Morphogenetic, Ontogenetic and Diurnal Variability in Antioxidant Activity, Total Phenol and Flavonoids of Foeniculum vulgare Miller var. vulgare Extracts
Morphogenetic, Ontogenetic and Diurnal Variability in Antioxidant Activity, Total Phenol and Flavonoids of Foeniculum vulgare Miller var. vulgare Extracts
Bioactive compounds, appearing at varying quantities and constituents in medicinal plants, act asnatural antioxidants. The aim of this study was to monitor morphogenetic, ontogenetic and diurnal variability inantioxidant activity, total phenols and total flavonoids of bitter fennel extracts. Morphogenetic variability wasassessed using leaf and root-bulb-stem samples of pre-, full- and post-flowering stages, flower samples of fullflowering and seed samples of seed formation and harvest maturity. To monitor diurnal (3 times a day) andontogenetic variability, whole plant samples were taken at pre-, full- and post-flowering. The plant extracts wereobtained by methanol solution and radical scavenging effect of the extracts were determinedspectrophotometrically. The root-bulb-stem extract produced the highest total phenol content. The highestradical-scavenging activity, with the lowest IC50 = 24.17 μg ml –1, was obtained from the seed extract. Totalphenols and flavonoids increased with the order of pre-flowering, full-flowering and post-flowering. Dailyharvesting time (diurnal variability) caused no effect on antioxidant activity, total phenols and flavonoids. Inconclusion, antioxidant activity of bitter fennel extracts significantly differs according to plant organs andgrowing stages.
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- Aazza S, Lyoussi B, Megías C, Cortés-Giraldo I, Vioque J, Figueiredo AC, Miguel MG. (2014). Anti-oxidant,
anti-inflammatory and anti-proliferative activities of Moroccan commercial essential oils. Natural
Product Communications. 9 (4): 587-594.
- Açıkgöz MA, Kara ŞM, Aruç C, Ay E (2017a). Morphogenetic, ontogenetic and diurnal variability in content
and constituents of bitter fennel (Foeniculum vulgare Miller var. vulgare) essential oil. International
Conference on Agriculture Forest Food, 15-17 May, 2017, Cappadocia, Turkey.
- Açıkgöz MA, Kara ŞM, Aruç C, Ay E (2017b). Morphogenetic, ontogenetic and diurnal variability in
antimicrobial activity of bitter fennel (Foeniculum vulgare Miller var. vulgare) essential oil. Indian J of
Pharmaceutical Education and Research. 51 (3): 190-194.
- Anwar F, Ali M, Hussain AI, Shahid M (2009a). Antioxidant and antimicrobial activities of essential oil and
extracts of fennel (Foeniculum vulgare Mill.) seeds from Pakistan. Flavour and Fragrance Journal. 24
(4): 170-176.
- Anwar F, Hussain AI, Sherazi STH, Bhanger MI (2009b). Changes in composition and antioxidant and
antimicrobial activities of essential oil of fennel (Foeniculum vulgare Mill.) fruit at different stages of
maturity. Journal of Herbs, Spices and Medicinal Plants.15 (2):187–202.
- Badgujar SB, Patel VV, Bandivdekar AH (2014). Foeniculum vulgare Mill: A review of its botany,
phytochemistry, pharmacology, contemporary application, and toxicology. BioMed Research
International. 2014: 01-32.
- Bakkali F, Averbeck S, Averbeck D, Idaomar M (2008). Biological effects of essential oils – A review. Food
and Chemical Toxicology. 46 (2): 446-475.
- Barros L, Heleno SA, Carvalho AM, Ferreira IC (2009). Systematic evaluation of the antioxidant potential of
different parts of Foeniculum vulgare Mill. from Portugal. Food and Chemical Toxicology. 47 (10):
2458-2464.
- Biesalski HK, Dragsted LO, Elmadfa I, Grossklaus R, Müller M, Schrenk D, Walter P, Weber P (2009).
Bioactive compounds: Definition and assessment of activity. Nutrition. 25 (11-12): 1202-1205.
- Dalar A, Türker M, Konczak I (2012). Antioxidant capacity and phenolic constituents of Malva neglecta Wallr.
and Plantago lanceolata L. from Eastern Anatolia Region of Turkey. Journal of Herbal Medicine. 2 (2):
42-51.
- Diao WR, Hu QP, Zhang H, Xu JG (2014). Chemical composition, antibacterial activity and mechanism of action
of essential oil from seeds of fennel (Foeniculum vulgare Mill.). Food Control. 35 (1): 109-16.
- Dua A, Garg G, Mahajan R (2013). Polyphenols, flavonoids and antimicrobial properties of methanolic extract
of fennel (Foeniculum vulgare Miller). European Journal of Experimental Biology. 3 (4): 203-208.
- Faudale M, Viladomat F, Bastida J, Poli F, Codina C (2008). Antioxidant activity and phenolic composition of
wild, edible, and medicinal fennel from different Mediterranean countries. J. Agric. Food Chem. 56
(6): 1912-1920.
- Figueiredo AC, Barroso JG, Pedro LG, Scheffer JJC (2008). Factors affecting secondary metabolite production
in plants: volatile components and essential oils. Flavour Fragr. J. 23: 213–226.
- Hussain AI, Anwar F, Sherazi STH, Przybylski R (2007). Chemical composition, antioxidant and antimicrobial
activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food
Chemistry. 108 (3): 986-995.
- Miguel MG, Cruz C, Faleiro L, Simoes MT, Figueiredo AC, Barroso JG (2010). Foeniculum vulgare essential
oils: chemical composition, antioxidant and antimicrobial activities. Natural Product Communications.
5 (2): 319-328.
- Oktay M, İ. Gülcin, Küfrevioglu ÖI (2003). Determination of in vitro antioxidant activity of fennel (Foeniculum
vulgare) seed extracts. Food Science and Technology. 36 (2): 263-271.
- Özcan MM, Chalchat JC, Arslan D, Ateş A, Ünver A (2006). Comparative essential oil composition and
antifungal effect of bitter fennel (Foeniculum vulgare ssp. piperitum) fruit oils obtained during different
vegetation. Journal of Medicinal Food. 9 (4): 552-561.
- Park YS, Kim SJ, Chang HI (2008). Isolation of anthocyanin from black rice (Heugjinjubyeo) and screening of
its antioxidant activities. Kor. J. Microbiol. Biotechnol. 36 (1):55-60.
- Roby MHH, Sarhan MA, Selim KAH, Khalel KI (2013). Antioxidant and antimicrobial activities of essential oil
and extracts of fennel (Foeniculum vulgare L.) and chamomile (Matricaria chamomilla L). Industrial
Crops and Products. 44: 437-45.
- Salama ZA, Baz FKE, Gaafar AA, Zaki MF (2015). Antioxidant activities phenolics, flavonoids and vitamin C
in two cultivars of fennel (Foeniculum vulgare Mill.) in response to organic and bio-fertilizers. Journal
of the Saudi Society of Agricultural Sciences. 14: 91-99.
- Shahat AA, Ibrahim AY, Hendawy SF, Omer EA, Hammouda FM, Abdel-Rahman FH (2011). Chemical
composition, antimicrobial and antioxidant activities of essential oils from organically cultivated fennel
cultivars. Molecules. 16 (2): 1366-1377.
- Shimada K, Fujikawa K, Yahara K, Nakamura T (1992). Antioxidative properties of xanthone on the auto
oxidation of soybean in cylcodextrin emulsion. J. Agr. Food Chem. 40 (6): 945-8.
- Teixeria B, Marquesa A, Ramos C, Neng NR, Nogueira JMF, Saraiva JA, Nunes MA (2013). Chemical
composition and antibacterial and antioxidant properties of commercial essential oils. Industrial Crops
and Products. 43: 587-595.
- Telci I, Demirtas I, Sahin A (2009). Variation in plant properties and essential oil composition of sweet fennel
(Foeniculum vulgare Mill.) fruits during stages of maturity. Industrial Crops and Products. 30 (1): 126-
30.
- Wolfe K, Wu X, Liu RH (2003). Antioxidant activity of apple peels. J. Agric. Food Chem. 51: 609-614.
- Zujko ME, Witkowska AM (2011). Antioxidant potential and polyphenol content of selected food. International
Journal of Food Properties. 14: 300–308.