Zafer CANTEKİN, Gamze Özge ÖZMEN, Melek DEMİR, Zeynep YILMAZ ER, Hasan SOLMAZ, Yaşar ERGÜN

Hatay Bölgesinde Keçi Mastitis Etkenlerinin ve Etkenlerin Antibiyotik Dirençlerinin Belirlenmesi

Birçok ülkede keçi yetiştiriciliği belirli bir ekonomik öneme sahiptir. Son yıllarda keçi ürünlerine yönelik bir eğilim vardır. Süt kalitesi meme içi infeksiyonlar tarafından etkilenen somatik hücre sayısı ile ilişkilidir. Bu çalışmanın amacı, keçilerde mastitise neden olan bakteri, maya ve mantarların izolasyonu ve identifikasyonu ve ayrıca izole edilen bakterilerin antibiyotik duyarlılıklarının belirlenmesidir. Bu amaçla, 11 farklı keçi çiftliğinde 110 keçiden alınan toplam 220 süt örneğinde etken izolasyonu ve identifikasyonu yapıldı. Ayrıca izole edilen bakteriyel izolatların antibiyotik dirençleri disk diffüzyon testi ile araştırıldı. Çalışmada toplanan 220 adet süt örneğinin 28 (%12.73)’inden 30 adet bakteri izole edildi. Bu 30 izolatın, 15 (%50)’i koagülaz negatif stafilokok, 8 (%26.67)’i S. aureus, 5 (%16.67)’i, S. uberisve 2 (%6.67)’si de E. coliolarak identifiye edildi. İdentifikasyonlar, izolatların direkt kültürlerinden yapılan moleküler analizler ile teyit edildi. Bu suşlarda en yüksek antibiyotik direnci penisilin 21 (%70) ve amoksisilin 19 (%63.33)’e karşı bulundu, buna karşın izolatların 30 (%100)’u gentamisin’e, 28 (%93.3)’i enrofloksasin’e, 28 (%93.3)’i oksitetrasiklin’e ve 27 (%90)’si de amoksisilin + klavulanik asit’e duyarlı bulundu. Yapılacak periyodik çalışmalarla keçi mastitislerinden etkenlerin ve antibiyotik direnç durumlarının belirlenmesi hastalığın tedavi ve korunmasında oldukça yararlı veriler sağlayabilecektir.

Anahtar Kelimeler:

Antibiyotik direnci, Keçi sütü, Mastitis

Detection of Causative Agents in Goat Mastitis and their Antibiotic Resistance in Hatay Region

Goat farming in many countries has significant economic importance. In recent years,thereisa trendtogoat products. Milk quality is associated with somatic cell counts that can be affected intra-mammary infections. The aim of this study is isolation and identification of causative bacterial, yeast and fungal mastitis agents in goat mastitis and detection of antibiotic resistance in bacterial isolates. Totally 220 milk samples from 110 goats in 11 farms were studied for isolation and identification of the agents. And antibacterial resistance of isolated microorganism were studied by disk diffusion method. Totally 30 strains isolated from samples and identification, isolation was made from 28 (12.73%) of 220 milk samples. Among these 30 isolates, 15 (50%) Coagulase Negative Staphylococci, 8 (26.67%) S. aureus, 5 (16.67%) S. uberisand 2 (6.67%) E. coliwas identified. Identification was confirmed by molecular analyses from the direct culture of isolates. The highest resistance were found against to penicillin 21 (70%) and amoxicillin 19 (63.33%), and isolates highly sensitive to gentamicin 30 (100%), enrofloxacin 28 (93.3%), oxytetracycline 28 (93.3%) and amoxicillin + clavulanic acid 27 (90%). Periodical studies and constant monitoring of antibiotic resistance might be beneficial for therapy and prevention strategies and useful management acquisition of antibiotic resistance.

Keywords:

Antibiotic resistance, Goat milk, Mastitis,

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