İzole Edilmiş Rat Mesane Düz Kas Kontraksiyonları Üzerine Rosmarinus officinalis (Rosemary) Aköz Ekstraktının Etkileri
Rosmarinus officinalis (Rosemary) geleneksel tıpta
yaygın olarak kullanılmaktadır ve o diüretik etkiye ve ileum, trakea, mide düz
kasları üzerine gevşetici etkilere sahiptir. Fakat Rosemary’nin idrar kesesi
düz kaslarının miyojenik aktiviteleri üzerine herhangi bir çalışmaya
rastlanılmamıştır. Bu çalışmada izole edilmiş rat mesane düz kas
kontraktilitesi üzerine Rosemary ekstraktının etkileri araştırılmıştır. Kas
şeritleri Sprague Dawley cinsi erkek ratların mesanesinden hazırlandı.
Hazırlanan kas şeritleri Krebs solüsyonu içeren izole organ banyosuna
yerleştirildi. Kas şeritler ACh (10-4 M) ve KCl (60 mM) ile
uyarılarak kastırıldı ve bu değerler referans değer olarak kabul edildi. ACh ve
KCl ile indüklenen kontraksiyonlar üzerine Rosemary ekstraktının farklı
dozlarının (150, 300, 600 ve 1200 µg/mL ) etkileri belirlendi. Banyoda Rosemary’nin
dozlarının varlığında ACh ve KCl ile indüklenen kontraksiyonların referans
değerlerden farklılık arz etmediği belirlendi (P>0.05). Mevcut çalışmada, Rosemary
ekstraktının çeşitli dozlarının rat idrar kesesi düz kaslarının myojenik
aktivitesi üzerine etkisinin olmadığı görüldü. Çeşitli amaçlar için Rosemary
kullanımının idrar kesesi düz kaslarının fizyolojik aktivitesi üzerine
istenmeyen etkilerinin olmadığı düşünülebilir.
Anahtar Kelimeler:
Düz kas, İdrar kesesi, Rat, Rosmarinus officinalis, Sulu ekstrakt
The Effects of Rosmarinus officinalis (Rosemary) Aqueous Extract on Smooth Muscle Contractions in Isolated Rat Urinary Bladder
Rosmarinus officinalis (Rosemary) is widely used in traditional
medicine and it has diuretic and spasmolytic effects on the ileum, trachea, and
stomach smooth-muscle. However, a study the related effects of Rosemary on the
myogenic activities of urinary bladder smooth-muscles has not been founded. The present study investigated the effects of
Rosemary extract on the smooth-muscle contractility of isolated rat urinary
bladder. Muscle strips were prepared from urinary bladder of male Sprague
Dawley rats. Prepared muscle strips were placed in an isolated organ bath
containing Krebs solution. The muscle strips were pre-contracted by inducing with
Ach (10-4M) and KCl (60mM), and these values were considered to be
reference values. The effects of the different doses (150, 300, 600 and 1200µg/mL)
of Rosemary extract on ACh and KCl-induced contractions were determined. It was
determined that no different from references values of the contractions induced
by ACh and KCl in the presence of Rosemary doses in bath (P>0.05). In the present study, the various doses of Rosemary extract had no
effect on myogenic activity in rat urinary bladder smooth-muscle. It may be
conceive that the use of Rosemary for different purposes does not result in an
undesirable effect on the physiological activities of bladder smooth-muscle.
Keywords:
Aqueous extract, Urinary bladder, Rat, Rosmarinus officinalis, Smooth muscle,
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- Başlangıç: 2022
- Yayıncı: Atatürk Üniversitesi