Bülent ŞENGÜL, Yeliz DEMİR, Şükrü ÖNALAN

Gökkuşağı Alabalığı (Oncorhynchus mykiss) İşletmelerinde Bazı Antioksidan Enzim Aktivitelerinin Ağır Metal İnhibisyonlarının Araştırılması

Endüstriyel, tarımsal ve ticari kimyasalların, sucul ekosistemlerde meydana getirdiği kirlilik, giderek artanciddi bir problemdir. Çevresel kirleticiler, sucul organizmalarda çeşitli zararlı etkilere neden olur. Balıklar, suculorganizmalar arasında ağır metallere en çok maruz kalan türlerdir. Ağır metallerin balıklarda birikmesi balıkhastalıkları konusunda önemli bir sorun olmanın yanında, oksidatif stres sebebi olan reaktif oksijen türlerinin(ROT) artmasına da neden olmaktadır. Biyolojik sistemlerde oksidatif strese bağlı reaktif oksijen türlerindekiartış antioksidan savunma sistemlerinde hasara yol açar. Bu çalışmanın amacı gökkuşağı alabalık çiftliklerindeüretimi yapılan balıkların karaciğer, böbrek ve solungaç dokularındaki bazı antioksidan enzim aktiviteleri ileağır metal iyonlarından kaynaklanan su kirliliği arasındaki ilişkiyi ortaya koymaktır. Bu amaçla; Suörneklerindeki ağır metal iyonlarının (Zn, Fe, Cu, Ni, Co, Hg, As, Mn) seviyeleri belirlenerek, bu iyonlarınbalıklardan alınan kalp, karaciğer ve solungaç dokularındaki antioksidan enzimlerin (Süperoksid dismutaz(SOD), Katalaz (CAT), Glutatyon redüktaz (GR), Glutatyon S-transferaz (GST), Glutatyon peroksidaz (GPx))aktiviteleri üzerine etkileri incelenmiştir.

Investigation of Heavy Metal Inhibitions of Some Antioxidant Enzyme Activities in Rainbow Trout (Oncorhynchus mykiss) Farms

The increasing pollution of industrial, agricultural and commercial chemicals in aquatic ecosystems is an increasingly serious problem. Environmental pollutants cause a variety of harmful effects in aquatic organisms. Fish are the species most exposed to heavy metals among aquatic organisms. Accumulation of heavy metals in fish is a major problem for fish diseases as well as an increase in reactive oxygen species (ROT), which is the cause of oxidative stress. The increase in oxidative stress-related reactive oxygen species in biological systems leads to damage in antioxidant defense systems. The purpose of this study is to establish the relationship between some antioxidant enzyme activities in the liver, kidney and gill tissues of fish produced in rainbow trout farms and the water pollution caused by heavy metal ions. For this purpose; The levels of heavy metal ions (Zn, Fe, Cu, Ni, Co, Hg, As, Mn) in water samples were determined and these antioxidant enzymes (Superoxide dismutase (SOD), Catalase (CAT), Glutathione reductase (GR), glutathione S-transferase (GST), glutathione peroxidase (GPx)) activities.

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