Urtica dioica L. ve Myrtus communis L. Yapraklarının Sulu ve Etanolik Ekstraktlarının Sığır Sindirim Strongilozu Üzerine Antelmintik Aktivitesi: İn-vitro Çalışma

Bu araştırma, doğal olarak enfekte sığırlarda sindirim strongilozu üzerine M. communis ve U. dioica in-vitro antelmintik aktivitesini değerlendirmeyi amaçladı. Ekstraktların antelmintik aktiviteleri, yumurta tarama testi ve larva mortalite testi kullanılarak değerlendirildi. M. communis ve U. dioica'nın yaprak tozu, maserasyon ile özütlendi. Etanolik ve su ekstreleri 0.78, 1.55, 3.1, 6.2, 12.5, 25 ve 50 mg/ml'de test edildi. Albendazol ve DMSO, sırasıyla 20 mg/ml ve %3 konsantrasyonunda pozitif ve negatif kontrol olarak kullanıldı. Regresyon hattı sonuçları, Urtica dioica EE ve EA'nın LC50'sinin sırasıyla 2.57, 3.02 ve 3.22 mg/ml olduğundan oldukça düşük değerlere sahipti; ve embriyonasyon yumurtalarının inhibisyonu, kuluçka hızı ve L1 mortalitesi değerleri sırasıyla, 4.90, 4.67 ve 3.24 mg/ml'idi. Maksimum ortalama embriyonasyon oranı AE ve EE U. dioica'da 25 ve 50 mg/ml konsantrasyonda %100'iken; M. communis, AE ve EE için sırasıyla 50mg/ml konsantrasyonda maksimum % 57.1 ± 3.12 ve % 63.95 ± 3.01 olarak kaydedildi. U. dioica özlerinin yüksek etkisi (%100) her iki ekstrede 25 ve 50 mg/ml ile gözlenirken, M. communis'de ise AE ve EE için 50mg/ml konsantrasyonda yüksek düzeyde kaydedildi (sırasıyla %58.05±3.69 ve 65.13±3.13). U. dioica'nın hem AE hem de EE için larval mortalite oranı, 25 ve 50 mg/ml'deki ekstrelerin 24 saatlik temasında %100 olarak görüldü. Sonuç olarak, bu bulgular U. dioica ve M. communis yapraklarının sulu ve etanolik ekstraktlarının, sığır strongiloz parazit larvaları üzerinde potansiyel bir antelmintik aktiviteye sahip olduğunu göstermiştir.

Anahtar Kelimeler:

Urtica dioica, Myrtus communis, Antelmintik, In-vitro, Sığır, Urtica dioica

Anthelmintic Activity of Aqueous and Ethanol Extracts of Urtica dioica L. and Myrtus communis L. Leaves on Bovine Digestive Strongyles: In-Vitro Study

The present research aimed to evaluate the in-vitro anthelmintic activity of M. communis and U. dioica against digestive strongyles in naturally infected cattle. The anthelmintic activities of the extracts were evaluated using the egg hatch assay and larval mortality assay. Leaves powder of M. communis and U. dioica was extracted by maceration. Ethanolic and water extracts were test at 0.78, 1.55, 3.1, 6.2, 12.5, 25 and 50 mg/ml. Albendazole and DMSO was used as a positive and negative control at concentration 20 mg/ml and 3%, respectively. The results of regression line indicated that LC50 of Urtica dioica EE and EA were very low namely 2.57, 3.02 and 3.22 mg/ml; and 4.90, 4.67 and 3.24 mg/ml for inhibition of embryonation eggs, hatching rate and L1 mortality, respectively. The mean embryonation rate maximum was 100% at 25 and 50 mg/ml concentration in AE and EE U. dioica; whereas M. communis recorded a maximum level of 57.1 ± 3.12% and 63.95 ± 3.01% at 50 mg/ml concentration for the AE and EE, respectively. The extracts of U. dioica leaves high effects (100%) were observed with 25 and 50 mg/ml in both extracts, whereas M. communis recorded a high level at 50mg/ml concentration for the AE and EE (58.05±3.69% and 65.13±3.13%, respectively). The larval mortality rate of both AE and EE from U. dioica showed that the extracts at and 25 and 50 mg/ml exhibited 100 % at 24h of contact. In conclusion, these findings showed that, aqueous and ethanolic extracts of U. dioica and M. communis leaves have a potential anthelmintic activity on eggs and larvae of bovine strongly parasites.

Keywords:

Anthelmintic, Cattle, In-vitro, Myrtus communis, Urtica dioica,

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