Zeynep KARAPINAR, Ender DİNÇER, Cumali ÖZKAN

Türkiyede Van Yöresinde Bulunan Köpeklerden Sağlanan Kan ve Rektal Sıvap Örneklerinde Canine Parvovirus-2 Enfeksiyonunun Araştırılması ve Filogenetik Analizi

Bu çalışmada, Van ili Yüzüncü Yıl Üniversitesi Veteriner Fakültesi İç Hastalıkları Kliniğine getirilen Canine Parvovirus (CPV) Enfeksiyonu şüpheli köpeklerden alınan kan ve rektal sıvap örneklerinde PZR tekniği kullanılarak CPV enfeksiyonunun varlığının belirlenmesi ve yerel virusların moleküler karakterizasyonunun yapılması amaçlandı. Klinik olarak enfeksiyon belirtisi gösteren köpeklerden sağlanan 76 adet rektal sıvaptan 57 adedi (%75), 62 adet kan örneğinin ise 40 adedi (%64) PZR ile pozitif olarak belirlendi. Seçilen pozitif örnekler ile virusun moleküler karakterizasyonu gerçekleştirildi. Elde edilen sekans sonucu bilgileri değerlendirildiğinde örneklerin CPV-2a ve CPV-2b grublarında bulunduğu ve kendi aralarında benzerlik gösterdikleri belirlendi. Elde edilen virus suşu sekanslarının Genbankasından sağlanan diğer suşlarla %99-100 oranında homoloji gösterdiği belirlendi.

Anahtar Kelimeler:

CPV, PZR, Moleküler karakterizasyon, Filogenetik analiz

The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal Swab Samples from Dogs in Van Province, Turkey

In this study, it was aimed to determine the presence of CPV infection in the blood and rectal swaps of suspected dogs that they were handed to Yuzuncu Yıl University, Faculty of Veterinary, Department of Internal Disease using PCR techniques and molecular characterization of local viruses. 57 (75%) of the 76 rectal swabs used in the study and 40 (64%) of the 62 blood samples were determined to be positive by PCR obtained from dogs with clinical symptoms. Selected samples were positive with the virus carried the molecular characterization. The results of the sequence analysis were similar among themselves and they were found in CPV-2a and CPV-2b groups. It was determined that the sequences of virus strains showed 99-100% homology with other strains obtained from Genbank.

Keywords:

CPV, PCR, Molecular characterization, Phylogenetic analysis,

PDF

___

Aktaş MS, Özkanlar Y, Kırbaş A (2011). Erzurum ve Çevresinden Kliniğe Getirilen Sahipli Köpeklerin Parvoviral Enteritisini Etkileyen Risk Faktörleri Üzerinde Bir Araştırma. Atatürk Üniversitesi Vet Bil Derg, 6(1), 1-8. Appel MJG, Scott WF, Carmichael LE (1979). Isolation and immunization studies of canine parvo-like virus from dogs with haemorrhagic enteritis. Vet Rec, 105, 156–159. Buonavoglia D, Cavalli A, Pratelli A, Martella V, Greco G, Tempesta M, Buonavoglia C (2000). Antigenic analysis of canine parvovirus strains isolated in Italy. New Microbiol, 23, 93-96. Buonavoglia C, Martella V, Pratelli A, Tempesta M, Cavalli A, Buonavoglia D, Bozzo G, Elia G, Decaro N, Carmichael L (2001). Evidence for evolution of canine parvovirus type 2 in Italy. J Gen Virol, 82, 3021Ð3025. Carmichael LE (1994). Canine parvovirus type-2. An evolving pathogen of dogs. Ann Vet Med, 135, 459-464. Castro TX, Miranda SC, Labarthe NV, Silva LE, Cubel Garcia RCN (2007). Clinical and epidemiological aspects of canine parvovirus (CPV) enteritis in the State of Rio de Janeiro:1995 – 2004. Arq Bras Med Vet Zootec, 59, 333-339. Dei Giudici S, Cubeddu T, Giagu A, Sanna G, Rocca S, Oggiano A (2017). First molecular characterization of canine parvovirus strains in Sardinia, Italy. Arch Virol, 17, 3457-3. Dinçer E (2017). Molecular Characterization and Phylogenetic Analysis of Canine Parvovirus 2 in Dogs, Mersin Province, Turkey. Etlik Vet Mikrobiyol Derg, 28 (2): 96-100. Homem VSF, Mendes YG, Linhares AC (1999). Gastroenterite canina- agentes virais nas fezes de cães diarreicos e não diarreicos. Arq Bras Med Vet Zootec, 51, 531-536. Houston DM, Ribble CS, Head LL (1996). Risk factors associated with parvovirus enteritis in dogs: 283 cases (1982–1991). J Am Vet Med Assoc, 208, 542–546. Kelly WR (1978). An enteric disease of dogs resembling feline panleukopenia. Aust Vet J, 54, 593. Martella V, Decaro N, Buonavoglia C (2006). Genetic and antigenic variation of CPV-2 and implication for antigenic/genetic characterization. Virus Genes, 33:11–3. Nakamura M, Tohya Y, Miyazawa T, Mochizuki M, Phung HT, Nguyen NH, et al. (2004). A novel antigenic variant of canine parvovirus from a Vietnamese dog. Arch Virol, 149: 2261–9. Ntafis V, Xylouri E, Kalli I, Desario C, Mari V, Decaro N, Buonavoglia C (2010). Characterization of Canine parvovirus 2 variants circulating in Greece. J Vet Diagn Invest, 22, 737-740. Özkul A, Keleş İ, Karaoğlu T, Çabalar M, Burgu İ (2002). Detection and RFLP Analysis of Canine Parvovirus (CPV) DNA by Polymerase Chain Reaction (PCR) in a Dog. Turk J Vet Anim Sci, 26: 1201-1203. Parrish CR, O’Connel PH, Evermann JF, Carmicheal LE (1985). Natural variation of canine parvovirus. Science, 230, 1046-1048. Parrish CR, Aquadro CF, Strassheim ML, Evermann JF, Sgro JY, Mohammed HO (1991). Rapid antigenic-type replacement and DNA sequence evolution of canine parvovirus. J Virol, 65: 6544–52. Pérez R, Francia L, Romero V, Maya L, Lopez I, Hernandez M (2007). First detection of canine parvovirus type 2c in South America. Vet Microbiol, [Epub ahead of print]. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013). MEGA6: Molecular Evolutionary Genetics Analysis ver¬sion 6.0. Mol Biol Evol, 30, 2725 - 2729. Timurkan MÖ, Oğuzoğlu TÇ (2015). Molecular characterization of canine parvovirus (CPV) infection in dogs in Turkey. Veterinaria Italiana, 51 (1), 39-44 Torun S, Yılmaz Z, Pratelli A (2005). A Serological Evidence of Minute Virus of Canines (MVC; Canine Parvovirus Type-1) in Dogs in Turkey. Turk J Vet Anim Sci, 29: 923-925. Yılmaz Z, Pratelli A, Torun S (2005). Distribution of Antigen Types of Canine Parvovirus Type 2 in Dogs with Hemorrhagic Enteritis in Turkey. Turk J Vet Anim Sci, 29: 1073-107.