Van ve Yöresinde Sağlıklı ve Kısır Morkaraman Irkı Koyunlarda Kan Serumunda Cinsiyet Hormonlarının FSH, Östradiol-17 B, Progesteron RİA ile Tesbiti ve Kısırlık ile İlişkilerinin Araştırılması

Bu çalışmada, Morkaraman ırkı35 adet gebe, 15 adet kısır koyun iki grup hallnde kullanıldı. Bu koyunlarda döl verimi ve gebeliğin devamlılığını sağlayan FSH, östradiol-17 /3 E2 ve progesteron Pf hormonlarının, gebelik süresince, RIA yöntemiyle ile kan serumu seviyelerine bakıldı. FSH hormon miktarı gebe koyunlarda koç katımı zamanında 1064 ±10 mlU/ml, 43.günde 1041 ± 6 mlU/ml, 86.günde 1013 ± 8 mlU/ml, 129.günde 1025 ± 16 mlU/ml doğum sonrasında 1001 ± 6 mlU/ml, kısır koyunlarda sırasıyla 1008 ± 12 mlU/ml, 986 ± 10 mlU/ml, 1007 ± 6 mlU/ml, 1005 ± 10 mlU/ml, 1010 ±8 mlU/ml olarak belirlendi. Östradiol-17 3 E2 kısır koyunlarda koç katımı zamanında 28.25 +4.7 pg/ml, 43. günde 29.0 ±3.5 pg/ml, 86. günde 22.1 ±2.8 pg/ml, 129. günde 30.0+2.0pg/ml, doğum sonrasında 27.0+3.1 pg/ml; gebe koyunlarda sırasıyla 49.66 ±8.3 pg/ml, 31.11 ±1.3pg/ml, 44.6 ±3.3pg/ml, 82.0 ±8.0pg/ml, 29.0 ±2.9pg/ml olarak ölçüldü. Progesteron Pf gebe koyunlarda koç katım zamanında 1.95 ±0.01 ng/ml, 43.günde 3.90 ±2.1 ng/ml, 86. günde 5.36 ± 2.8 ng/ml, 129. günde 7.18 ±6.4 ng/ml, doğum sonrasında 0.15 ± 0.01 ng/ml; kısır koyunlarda sırasıyla 0.42 ±0.02 ng/ml, 0.39 ± 0.02 ng/ml, 0.40 ± 0.01 ng/ml, 0.38 ± 0.01 ng/ml, 0.38 ± 0.01 ng/ml olarak saptandı. Serum FSH değerleri gruplar arasında sırasıyla l. ve 2. kan ahmlan zamanında, gebe olan grubun kendi içerisinde ise l. ve 2. ile 3. ve 5. kan alım zamanlarındaki değerler arasındaki fark istatistiki açıdan anlamlı bulundu P

Anahtar Kelimeler:

Koyun, FSH, Ötradiol 17-/3, Progesteron, Kısırlık

Determination of Sexual Hormones FSH, Oestradiol-17 /3, Progesterone by RIA in the Plasma of Healthy and Sterile Morkaraman Sheep in andAround Van and Their Relationship with Sterility.

Determination of Sexual Hormones FSH, Oestradiol-17 /3, Progesterone by RIA in the Plasma of Healthy and Sterile Morkaraman Sheep in andAround Van and Their Relationship with Sterility. In this study, thirty-five pregnant and fıfteen sterile Morkaraman ewes vvere used as two groups . Blood serum of FSH, oestrogen and progesterone hormones required for the continuation of pregnancy and fertility were detected during the pregnancy of the evves by RIA. FSH hormone levels of pregnant ewes vvere detected as 1064 ±10 mlU/ml on the mating season, 1041 ±6 mlU/ml, on the 43th day 1013 ±8 mlU/ml, on the 86 th day 1025 ±16 mlU/ml, on the 129th day, 1001 ±6 mlU/ml on the after birth; FSH levels of sterile ewes yvere as follovvs 1008 ±12 mlU/mL, 986 ± 10 mlU/ml, 1007 ± 6 mlü/ml, 1005 ±10 mlU/ml, and 1010 ± 8 mlU/ml consequently. Oestradiol-17 3 E2 levels of sterile evves yvere detected as 28.25 ±4.7 pg/ml on the mating season, 29.0 ±3.5 pg/ml on the 43th day, 22.1 ±2.8 pg/ml on the 86 th day, 30.0 ±2.0 pg/ml on the 129 th day, 27.0 ±3.1 pg/ml on the after birth; Oestradiol 17 /3 E^ levels of pregnant eyves yvere detected as folloyvs 49.66 i 8.3 pg/ml, 31.11 i 1.3pg/ml, 44.6 i 3.3 pg/ml, 82.0 i 8.0 pg/ml and 29.0 + 2.9 pg/ml consequently. Progesterone Pf levels of pregnant eyves yvere found as 1.95 ± 0.01 ng/ml, 3.90 d: 2.1 ng/ml, 5.36 d: 2.8 ng/ml, 7.18 d: 6.4 ng/ml, 0.15 d: 0.01 ng/ml, progesterone levels of sterile eyves yvere detected as 0.42 d: 0.02 ng/ml, 0.39 d: 0.02 ng/ml, 0.40 i 0.01 ng/ml, 0.38 d: 0.01 ng/ml and 0.38 d: 0.01 ng/ml consequently. Serum FSH levels betyveen tyvo groups yvere signifıcant during the first and second blood taken times consequently; hoyvever, serum FSH levels yvithin the group of pregnant eyves betyveen first and second, and third andfifth blood taken times yvere found statistically significant P

Keywords:

Sheep, FSH, Oestradiol-17 /3, Progesterone, Sterility,

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