Urtica dioica'nın Sıçanlarda Karbon Tetraklorür ile İndüklenen Hepatik Ensefalopati Üzerine Koruyucu Mekanizması

Dünya çapında, Urtica cinsinden türler periferik ve merkezi tıbbi etkileri nedeniyle yaygın olarak kullanılmaktadır; bunlar çay olarak hazırlanır. Son yıllarda, Urtica dioica (UD) türevlerinin yararlı özelliklerini incelemek gittikçe önem kazanmıştır. Bu çalışmanın amacı, UD'nin karbon tetraklorür (CCl4) ile indüklenen hepatik ensefalopatiye (HE) karşı etkilerini değerlendirmektir. Bu çalışmada kırk dokuz yetişkin (2 aylık) erkek Sprague dawley sıçanı kullanıldı. Modeller 8 hafta boyunca intraperitoneal olarak verilen CCI4 (1 mL / kg vücut ağırlığı; haftada iki kez) ile oluşturuldu. Hayvanlar başları kesilerek ötanazi edildi ve sıçan beyinleri histopatolojik değişiklikleri değerlendirmek için çıkarıldı. Biyokimyasal parametreler CCI4 ile tedavi edilen sıçanlardan alınan serum örneklerinde değerlendirildi. UD ekstraktları, kontrol grubuna göre aspartat aminotransferaz (AST), alanin aminotransferaz (ALT), glutamil transferaz (GGT) ve amonyak seviyeleri gibi biyokimyasal serum parametrelerindeki önleyici değişiklikleri arttırarak CCI4'ün neden olduğu beyin hasarına karşı önemli bir koruma sağlamıştır. Hematoksilin-eozin (H & E) boyama ve c-fos ekspresyon metodu kullanılarak beyin dokusunun histopatolojik ve immünohistolojik değişiklikleri gözlendi. Bu çalışmada, UD'nin CCI4 toksisitesi üzerindeki koruyucu etkisi, biyokimya ve immünohistoloji çalışmaları ile gösterilmiştir. UD'nin uygulanması CCI4'ün neden olduğu beyin toksisitesine karşı potansiyel koruyucu etkilere sahip olabilir.

Protective Mechanism of Urtica Dioica on Carbon Tetrachloride-Induced Hepatic Encephalopathy in Rats

Around the world, species from the genus Urtica are commonly used because of their peripheral and central medicinal effects; this is prepared as teas. In recent years, it has become increasingly important to study the beneficial properties of derivatives of Urtica dioica (UD).  The aim of the present study was to evaluate the effects of UD against carbon tetrachloride (CCl4) induced hepatic encephalopathy (HE). Forty-nine adult (2-months-old) male Sprague dawley rats were used in this study. The models were established by CCl4  (1 mL/kg body weight; twice a week) given intraperitoneally for 8 weeks. The animals were euthanized by decapitation and rat brains were removed to assess histopathologic changes. Biochemical parameters were assessed in serum samples from the CCl4-treated rats. UD extracts provided significant protection against CCl4-induced brain damage by increasing the preventing alterations in biochemical serum parameters, such as the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutamyl transferase (GGT) and ammonia relative to the control group. Histopathological and immunohistopathological changes of the brain tissue was observed using Hematoxylin-eosin (H&E) staining and c-Fos expression method. In the present study, the protective effect of UD on CCl4 toxicity was demonstrated through studies of biochemistry and immunohistopathology. Administration of UD may have potential protective effects against CCl4-induced brain toxicity.

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