Genetic relationships among various sihke melon landraces

Yerel ve eski çeşitler, modern tarımda yok olma tehdidi ile karşı karşıya bulunmaktadır. Bu nedenle, yerel ve eski çeşitler mevcut çeşitlerin sahip olmadığı genleri içerebileceğinden dolayı, çeşitliliğin korunması ıslah çalışmaları için hayati önem taşımaktadır. Mevcut çalışma, Sıhke kavun yerel çeşidi arasındaki genetik ilişkileri fenotipik özellikler ile belirlemek amacıyla yürütülmüştür. Çalışmanın ana materyalini, Türkiye’nin Van ilinde çeşitli yerlerden toplanmış 15 Sıhke Kavun genotipi oluşturmuştur. Ayrıca, 2 yabancı standart kavun genotipi (Sembol F1 ve Sempati F1) ve daha önce karakterize edilmemiş 13 yerel kavun genotipi, fenotipik karakterizasyona dahil edilmiştir. Fenotipik karakterizasyonda kullanılmak üzere, kavun genotiplerine ait toplam 63 adet ölçüm veya gözlemden yararlanılmıştır. Kavun genotipleri arasındaki genetik akrabalık dereceleri, fenotipik veriler kullanılarak elde edilen Öklid matrislerinden dendrogramlar, 2 ve 3 boyutlu ölçeklemeler oluşturularak incelenmiştir. Bu çalışma ile, Van yöresi Sıhke kavun yerel çeşidi ve bazı yabancı ve yerli genotiplerin fenotipik özellikleri belirlenmiştir. Bununla birlikte Sıhke yerel kavun çeşidinin kendi içerisindeki ve diğer yerli ve yabancı genotiplerle aralarındaki genetik ilişkiler ortaya çıkartılmıştır. Moleküler Öklid matrisine göre, Sıhke yerel kavun çeşidi, diğer genotiplerden farklı çıkmış ve kendi aralarında grup oluşturma eğilim göstermiştir. Çalışılan genotipler içerisinde, Genotip 30-Şemame en farklı genotip olarak bulunmuştur. Geri kalan genotipler iki ana gruba ayrılmışlardır.

Farklı Sihke yerel kavun genotipleri arasındaki genetik ilişkiler

Landraces and older varieties face with extinction in modern agriculture. Therefore, the preservation of diversity becomes vital for future breeding efforts because landraces and older varieties might contain genes that current varieties do not. The present study aimed to determine genetic relationships among various Sihke melon landraces for phenotypic traits. The main materials of the study were 15 Sihke melon genotypes collected from various parts at Van province of Turkey. Moreover, 2 foreign standard melon genotypes (Sembol F1 and Sempati F1) and 13 local melon genotypes that previously have not been characterized were included into the phenotypic characterization. Total 63 measurements or observations were used to define the genetic similarity among the studied melon genotypes by dendrograms or two- and three-dimensional scaling obtained from Euclidian distance matrix. With this study, the phenotypic traits of Sihke melon landrace of Van and some other local and foreign melon genotypes were determined. Moreover, the genetic relatedness among Sihke melon populations and other local and foreign genotypes were examined. Based on the molecular Euclidean distance matrix, Sihke melon landraces were different from the others and tended to be grouped together. Among the studied genotypes, the most distinct genotype was Genotype 30 called Semame. The remaining genotypes were divided into three main groups.

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Acampora A, Ciaffi M, Pace DP, Paolacci AR, Tanzarella OA (2007). Pattern of variation for seed size traits and molecular markers in Italian germplasm of Phaseolus coccineus L.. Euphytica. 157:69–82.
Anonymous (2010). FAOSTAT. Statistic Database. http://faostat.fao.org/ Günay A (1993). Vegetable Production V. A.Ü. Ziraat Fak. Ankara. 117 s. (in Turkish).
Jeffrey C (2001). Cucurbitacae. In: Hanelt P. and Institute of Plant Genetics and Crop Plant Research (eds.), Mansfeld’s Encyclopedia of Agricultural and Horticultural Crops. Springer, New York, NY, USA.
Küçük A, Abak K, Sarı N (2002). Cucurbit genetic resources collections in Turkey. First AD HOC Meeting on Cucurbit Genetic Resources. 19 January 2002, Adana, Turkey. 46-51.
Laghetti G, Accogli R, Hammer K (2008). Different cucumber melon (Cucumis melo L.) races cultivated in Salento (Italy). Genet. Resour. Crop. Evol. 55(4):619-623.
Luan F, Delannay I, Staub JE (2008). Chinese melon (Cucumis melo L.) diversity analyses provide strategies for germplasm curation, genetic improvement, and evidentiary support of domestication patterns. Euphytica 164, 445–461.
Piergiovanni AR, Cerbino D, Brandi (M) (2000). The common bean populations from Basilicata (Southern Italy). An evaluation of their variation. Genet. Resour. Crop. Evol. 47: 489–495.
Pitrat M, Chauvet M, Foury C (1999). Diversity, history and production of cultivated cucurbits. Proc. 1st Int. Symp. on Cucurbits. (Eds. K. Abak & S. Büyükalaca.) Acta. Hort. 492:21-28.
Rabbani MA, Murakami Y, Kuginuki Y, Takayanagi K (1998a). Genetic variation in radish (Raphanus sativus L.) germplasm from Pakistan using morphological traits and RAPDs. Genet. Resour. Crop. Evol. 45: 307–316.
Rabbani MA, Iwabuchi A, Murakami Y, Suzuki T, Takayanagi K (1998b). Phenotypic variation and the relationships among mustard (Brassica juncea L.) germplasm from Pakistan. Euphytica. 101: 357–366.
Robinson RW, Decker-Walters DS (1997). Cucurbits. CAB Int. University Press, Cambridge. 226 s.
Sari N, Tan A, Yanmaz R, Yetisir H, Balkaya A, Solmaz I, Aykas L (2008). General status of cucurbit genetic resources in Turkey. Pitrat M. (ed): Cucurbitaceae 2008, Proceedings of the IXth EUCARPIA meeting on genetics and breeding of Cucurbitaceae, Avignon (France), May 21-24th, 2008, pp. 21-32
Sensoy S, Buyukalaca S, Abak K (2007a). Evaluation of genetic diversity in Turkish melons (Cucumis melo L.) based on phenotypic characters and RAPD markers. Genet. Resour. Crop. Evol. 54:1351-1365.
Sensoy S, Demir S, Buyukalaca S, Abak K (2007b). Inoculation tests and RAPD markers determine response of Turkish melon genotypes to Fusarium oxysporum f.sp. melonis Race 1. Eur. J. Hort. Sci. 72(5):220-227
Silberstein L, Kovalski I, Huang R, Anagnostou K, Jahn MMK, Perl-Treves R (1999). Molecular variation in melon (Cucumis melo L.) as revealed by RFLP and RAPD markers. Scientia Horticulturae. 79:101-111.
Staub JE, Box J, Meglic V, Horejsi T, Mc Creight JD (1997). Comparison of isozyme and random amplified polymorphic DNA data for determining intraspecific variation in Cucumis. Genet. Resour. Crop. Evol. 44:257-269.
Stepansky A, Kovalski I, Perl-Treves R (1999). Intraspecific classification of melons (Cucumis melo L.) in view of their phenotypic and molecular variation. Plant. Syst. Evol. 217:313-332.
Szamosi C, Solmaz I, Sari N, Bársony C (2010). Morphological evaluation and comparison of Hungarian and Turkish melon (Cucumis melo L.) germplasm. Scientia Horticulturae. 124(2):170-172.
Türkmen Ö, Şensoy S, Demir S, Yıldız M (2005). Improvement in Local Lake Van Basin Melon Populations and Comparison of the Selected Genotypes with Commercial Cultivars. TUBITAK-TOGTAG Project No:2681. (in Turkish).
Yetişir H, Şakar M, Serçe S (2008). Collection and morphological characterization of Lagenaria siceraria germplasm from the Mediterranean region of Turkey. Genet. Resour. Crop. Evol. 55(8):1257-1266.
Yildiz M, Ekbic E, Keles D, Sensoy S, Abak K (2011). Use of ISSR, SRAP, and RAPD markers to assess genetic diversity in Turkish melons. Scientia Horticulturae. 130 (2011) 349–353.
Zhukovsky P (1951). Agricultural Structure of Turkey (Anatolia). Türkiye Şeker Fab. AŞ. 1951. Yay.No:20. 887 s. (in Turkish).