Omid Sofalian, Pooran BADARİAN, Ali ASGHARI, Mohammad SEDGHI, Behman FIROOZİ

SDS-Page Tekniği Kullanılarak Bazı Soya (Gylicine max Merril) Genotiplerindeki Tohum Depo Protein Polimorfizminin Belirlenmesi

Tohum depo proteinleri, çevre tarafından daha az etkilenir ve yüksek tekrarlanabilirliğe sahiptir ve kültür bitkilerinde genotipleri arasındaki genetik varyasyonum belirlenmesinde ve aynı zamanda belirli ürün türlerinin çeşitlerinin ayırt edilmesinde güçlü bir araç haline gelen bir biyokimyasal belirteç olarak kabul edilmektedir. Bu çalışmada, 17 soya genotipi SDS-PAGE kullanılarak incelenmiştir. Sonuçlar, toplam protein ve düşük moleküler ağırlıklı (LMW-GS) lokuslar dahil olmak üzere tespit edilen tüm lokusların genetik çeşitlilik ortalamasının, 0.48 olduğunu ve yüksek molekül ağırlığına sahip proteinler (HMW-GS) için ise 0.42 olduğunu göstermiştir. Ayrıca, Shannon ortalama endeksi, bu üç depo protein grubu için sırasıyla 0.4096, 0.41 ve 0.60 olarak bulunmuştur. Toplam proteinler için oluşturulan kümeleme analizi genotiplerin dört gruba ayrırırken, genotipler düşük moleküler ağırlıklı (LMW-GS) ve yüksek moleküler ağırlıklı (HMW-GS) sahip proteinler bakımından üç gruba ayrılmıştır. Bu polimorfizm, herhangi bir biyokimyasal belirteç tabanlı ıslah programlarında seleksiyon amacı ile kullanılabilecektir.

Anahtar Kelimeler:

Kümeleme analizi, Genetik çeşitlilik, SDS-PAGE, Soya

Identification of Seed Storage Protein Polymorphism in Some Soybean (Glycine max Merril) Genotypes Using SDS-PAGE Technique

Seed storage proteins are considered as biochemical markers, which are less affected by the environment and have high repeatability, and have become a powerful tool in studying genetic variation within and among genotypes in crop plants and also distinguishing cultivars of a particular crop species. In this study, 17 genotypes of soybean were studied using SDS-PAGE. The results showed that genetic diversity average for all detected loci including total proteins and low molecular weight (LMW-GS) was equal to 0.48 and for proteins with high molecular weight (HMW-GS) was 0.42. Additionally, Shannon average index were 0.4096, 0.41 and 0.60 respectively with regard to three storage proteins examined. While the cluster analysis produced from total proteins divided genotypes into four groups, proteins with low molecular weight (LMW-GS) and high molecular weight(HMW-GS) divided them into three groups. This polymorphism could be used in any biochemical marker based breeding programs to facilitate selection.

Keywords:

Cluster analysis, Genetic diversity, SDS-PAGE, Soybean,

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