Altın Suriye Hamsterlarında Hiperlipidemik Diyet ile Oluşturulan Karaciğer Hasarı Üzerine Hodan Yağının Etkileri

Bu çalışma, erkek altın Suriye hamsterinda hiperlipidemik diyet ile oluşturulan karaciğer hasarına karşı Gamma Linolenik Asit (GLA) açısından zengin olan hodan yağının hepatoprotektif etkilerini incelenmek için tasarlanmıştır. Bu deneyde, erkek hamsterden oluşan toplam 60 hayvan kullanılmıştır. Bu deney iki aşamada gerçekleştirilmiştir. İlk aşama 4 hafta sürdü, bu aşamada, hamsterler 4 gruba ayrılmıştır. S1 (kontrol), sadece ticari kemirgen diyeti ile beslenmiştir. Diğer gruplar S2, S3 ve S4 ise 80% ticari kemirgen diyeti +%20 yağ içeren diyet ile beslenmiştir (hiperlipidemik diyet). İkinci aşama da yine 4 hafta sürdü, sadece S2 hiperlipidemik diyetle beslenmiştir. S4’e hodan yağı günlük olarak 2g yağ/kg vücut ağırlığı gavaj yoluyla verilmiştir. Hiperlipidemik diyetin alımı, hepatositlerde lipid birikimine neden oldu ve anlamlı olarak (P<0.05) ilk aşama sonunda S2, S3 ve S4 gruplarının her birinde ALT, AST, ALP, LDH, GGT ve CK gibi enzimlerin aktivitesini arttırdı. Bu negatif etkiler sadece S4’te hodan yağı kullanımıyla ortadan kaldırılmıştır.

Anahtar Kelimeler:

Enzim aktiviteleri, Hamster, Hepatositler, Hodan yağı

The Effects of Borage Oil on Hyperlipidemic Diet-induced Liver Damage in Male Golden Syrian Hamsters

This study was designed to examine the hepatoprotective effects of Borage seed oil rich in Gamma Linolenic acid (GLA) against hyperlipidemic diet-induced liver damage in the male golden Syrian hamster. In this experiment a total of 60 animals of male hamsters were used. This experiment was carried out in 2 periods. The first period lasted 4 weeks, in which hamsters were segregated into 4 groups. S1 served as control (given a commercial rodent diet only). Other groups, S2, S3 and S4 were fed with a diet containing of %80 commercial rodent diet + 20% fats (Hyperlipidemic Diet). The second period lasted 4 weeks, in which S2 only that continued fed with hyperlipidemic diet. S4 was orally gavaged with borage oil at 2 g/kg of the body weight daily. Hyperlipidemic diet intake caused lipid accumulation in hepatocytes and significantly (P<0.05) increased the activities of ALT, AST, ALP, LDH, GGT and CK enzymes in each of the S2, S3 and S4 groups at the end of the first period. These negative effects were removed only in S4 by the use of borage oil.

Keywords:

Borage oil, Enzymes activities, Hamster, Hepatocytes,

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