Astragalus Gummifer (Günizer)’in Toprak Üstü Kısımlarının Liyofilize Su Ekstresi Antioksidan Aktivitesi

Araştırmalar, koroner kalp hastalığı ve kanser gibi yaşa bağlı hastalıklar ile meyve ve sebze tüketimi arasında ters bir korelasyon olduğunu göstermiştir. Bitkilerden elde edilen doğal antioksidanların olası sağlık yararları dikkat çekmekte ve bunlarla ilgili araştırmalar artmaktadır. Fabaceae familyasının en büyük cinsi Astragalus L.’dir ve Türkiye florasında 224 endemik türünün varlığı tespit edilmiştir. Astragalus türleri Türkiye’nin birçok bölgesinde yetişen ve geleneksel tıpta kullanılan bitkiler olduğu için bu türlerin biyolojik aktivitelerinin değerlendirilmesi bilimsel açıdan önem taşımaktadır. Bu çalışmada Astragalus gummifer’in antioksidan özellikleri araştırıldı. Toprak üstü kısımlarından elde edilen liyofilize su ekstresinin antioksidan kapasitesi ferrik iyonlar (Fe3+) indirgeme, KUPRAK, FRAP, DPPH• ve ABTS•+ giderici antioksidan yöntemleri ile değerlendirildi. Astragalus gummifer’in ABTS•+ giderme aktivitesi ölçüldü ve IC50 değeri 10.04 μg/mL olarak bulundu. Sonuçlar Astragalus gummifer’in ABTS•+ giderme aktivitesinin α-tokoferole (8.058 μg/mL) yakın olduğunu gösterdi. Bu araştırma, Astragalus gummifer’in konu olacağı daha ileri fitokimyasal araştırmaları için bir temel oluşturmaktadır.

Anahtar Kelimeler:

antioksidan aktivite, Astragalus gummifer, İndirgeme gücü, Radikal giderme, Total fenol, Total flavonoit

Antioxidant Activity of Lyophilized Water Extract of Aerial Parts of Astragalus Gummifer (Günizer)

Studies have shown an inverse correlation between age-related illnesses such as coronary heart disease and cancer, and fruit and vegetable consumption. Given the possible health benefits of natural antioxidants from plants, the research on the subjects has increased. 224 endemic species of Astragalus L., the largest genus of the Fabaceae family, were identified in the flora of Turkey. Since Astragalus species are plants grown in many regions of Turkey and used in traditional medicine, it is scientifically important to evaluate the biological activities of these species. In this study, we investigated the antioxidant properties of Astragalus gummifer. The antioxidant capacity of the lyophilized water extract obtained from the aerial parts was evaluated by ferric ions (Fe3+) reduction, KUPRAK, FRAP, DPPH• and ABTS+• scavenging antioxidant methods. ABTS•+ scavenging activity of Astragalus gummifer was measured and IC50 value was found to be 10.04 μg/mL. The results showed that ABTS•+ scavenging activity of Astragalus gummifer is close to α-tocopherol (8.058 μg/mL). This research provides a basis for further phytochemical studies on Astragalus gummifer.

Keywords:

Antioxidant activity, Astragalus gummifer, Radical scavenging, Reducing power, Total flavonoid, Total phenol,

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