Yusuf BİÇER, Gonca SÖNMEZ, Gamze TURKAL, Tahir YILMAZ, Muhammed Hüdai ÇULHA, Gürkan UÇAR

Koyun ve keçi sütlerinde inek sütünün TaqMan Real-Time PCR ile tespit edilmesi

Süt ve süt ürünleri içerdikleri yüksek besin değeriyle günlük diyetin önemli bir parçasını oluşturmaktadır. Ancak bu önemli özelliklerinin yanı sıra en fazla hile yapılan gıdalar arasında yer almaktadır. Koyun-keçi sütü ve ürünlerine inek sütünün karıştırılması süt ve süt ürünlerinde en sık karşılaşılan hilelerin başında gelmektedir. Bu durum, tüketiciler tarafından tercih edilmeyen sosyo-ekonomik potansiyel risklere neden olmaktadır. Bu çalışmada koyun ve keçi sütlerine farklı oranlarda karıştırılan inek sütü miktarının TaqMan Real-Time PCR ile tespit edilmesi amaçlanmıştır. Bu amaçla %1, %5, %10, %25, %75 ve %90 oranlarında keçi ve koyun sütlerine inek sütü karıştırılmıştır. Ayrıca saf inek sütünden elde edilen DNA sulandırılarak PCR işleminin duyarlılığı araştırılmıştır. Bu çalışmanın sonucunda, koyun ve keçi sütlerine karıştırılan %1 inek sütü ve 0,003 ng DNA varlığı tespit edilmiştir. Araştırma sonucunda TaqMan Real-Time PCR’ın koyun ve keçi sütlerine karıştırılan düşük düzeydeki inek sütünün tespit edilmesinde güvenilir ve hassas bir yöntem olarak kullanılabileceği düşünülmektedir.

Anahtar Kelimeler:

inek sütü, keçi sütü, koyun sütü, TaqMan Real-Time PCR, tür tespiti

Detection of cow milk in sheep and goat milk by TaqMan Real-Time PCR

Milk and dairy products constitute an important part of the daily diet due to their high nutritional value. However, in addition to these important features, it is among the most adulterated foods. The most common adulteration in milk and dairy products is the mixing of cow's milk with sheep and goat milk and products. This situation causes socio-economic potential risks that are not preferred by consumers. In this study, it was aimed to determine the amount of cow's milk mixed with sheep and goat milk at different rates by TaqMan Real-Time PCR. For this purpose, 1%, 5%, 10%, 25%, 75% and 90% cow's milk was mixed with goat and sheep milk. In addition, the sensitivity of the PCR process was investigated by diluting the DNA obtained from pure cow's milk. As a result of this study, the presence of 1% cow's milk and 0.003 ng DNA mixed with sheep and goat milk was determined. As a result of the research, it is thought that TaqMan Real-Time PCR can be used as a reliable and sensitive method for detecting low level cow's milk mixed with sheep and goat milk.

Keywords:

cow milk, goat milk, sheep milk, TaqMan Real-Time PCR, authentication,

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