Epididimal manda spermasının dondurulmasında spermaya katılan farklı sulandırıcıların spermatolojik parametreler üzerine etkisinin in-vitro değerlendirilmesi

Yapılan bu araştırmanın amacı, mezbahanelerden kesim sonrası alınan manda testislerinden elde edilen epididimal spermalarda çeşitli sperma sulandırıcıları kullanarak dondurmanın bazı spermatolojik parametreler üzerine etkisinin incelenmesidir. Mezbahanelerden kesim sonrası alınan 15 adet erkek Anadolu mandası (3 yaş ve üzeri) testislerinden elde edilen epididimal spermalara 6 farklı sperma sulandırıcısı (Tris, OptixCell®, BioXcell®, BullXcell®, AndroMed®, Steridly®) katılarak yapılan spermatolojik muayenelerle spermatozoonların motilitesi, ölü/canlı spermatozoon oranı, anormal spermatozoon oranı ve Hipo Osmotik Şişme Testi (HOST) oranları saptandı. Araştırmada taze sperma ile çözüm sonu sperma motilitesi, ölü spermatozoon oranı, HOST oranı, orta kısım, kuyruk ve toplam anormal spermatozoa oranları arasında önemli (p

Anahtar Kelimeler:

Dondurma, Epididimal, Manda, Sperma, Sulandırıcı.

In-vitro evaluation of the effects of different extenders on spermatological parameters in the freezing of epididymal buffalo sperm

The aim of this study is to examine the effect of freezing on some spermatological parameters by using various semen extenders in epididymal semen obtained from buffalo testicles taken from slaughterhouses after slaughter. Six different semen extenders (Tris, OptixCell®, BioXcell®, BullXcell®, AndroMed®, Steridly®) were added to the epididymal semen obtained from 15 male Anatolian buffalo testicles taken from slaughterhouses after slaughter and evaluated by performing spermatological examinations. In the study, it was determined that there were significant (p0.05). Considering these parameters in our study, it is seen that the Tris extender is the semen extender that gives the best results. As a result, it is thought that epididymal buffalo semen obtained post-mortem can be evaluated using biotechnological methods.

Keywords:

Buffalo, Epididymal, Extender, Freezing, Sperm.,

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