelenen karakterler açısından yonca çeşitleri arasındaki farklılıkların istatistiksel olarak önemli (p < 0.01) olduğunu göstermektedir.

Anahtar Kelimeler:

Alfalfa, Medicago sativa L., Fenolik içerik, Tohum, Çeşit, Antioksidan aktivite

Antioxidant Activity and Total Phenolic Contents of Different Alfalfa (Medicago sativa L.) Varieties Grown in Turkey

Fifteen alfalfa varieties cultivated in Turkey were researched to compare the antioxidant potential of their seeds and find differences between the varieties. The total phenolic (TP) content, DPPH•, and ABTS•+ scavenging activities, and ferric-reducing antioxidant power (FRAP) of alfalfa seed methanol extracts were determined. TP contents of extracts were found between 37.03 and 54.04 mg GAE/g. ABTS assay results ranged between 0.100 and 0.158 mmol Trolox/g extract, and FRAP changed from 389.90 to 791.02 µmol Fe2+/g extract. The results demonstrated that the differences between the alfalfa varieties were significant (p < 0.01) in terms of analyzed characters.

Keywords:

Alfalfa, Antioxidant activity, Medicago sativa L., Phenolic contents, Seed, Variety,

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