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Parasetamol İndüklü Hepatotoksisite Modelinde Kafeik Asit Fenil Ester’ in İnflamasyon ve Oksidatif Stres Üzerine Etkisinin Araştırılması

Parasetamol ucuz olması, kolay bulunabilmesi bakımından sık kullanılan ağrı kesici, ateş düşürücü etkisi olan bir ilaçtır. Parasetamol, karaciğerde metabolize olmaktadır. Yüksek dozda parasetamol karaciğer hasarına (hepatotoksisite) neden olmaktadır. Kafeik Asit Fenil Ester (CAPE); antimikrobik, antiinflamatuvar ve antioksidan özelliklere sahip bir bileşendir. Uzun aromatik ve alifatik yapıdaki karbonları olmasından dolayı hücre duvarından kolayca geçer ve etki edeceği bölgeye daha rahat ulaşır. Bu çalışmanın amacı ratlara parasetamol ile hepatotoksisite oluşturulup meydana gelebilecek oksidatif stres ve enflamasyon üzerine CAPE’nin koruyucu etkisini araştırmak için tasarlanmıştır. Sunulan çalışmada, 36 wistar erkek rat kullanıldı. Rastgele olacak şekilde altı gruba ayrıldı; kontrol grubu; sadece yem verildi, parasetamol grubu; 2 g/kg parasetamol, parasetamol+CAPE grubu; 2 g/kg parasetamol+ 10 μg/kg CAPE, parasetamol+NAC grubu; parasetamol 2 g/kg + NAC (140 mg/kg) ve 140 mg/kg N-Asetilsistein 1 saat sonrasında da 2g/kg dozunda, 2 ml parasetamol, CAPE grubu; 10 mikrogram/kg CAPE ve etanol grubu; CAPE’nin çözdürüldüğü oranda seyreltik etenol. Veriler istatistiksel olarak SPSS-18 ANOVA ile standart sapma olarak değerlendirilmiştir. P<0.05 anlamlı olarak kabul edilmiştir. Çalışmanın sonucunda, parasetamol indüklü hepatotoksisite modelinde kontrol grubuna göre parasetamol grubunda oksidatif stres parametrelerinin ve sitokin seviyelerinin istatistiksel olarak arttığı (P<0.05), CAPE verilen grupla parasetamol grubu karşılaştırıldığında, CAPE’nin meydana gelen enflamasyon ve oksidatif strese karşı koruyucu bir etki gösterdiği gözlemlenmiştir.

Anahtar Kelimeler:

CAPE, Hepatotoksisite, Oksidatif stres, Parasetamol, Rat

Investigation of Caffeic Acid Phenethly Ester Effect on Inflammation and Oxidative Stress in Paracetamol Induced Hepatotoxicity

Paracetamol is a cheap, pain-relieving, fever-reducing medicine that can be easily found. Paracetamol is metabolized in the liver. At high doses, paracetamol causes liver damage hich is called hepatotoxicity. Caffeic Acid Phenyl Ester (CAPE) has antimicrobial, anti-inflammatory and antioxidant properties. Due to its long aromatic and aliphatic carbon structure, it passes easily through the cell wall and reaches the region where it will act more easily. The aim of this study is to understand the CAPE protective effect to hepatoxisitiy oxidative stress and inflamation during the application of paracetamol. In tis study 36 wistar rat was used and they were divided into six groups randomly. Control: given only forage. Paracetamol; 2 g/kg döşe of paracetamol. Paracetamol +CAPE; 2 g/kg döşe of paracetamol + 10 ug/kg CAPE. Paracematol + NAC; Paracetamol 2 g/kg + NAC (140 mg/kg); 140 mg/kg N-asetil Sistein applied after one hour 2 g/kg dose of 2 ml paracetamol. CAPE:10 microgram/kg CAPE and ethanol group. The data is analyzed statistically SpSS-18 ANOVA standard variaton. P<0.05 is accepted meaningful. According to the results obtained, it is seen that hepatoxisity induced paracetamol model has more oxidative stress statistically than the control group (P<0.05). The groups which were given CAPE and paracetamol group when compared, CAPE has protective effect to the occuring inflamation.

Keywords:

CAPE, Hepatotoxicity, Oxidative stres, Paracetamol, Rat,

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