Süleyman ÖZAKIN, Bilgin TAŞKIN, Ebru İNCE BOSTANCI

Lokal Endemik Bitki Rizosferinden İzole Edilen Aktinobakterilerin Tanımlanması ve Antimikrobiyal Aktivitesi

Patojenlerin direncinin artmasıyla birlikte yeni antimikrobiyal bileşik arayışı hız kazanmaktadır. Bu çalışmada, Türkiye'deki üç yerel endemik bitkinin rizosfer topraklarından izole edilen 11 lokal Aktinobakteri izolatının farklı patojen bakterilere ve Candida albicans'a karşı antimikrobiyal potansiyeli değerlendirilmiştir. In vitro antimikrobiyal aktivite, modifiye Bennett's ve Tryptic Soy Broth (TSB) besiyerinde üretilen izolatların organik özütleri kullanılarak disk difüzyon agar yöntemiyle araştırılmıştır. Sekiz izolatın patojenlere karşı kayda değer antimikrobiyal aktiviteye sahip olduğu belirlenmiştir. Biyoaktif metabolitlerin üretimi üzerine iki farklı kültür ortamının incelenmesi sonucu, modifiye Bennett ortamında daha yüksek antimikrobiyal aktivitenin elde edildiği bulunmuştur. Ayrıca kısmi 16S rDNA dizi analizleri ile bu lokal suşların 10'unun Streptomyces ve birinin de Promicromonospora cinsine ait olduğu tespit edilmiştir.

Anahtar Kelimeler:

İkincil Metabolitler, 16S rRNA, Aktinobakteriler, Antimikrobiyal aktivite, Moleküler tanımlama

Identification and Antimicrobial Activity of Actinobacteria Isolated from Rhizosphere of the Local Endemic Plants

The search for novel antimicrobial compounds from the environment has been gaining momentum with the increase in resistance of pathogens. In the present study, the antimicrobial potential of 11 local isolates of Actinobacteria which were isolated from rhizosphere soils of the three local endemic plants in Turkey was evaluated against different pathogenic bacteria and Candida albicans. Antimicrobial activity was investigated by the disk diffusion agar method using the organic extracts obtained from the isolates grown in the modified Bennett's medium and Tryptic Soy Broth (TSB). Eight isolates were revealed to show remarkable antimicrobial activity against to pathogens. Study of the production of bioactive metabolites in two different culture media indicated that the higher antimicrobial activity was observed in modified Bennett's medium when compared to TSB. Also, partial 16S rDNA sequence analyses revealed that 10 of these local isolates belong to member of Streptomyces and one of them to member Promicromonospora genera.

Keywords:

Secondary Metabolites 16S rRNA, 16S rRNA, Actinobacteria, Antimicrobial activity, Molecular identification,

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