Volkan GELEN, Fikret ÇELEBİ, Emin ŞENGÜL, Ali ÇINAR

Rosmarinus officinalis L. Sulu Ekstraktının İzole Edilmiş Rat Midesinde Mide Asit Salgısı Üzerine Etkisi

Bu çalışmada Rosmarinus officinalis L. (Rosemary) sulu ekstraktının rat mide asit salgısı üzerine in vitro etkisi araştırıldı. Araştırmada 24 adet Sprague Dawley cinsi ergin erkek rat kullanıldı. Ratlar ketamine hydrochloride (Ketalar: 75 mg/kg) ve xylazine (Rompun: 15 mg/kg) anestezisi eşliğinde servikal dislokasyon metodu ile ötenazi edilerek mideleri izole edildi. İzole edilen mideler izole organ banyosunda 1 saat inkubasyon periyoduna bırakıldılar. Bu periyodun sonunda, midelerden elde edilen mukozal sıvının pH’sı bazal pH olarak değerlendirildi. Daha sonra banyo ortamına Histamin uygulanarak mide asit salgısı indüklendi ve mide içeriğinin pH değerleri ölçüldü. Rosemary ekstraktının dört farklı dozu (150, 300, 600 ve 1200 µg/ml) banyoya ayrı ayrı uygulandı ve mide içeriklerinin pH’sı kaydedildi. Ayrıca Rosemary ekstraktının dört farklı dozu tek tek banyoya Histaminle ile birlikte uygulandı ve mide içeriklerinin pH değerleri belirlendi. Elde edilen pH değerleri, bazal pH ile karşılaştırıldı ve ∆pH değerleri hesaplandı. Sonuç olarak, Rosemary ekstraktının 150 µg/ml dozunun mide asit salgısı üzerine etkisinin istatistiksel olarak anlamlı olmadığı ve 300, 600 ve 1200 µg/ml dozlarının mide asit salgısını istatistiksel olarak anlamlı şekilde artırdığı belirlendi.

Anahtar Kelimeler:

Rosemary, Histamin, Rat, Mide Asit Salgısı

The Effect of Rosmarinus officinalis L. Aqueous Extract on Gastric Acid Secretion in Isolated Rats Stomach

In this study, the in vitro effect of Rosmarinus officinalis L. (Rosemary) aqueous extract on rat gastric acid secretion was investigated. 24 male Sprague Dawley male rats were used in the study. Rats were euthanized by cervical dislocation in the presence of ketamine hydrochloride (Ketalar: 75 mg / kg) and xylazine (Rompun: 15 mg / kg) anesthesia. The isolated stomachs were left in the isolated organ bath for 1 hour for the incubation period. At the end of this period, the pH of the mucosal fluid obtained from the stomachs was evaluated as the basal pH. Histamine was then applied to the bath environment to induce stomach acid secretion and the pH values of the stomach contents were measured. Four different doses of Rosemary extract (150, 300, 600 and 1200 μg / ml) were applied to the bath separately and the pH of the stomach contents was recorded. In addition, Rosemary Extract's four separate doses were applied to the individual baths with Histamine and the pH values of the stomach contents were determined. The resulting pH values were compared to the basal pH and the ΔPH values were calculated. In conclusion, the effect of 150 μg / ml dose of Rosemary extract on gastric acid secretion was not statistically significant and doses of 300, 600 and 1200 μg / ml increased stomach acid secretion statistically significantly.

Keywords:

Rosemary, Histamine, Rat, Gastric acid secretion,

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