Ruhi KABAKÇI, Yasemin ÇINAR, Miyase ÇINAR

Sıçanlardaki Tiyol-Disülfit Dengesi Üzerine Ginkgo Biloba Özü ve Akut Yorucu Egzersizin Birlikte Uygulanmasının Etkileri Nelerdir?

Bu çalışma Ginkgo Biloba özü (EGB761)’nün ve akut yorucu egzersizin tek ve birlikte uygulanmasının ratlardaki tiyol-disülfit homeostazı (TDH) üzerine etkilerini değerlendirmeyi amaçladı. Toplam 32 sıçan ile 4 deneysel grup oluşturuldu. Kontrol (C) ve egzersiz (E) gruplarındaki hayvanlara, 4 hafta boyunca haftada 5 gün 2 mL/kg %0.9 salin solüsyonu verilirken, Ginkgo Biloba (GB) ve Ginkgo Biloba artı Egzersiz (GB + E) gruplarındaki diğerlerine aynı süre zarfında 100 mg/kg EGB761 verildi. Bu dönemin sonunda, E ve GB+E gruplarındaki sıçanlar, %5 eğimde 25 m/dk hızda yoruluncaya kadar koşturuldu. Tüm plazma örnekleri malondyaldehit (MDA) ve nitrik oksit (NO) seviyeleri ve süperoksit dismutaz (SOD) aktivitesi ve TDH için değerlendirildi. Çalışmanın bulguları, egzersizin GB+E grubunda MDA seviyelerini (P < ,001) ve SOD aktivitesini (P < ,05) önemli ölçüde arttırırken, GB grubunda yalnızca MDA düzeylerinin önemli oranda azaldığını (P < ,05) göstermiştir. Öte yandan, ne egzersiz ne de EGB761 uygulaması NO seviyesini ve TDH ile ilgili parametreleri etkilemiştir (P > ,05). EGB761, MDA’yı azaltarak oksidatif stresi önleyebilse de, egzersizdeki kullanımının, özellikle yeni bir oksidatif stres belirteci olan TDH açısından ilerde incelenmesi gerekmektir.

Anahtar Kelimeler:

Akut egzersiz, ginkgo biloba, oksidatif stres, tiyol-disülfür homeostaz, Acute exercise, oxidative stress, thiol-disulfide homeostasis

What is the Combined Effects of Ginkgo Biloba Extract (EGb761) and Acute Exhaustive Exercise on the Thiol-Disulfide Homeostasis in Rats?

This study aimed to evaluate the alone and combined effects of ginkgo biloba extract (EGb761) and acute exhaustive exercise on thiol-disulfide homeostasis (TDH) in male rats. Four experimental groups were formed with total 32 rats. Animals in the control and exercise groups received 2 mL/kg of 0.9% saline solution 5 days a week for 4 weeks, while the others in the ginkgo biloba and ginkgo biloba plus exercise groups received 100 mg/kg of EGb761 for the same duration. At the end of this period, rats in groups E and GB+E were made to run on a treadmill at 25 m/min at 5% incline until exhaustion. All plasma samples were evaluated for malondialdehyde (MDA) and nitric oxide (NO) levels, superoxide dismutase activity (SOD), and TDH. The findings of the study showed that exercise significantly increased MDA levels (P < .001) and SOD activity (P < .05) in group GB+E, while only MDA levels were decreased significantly (P < .05) in group GB. On the other hand, neither exercise nor EGb761 could affect the NO levels and the parameters related to TDH (P > .05). Although EGb761 can prevent oxidative stress by reducing MDA, its usage in exercise still needs to be further investigated, especially in terms of TDH that is a new important oxidative stress marker.

Keywords:

Acute exercise, oxidative stress, thiol-disulfide homeostasis,

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