Uzun Transporta Bağlı Aygırların Strese Tepkileri: Tam Kan Sayımı ve Serum Biyokimya Varyasyonları
Bu çalışmada, transportun atlarda
bazı stres göstergelerine etkisini belirlemek amaçlanmıştır. 7 Safkan, 2 Arap,
1 Hollanda Yarım Kan ve 1 Fresyan olmak üzere 3-12 yaş arası 11 olgun at
(aygır) kayıt edildi. Transport öncesi
ve sonrasında, otomatik kan sayımı ile beyaz kan hücresi sayımı ve diğer ilgili
parametreler belirlendi. İlgili biyolojik belirteçlerin serum aktiviteleri bir
otoanalizör kullanılarak belirlendi. Na, K, Cl ve Ca düzeyleri de otoanalizör
kullanılarak ölçüldü. Ortalama Platelet (PLT) ve Oratalama Hemoglobin
Konsantrasyonu (MCHC) Total Lökosit (WBC) sayıları çalışma süresince sağlıklı
atlar için bir farklılık göstermezken, Alyuvarlar (RBC), Hemoglobin (Hb) ve
Hematokrit (Hct) düzeyleri ulaşımdan sonra anlamlı artışlar sergiledi (P<0.05).
Günlük ortalama glikoz konsantrasyonu transporttan sonra artış gösterdi (P<0.05).
Ortalama total protein, albümin, Aspartat aminotransferaz (AST) ve Kreatinin
kinaz (CK) düzeyleri, transportun ardından 2. günde artmaktadır (P<0.05).
Ortalama kan üresi azotu, kreatinin, total bilirubin, Alanin aminotransferaz
(ALT), Alkalen fosfataz (ALP), Laktat dehidrogenaz (LDH) ve Gama-gulutamil
Transferaz (GGT), çalışma boyunca önemli değişiklikler göstermedi. Transporttan
sonra ortalama Sodyum (Na), Potasyum (K) ve Klor (Cl) düzeyleri değişti (P<0.05),
nakil sonrası Kalsiyum (Ca) düzeyleri anlamlı düşüş gösterdi (P <0.05).
Anahtar Kelimeler:
Aygır, Biyokimya, Hematoloji, Stres, Transport
Stress Responses of Stallions During Transport Period: The Variations of Complete Blood Count and Serum Biochemistry
In the present study the objective was to determine the effect of one
transport journey on some stress indicators in horses. 11 adult stallion (all
stallion) age 3-12 years including 7 Thoroughbred, 2 Arabian, 1 Dutch
Warmblood, and 1 Friesian were enrolled. White blood cell counts and other
relevant parameters were determined by an automated cell counter before and
after transportation. Serum activities of relevant biomarkers were determined
by use of an autoanalyzer. Serum cortisole level was detected by a microplate
immunoassay technique. Sodium (Na), Potassium (K) and Clor (Cl) and Calsium
(Ca) levels were also measured by use of autoanalyser. Mean Platelet (PLT), and
Mean corpuscular hemoglobin concentration
(MCHC) White Blood Cell (WBC) counts did not exhibit a variation for
healthy horses during the study whereas RBC, Hb and Hct levels exhibited
significant increases after transportation (P<0.05). Mean daily glucose
concentration showed an increase after transportation (P< 0.05). Mean total
protein, albumine, AST and CK levels presented increases on day 2, after
transportation (P< 0.05). Mean blood urea nitrogen, creatinine, total
bilirubine, ALT, ALP, LDH and GGT did not show significant changes throughout
the study. Mean Sodium (Na), Potassium (K) and Clor (Cl) levels showed
alterations after transportation (P< 0.05), whereas Calsium (Ca) levels
showed significant decreases after transportation (P< 0.05).
Keywords:
Biochemistry, Hematology, Stallion, Stress, Transport,
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- Başlangıç: 2022
- Yayıncı: Atatürk Üniversitesi