Deneysel Diyabet Oluşturulan Ratlarda Prooksidan / Total Antioksidan Durumu ve Vitamin Düzeyleri Üzerine Likopenin Etkisi

Bu çalışma, deneysel olarak diyabet oluşturulan ratlarda antioksidan olarak koruyuculuğu bilinen likopen uygulanmasının TBARS, TAS ve vitamin (α-tokoferol, retinol, D3) seviyeleri üzerine etkilerini ortaya koymak amacıyla planlandı. Diyabet oluşturmak için D ve D+L grubundaki ratlara 45 mg/kg tek doz streptozotosin (STZ) intraperitoneal (i.p.) uygulandı. Kan şekerleri 270 mg/dL ve üzerinde olanlar diyabetik olarak kabul edilip çalışmaya dahil edildi. L ve D+L grubundaki ratlara likopen ayçiçeği yağında çözdürülerek 10 mg/kg/gün olarak uygulandı. Dört haftalık deneme süresinden sonra, kontrol ve likopen gruplarına göre diyabet grubunda TBARS, HbA1c ve glukoz seviyesinin arttığı, buna karşın TAS, retinol ve α-tokoferol düzeylerinin azaldığı tespit edildi (P<0.001). Diyabet grubuna göre diyabet oluşturulup likopen verilen grupta istatistiksel açıdan önem arz etmeyecek şekilde (P>0.05) TBARS seviyesinin düştüğü, TAS ve α-tokoferol seviyesinin yükseldiği, HbA1c ve glukoz seviyesinin ise istatistiksel olarak önem gösterecek şekilde düştüğü (P<0.001) yine de kontrol grubundan yüksek olduğu, retinol seviyesinin kontrol grubu ile aynı olduğu bulundu. Vitamin D3 seviyeleri açısından gruplar arasında fark olmadığı saptandı (P>0.05). Elde edilen sonuçlar diyabetik kişilerin diyetlerinde likopen ihtiva eden gıdaları tüketmelerinin, diyabetin neden olduğu hücre hasarının önlenmesinde görev alan antioksidan sistemleri güçlendirebileceği kanısına varıldı.

The Effects of Lycopene on Prooksidant/Total Antioksidant Status and Vitamin Levels in Experimental Diabetic Rats

The aim of this study, lycopene known as antioxidant was applied to rats with experimental diabetes to determine the effects of TBARS, TAS and vitamins (α-tocopherol, retinol, D3) levels. In order to induce diabetes in the rats D and D+L groups were given 45 mg/kg single dose streptozotocin (STZ) intraperitoneally (i.p.); lycopene dissolved in sunflower oil was administered (10 mg/kg/day) to the rats in the L and D+L groups. Blood glucose rise 270 mg/dl those with diabetes can be considered to be enrolled in the study. After the four-week experiment, there was increase in the TBARS, HbA1c and glucose levels while in decrease TAS, retinol an α-tocopherol levels in diabetic group were significanly different compared to the control and lycopene groups (P<0.001). Accordig to diabetic group, in the lycopene+diabetic group the decrease in TBARS level and an increase in TAS and α-tocopherol levels were seen, these alternations were not sufficient to constitute a statistical importance,  retinol levels in the lycopene+diabetic group were similar to control group. According to diabetic group, HbA1c glucose levels were decreased in the lycopene+diabetic group (P<0.001), but these levels were higher than control group. The results suggest that consuming food include lycopene in diets of diabetic humans may serve to reinforce the antioxidant systems, thus having a protective effect against cell damage by diabetes.

___

  • Agarwal S, Rao AV (1998). Tomato lycopene and low density lipoprotein oxidation, a human dietary intervention study. Lipids, 33, 981-984. Akkuş İ (1995). Serbest Radikaller ve Fizyopatolojik Etkileri, Mimoza Yayınları. Konya, 38. Anderson RA, Roussel AM, Zouari N, Mahjoub S, Matheu JM, Kerkeni A (2001). Potential antioxidant effect of zinc and chromium supplementation in people with type II diabetes mellitus. Am J clin nutr, 20(3), 212-218. Aydın M (2008). Ratlarda diyabetik nöropatide rol oynayan oksidatif hasarın önlenmesinde likopenin etkinliği. MKÜ, Biyokimya AD, Yüksek Lisans Tezi. Basu TK, Basualdo C (1999). Vitamin A homeostasis and diabetes mellitus. J Nutr, 15, 156. Beverly JZ, Sarı C (1983). Detemination of alpha-tocopherol in tissues and plasma by high-performance liquid chromatography. Anal Biochem, 130, 146-150. Bramley P M (2000). Lycopene benefical to human health. Phyt, 54, 233-256. Breinholt V, Lauridsen ST, Daneshvar B, Jakobsen J (2000). Dose-response effects of lycopene on selected drug-metabolizing and antioxidant enzymes in the rat. J Canc Lett, 154(2), 201-210. Briviba K, Schna K, Rechkemmer G, Bub A (2004). Supplementation of a diet low in carotenoids with tomato or carrot juice does not affect lipid peroxidation in plasma and feces of healthy men. J Nutr, 134, 1081-1083. Bub A, Watzl B, Abrahamse L ve ark. (2000). Moderate intervention with carotenoid-rich vegetable products reduces lipid peroxidation in men. J Nutr, 130, 2200-2206. Cengiz M, Cengiz S (2000). Tip 2 diyabetli hastalarda C vitamini uygulamasının eritrosit glutatyon ve HbA1c düzeyleri üzerine etkisi. Cerrahpaşa Tıp Derg, 31, 211-215. Ceriolla A, Bortolotti N, Pirisi M (1997). Total plasma antioxidant capacity predicts thrombosis-prone status in NIDDM patients. Diabetes Care, 20, 1589-1593. Chandra Mohan KVP, Nagini S (2003). Dose-response effects of tomato lycopene on lipid peroxidation and enzymic antioxidants in the hamster buccal pouch carcinogenesis model. J Nutr res, 23(10), 1403-1416. Çelik S, Yılmaz Ö (2003). Diyabetik ratlarda vitamin E’nin serum lipitleri ve lipit peroksidasyonu üzerine etkisi. Türk J Biology, 23, 39-46. Dixon ZR, Shie FS, Warden BA, Burri BJ, Neidlinger TR (1998). The effect of a low carotenoid diet on malondialdehyde-thiobarbituric acid (MDA-TBA) concentrations in women: a placebo-controlled double-blind study. Am J Coll Nutr, 17, 54-58. Duzguner V, Kaya S (2007). Effect of zinc on the lipid peroxidation and the antioxidant defense systems of the alloxan-induced diabetic rabbits. Free Radic Biology Med, 42(10),1481-1486. Facchini FS, Humphreys MH, DoNascimento CA, Abbasi F, Reaven GM (2000). Relation between insulin resistance and plasma concentrations of lipid hydroperoxides, carotenoids, and tocopherols. Am J Clin Nutr, 72, 776–779. Faure P, Corticelli P, Richard MJ ve ark. (1993). Lipid peroxidation and trace element status in diabetic ketotic patients: influence of insulin therapy. Clin Chem, 39, 789-793. Fidan AF (2007). Deneysel diyabet oluşturulmuş ratlarda diyete katılan farklı yapılardaki saponin içerikli bitkilerin DNA hasarı, protein oksidasyonu ve lipid peroksidasyonu ile bazı biyokimyasal parametrelere etkilerinin araştırılması. AKÜ. Biyokimya AD, Doktora Tezi. Gallou G, Ruelland A, Legras B, Maugendre D (1993). Pasma malondialdehyde in type 1 and type 2 diabetic patients. Clin Chim Acta, 214(2), 227-234. Gumieniczek A, Hopkala H, Wojtowicz Z, Nikolajuk J (2002). Changes in antioxidant status of heart muscle tissue in experimental diabetes in rabbits. Acta Biochimica Polonica, 49(2), 529-535. Halifeoğlu İ, Karataş F, Çolak R, Canatan H, Telo S (2005). Tip II diyabetik hastalarda tedavi öncesi ve tedavi sonrası oksidan ve antioksidan durum. Fırat Tıp Derg, 10(3), 117-122. Jain SK, Levine SN, Duett J, Hallier B (1990). Elevated lipid peroxidation levels in red blood cells of streptozotocin treated diabetic rats. Metabolism, 39, 971-975. Jain SK, McVie R, Duett J, Herbst JJ (1999). Erythrocyte membrane lipid peroxidation and glicosylated hemoglobin in diabetes. Diabetes, 38(12), 1539-1543. Kenneth WM, Chung SY (1985). An isocratic high-performance liquid chromatography method for the simultaneous analysis of plasma retinol, α-tocopherol and various carotenoids. Anal Biochem, 145, 21-26. Knekt P, Reunanen A, Marniemi J, Leino A, Aromaa A (1999). Low vitamin E status is a potential risk factor for insulin-dependent diabetes mellitus. J Intern Med, 245(1), 99-102. Kuyvenhoven JP, Meınders AE (1999). Oxidative stress and diabetes mellitus, Pathogenesis of long term complications. Eur Intern Med, 10, 9-19. Maritim A, Dene BA, Sanders RA (2003). Effects of pycnogenol treatment on oxidative stress in streptozotocin induced diabetic rats. J Biochem Mol Toxicol, 17(3), 193-199. Maxwell S R (1995). Prospects for the use of antioxidant therapies. Drug, 49, 345-361. Maxwell SR, Thomason H, Sandler D (1997). Antioksidant status in patients with uncomplicated insulin dependent and-non insulin diabetes mellitus. Eur Clin Inv, 27, 484-490. Mellert W, Deckardt K, Gembardt C, Schulte S, Van Ravenzwaay B, Slesinski RS (2002). Thirteen-week oral toxicity study of synthettic lycopene products in rats. Food Chem Toxicol, 40, 1581-1588. Merzouk S, Hichami A, Madani S, Merzouk H, Berrouiguet AY (2003). Antioxidant status and levels of different vitamins determined by high performance liquid chromatography in diabetic subjects with multiple complications. Gen Physiol Biophys, 22(1), 15-27. Pozzilli P, Manfrini S, Crino A, Picardi A, Leomanni C (2005). Low levels of 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3 in patients with newly diagnosed type I diabetes. Horm Metab Res, 37(11), 680-683. Quinn L (2002). Mechanism in the development of type II diabetes mellitus. J Cardiovasc Nurs, 16(2), 1-16. Rafi MM, Yadav PN, Reyes M (2007). Lycopene inhibits LPS-induced proinflammatory mediator inducible nitric oxide synthase in mause macrophage cells. J Food Sci, 72 (1), 69- 74. Rajurkar NS, Pardeshi M (1997). Analysis of some herbal plants from India used in the control of diabetes mellitus by NAA and AAS techniques. Appl Radiat Isot, 48(4), 1059-1062. Salonen JT, Nyyssonen K, Tuomainen TP (1995). Increased risk of non insulin dependent diabetes mellitus at low plasma vitamin E consentrations, a four year follow up study in men. Br Mes, 311, 1124-1127. Scragg R, Holdaway I , Singh V, Metcalf P, Baker J (1995). Serum 25-hydroxyvitamin D3 levels decreased in impaired glucose tolerance and diabetes mellitus. DRCP, 27(3), 181-188. Seghrouchni I, Drai J, Bannier E ve ark. (2002). Oxidative stress parameters in type I, type II and insulin-treated type 2 diabetes mellitus, insulin treatment efficiency. Clin Chim Acta, 321, 89-96. Sheweita SA, Newairy AA, Mansour HA, Yousef MI (2002). Effect of some hypoglycemic herbs on the activity of phase I and II drug-metabolizing enzymes in alloxaninduced diabetic rats. Toxicol, 174(2), 131-139. Sindhu RK, Koo JR, Roberts CK, Vaziri ND (2004). Dysregulation of hepatic SOD, CAT and GPx in diabetes, response to insulin and antioxidant therapy. Clin Exp Hypertens, 26(1), 43-53. Steinberg FM, Chait A (1998). Antioxidant vitamin supplementation and lipid peroxidation in smoker. Am J Clin Nutr, 68, 319-327. Stewart LR, Judd HJ (1984). Rapid procedure for the determination of vitamins A and D in fortified skimmed milk powder using high-performance liquid chromatography. T Analyst, 109, 489-492. Sundaram RK, Bhaskar A, Vijayalingam S, Viswanathan M, Mohan R, Shanmugasundaram KR (1996). Antioxidant status and lipid peroxidation in type II diabetes mellitus with and without complications. Clin Sci, 90, 255-260. Şekeroğlu MR, Sahin H, Dülger H, Algün E (2000). The effect of dietary treatment on erythrocyte lipid peroxidation, superoxide dismutase, glutathione peroxidase, and serum lipid peroxidation in patients with type 2 diabetes mellitus. Clin Biochem, 33, 669-674. Tajiri Y, Grill VE (1999). Interactions between vitamin E and glucose on β-cell functions in the rat, an in vivo and invitro study. Pancreas, 18, 274-281. Tas S, Sarandol E, Ziyanok-Ayvalik S (2006). Vanadyl sulfate treatment improves oxidative stress and increases serum paraoxonase activity in streptozotocin-induced diabetic rats. Nutr Res, 26, 670–676. Uchiyama S, Yamaguchi M (2005). Oral administration of β -cryptoxanthin prevents bone loss in streptozotocin-diabetic rats in vivo. Biol Pharmacol Bull, 28, 1766- 1769. Uzel N, Sivas A, Uysal M (1987). Erythrocyte lipid peroxidation and glutathione peroxidase activities in patients with diabetes mellitus. Horm Metabol Res, 19, 89-90. Valabhji J, McColl AJ, Richmond W (2001). Total antioxidant status and coronary artery calcification in type 1 diabetes. Diabetes Care, 24, 1608-1613. Vantyghem MC, Balduyck M, Zerimech F ve ark. (2000). Oxidative markers in diabetic ketoacidosis. Endocrinol Invest, 23, 732-736. Vardı N, Iraz M, Öztürk F ve ark. (2005). Deneysel diyabetin sıçan böbreklerinde meydana getirdiği histolojik değişiklikler üzerine melatoninin iyileştirici etkileri. İÜ Tıp Fak Derg, 12, 45-152. Wang HX, Ng TB (1999). Natural products with hypoglycemic, hypotensive, hypocholesterolemic, antiatherosclerotic and antithrombotic activities. Life Sci, 65(25), 2663-2677. West I (2000). Radicals and oxidative stres in diabetes. Diabet Med, 17, 171-180. Yanbeyi S (1999). Aspirin ve antioksidant buthylated hydroxyanisole’ün tavşanlarda eritrosit total katalaz, süperoksit dismutaz ve glutatyon peroksidaz aktiviteleri üzerine etkileri. OMÜ, Biyoloji AD, Doktora Tezi. Yu BP (1994). Cellular defenses against damage from reactive oxygen species. Physiol Rev,74, 139-162.