Fasulye Tohumunun Biyofortifikasyonu için Mineral İçerik Vasyasyonlarının Değerlendirilmesi
Genetik kaynaklar ıslahçıların yüksek verim ve mineral içeriğine sahip yeni çeşitler geliştirebilmesi için çok önemlidir. 2015-2016 yılları arasında Türkiye’nin Batı Anadolu bölgesinin farklı bölgelerinden seksen üç Phaseolus populasyonu toplanmıştır. Morfolojik karakterizasyon sonuçları ve tartılı derecelendirmeye göre yirmi fasulye hattı 2016 yılında seçilmiştir. Yirmi fasulye hattının ve iki ticari çeşidin fosfor, potasyum, bakır, çinko, mangan, demir, kalsiyum ve magnezyum içerikleri tarla koşullarında test edilmiştir. Deneme 2017 yılı yetiştirme periyodunda Bolu Abant İzzet Baysal Üniversitesi, Araştırma ve Uygulama Alanı’nda tesadüf blokları deneme desenine göre üç tekrarlamalı olarak yürütülmüştür. Sonuçlar, fasulye hat ve çeşitleri arasında P (%0.94−1.30), K (%2.38-3.59), Cu (7.80-14.80 mg kg-1), Zn (19.74-66.68 mg kg-1), Mn (7.46-27.25 mg kg-1), Fe (48.98-182.45 mg kg-1), Ca (0.18-0.48 mg kg-1) ve Mg (0.56-0.71 mg kg-1) yüksek oranda varyasyon göstermiştir. K ve Zn (r=0.447; P
Assessment of Mineral Content Variations for Biofortification of the Bean Seed
Germplasm collections are very important for breeder to develop new cultivars with highmineral nutrients and yield. Eighty-three Phaseolus landraces were collected from different provincesof Western Anatolia Region of Turkey in 2015-2016. Twenty common bean lines were selectedaccording to morphological characterization results and weighted scaling method in 2016. Phosphorus(P), potassium (K), copper (Cu), zinc (Zn), manganese (Mn), iron (Fe), calcium (Ca), and magnesium (Mg)contents of these twenty common bean lines and two commercial cultivars were tested under fieldconditions. Randomized block design with three replicates was used for analysis in 2017 growingseason on the experimental farm of Bolu Abant Izzet Baysal University. The results showed high levelof variation among lines and cultivars in terms of P (0.94-1.30%), K (2.38-3.59%), Cu (7.80-14.80 mgkg-1), Zn (19.74-66.68 mg kg-1), Mn (7.46-27.25 mg kg-1), Fe (48.98-182.45 mg kg-1), Ca (0.18-0.48 mgkg-1) and Mg (0.56-0.71 mg kg-1) contents. Positive correlations were found between K and Zn (r=0.447;P
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- Addinsoft 2016. XLSTAT 2016: Data Analysis and Statistics
Software for Microsoft Excel. New York, USA.
- Baloch FS., Karaköy T., Demirbaş A., Toklu F., Özkan H and
Hatipoğlu R., 2014. Variation of some seed mineral
contents in open pollinated faba bean (Vicia faba L.)
landraces from Turkey. Turkish Journal of Agriculture and
Forestry, 38: 591-602.
- Beebe S., Gonzalez A and Rengifo J., 2000. Research on trace
minerals in the common bean. Food and Nutrition
Bulletin, 21: 387-391.
- Bitocchi E., Rau D., Bellucci E., Rodriguez M., Murgia ML.,
Gioia T., Santo D., Nanni L., Attene G and Papa R., 2017.
Beans (Phaseolus ssp.) as a model for understanding crop
evolution. Frontiers in Plant Science, 8: 722.
- Blair MW., Monserrate F., Beebe SE., Restrepo J and Ortubé
J., 2010. Registration of high mineral common bean
germplasm lines NUA35 and NUA56 from the red
mottled seed class. Journal of Plant Growth Regulation,
4: 1-5.
- Blair MW., 2013. Mineral biofortification strategies for food
staples: the example of common bean. Journal of
Agricultural and Food Chemistry, 61: 8287-8294.
- Bremner JM., 1965. Method of Soil Analysis. Part 2. Chemical
and Microbiological Methods, American Society of
Agronomy Inc. USA.
- Broughton WJ., Hernandez G., Blair M., Beebe S., Gepts P and
Vanderleyden J., 2003. Bean (Phaseolus spp.)-model food
legumes. Plant Soil, 252: 55-128.
- Ceyhan E., Harmankaya M and Avcı MA., 2008. Effects of
sowing dates and cultivars on protein and mineral
contents of bean (Phaseolus vulgaris L.). Asian Journal of
Chemistry, 20(7): 5601-5613.
- Çiftçi V., Şensoy S and Türkmen Ö., 2009. Van-Gevaş’ta
Yaygın Olarak Yetiştirilen Yalancı Dermason Fasulye
Populasyonunun Seleksiyon Yöntemiyle Islahı, Project
report (TOVAG- 106O346).
- Çiftçi V., Şensoy S and Kulaz H., 2012. Doğu Anadolu’nun
Güneyinde Yetiştirilen Fasulye Gen Kaynaklarının
Toplanması Ve Değerlendirilmesi, Tübitak Project Report
(109O163).
- Duc G., Bao S., Baum M., Redden B., Sadiki M., Suso MJ.,
Vishniakova M and Zong X., 2010. Diversity maintenance
and use of Vicia faba L. genetic resources. Field Crops
Research, 115: 270-278.
- Dutta SK., Chatterjee D., Sarkar D., Singh SB., Boopathi T.,
Kuotsu R., Vikramjeet K., Akoijam RS., Saha S.,
Vanlalhmangaiha., Malsawmzuali., Chowdhury S and
Lungmuana., 2016. Common bean (Phaseolus vulgaris L.,
Fabaceae), landraces of Lushai hills in India: nutrients and
antioxidants source for the farmers. Indian Journal of
Traditional Knowledge, 15(2): 313-320.
- Elkoca E and Çınar T., 2015. The adaptation, agronomical and
quality characteristcs of some dry bean (Phaseolus
vulgaris L.) cultivars and lines under Erzurum ecological
conditions. Anadolu Tarım Bilimleri Dergisi, 30(2): 141-
153.
- Garg M., Sharma N., Sharma S., Kapoor P., Kumar A.,
Chunduri V and Arora P., 2018. Biofortified crops
generated by breeding agronomy and transgenic
approaches are improving lives of millions of people
around the world. Frontiers in Nutrition, 5: 12.
- Graham RD., Welch RM and Bouis HE., 2001. Addressing
micronutrient malnutrition through enhancing the
nutritional quality of staple foods: principles,
perspectives and knowledge gaps. Advances in
Agronomy, 70: 77-142.
- Gelin JR., Forster S., Grafton SK., McClean PE and Rojas-
Cifuentes GA., 2007. Analysis of seed zinc and other
minerals in a recombinant inbred population of navy
bean (Phaseolus vulgaris L.). Crop Science, 47: 1361-13.
- Gepts PTC., Osborn K., Rashka K and Bliss FA., 1986.
Phaseolin protein variability in wild forms and landraces
of the common bean (Phaseolus vulgaris): evidence for
multiple centers of domestication. Economic Botany, 40:
451-468.
- Gesto-Seco EM., Moreda-Pineiro A., Bermejo-Barrera A and
Barrera-Bermejo P., 2009. Multi-element determination
in raft mussels by fast microwave-assisted acid leaching
and inductively coupled plasma-optical emission
spectrometry. Talanta, 72: 1178-1185.
- Islam FMA., Basford KE., Jara C., Redden RJ and Beebe SE.,
2002. Seed compositional and disease resistance
differences among gene pools in cultivated common
bean. Genetic Resources and Crop Evolution, 49: 285-
293.
- Kacar B and İnal A., 2008. Bitki Analizleri. Nobel Yayın
Dağıtım, Ankara.
- Kantar F., Elkoca E., Eken C and Dönmez F., 2010. Kuzey Doğu
Anadolu Bölgesi ve Çoruh Vadisi’nde Yetiştirilen Kuru
Fasulye Gen Kaynaklarının Toplanması ve
Değerlendirilmesi. Tubitak Project Report (107O400).
- Karaköy T., Erdem H., Baloch FS., Toklu F., Eker S., Kilian B and
Özkan H., 2012. Diversity of macro-and micronutrients in the seeds of lentil landraces. The Scientific World
Journal, 2012: 1-9.
- Karaköy T and Demirtaş A., 2017. Sivas ekolojik koşullarında
yetiştirilen Türkiye orijinli yerel bezelye (Pisum sativum L.)
genotiplerinin bazı besin elementi içerikleri bakımından
değerlendirilmesi. Adnan Menderes Üniversitesi Ziraat
Fakültesi Dergisi, 14(2): 7-11.
- Khan MA., Fuller MP and Baloch FS., 2008. Effect of soil
applied zinc sulphate on wheat (Triticum aestivum L.)
grown on a calcareous soil in Pakistan. Cereal Research
Communications, 36: 571-582.
- Kwak M and Gepts P., 2009. Structure of genetic diversity in
the two major gene pools of common bean (Phaseolus
vulgaris L., Fabaceae). Theoretical and Applied Genetics,
118: 979-992.
- Madakbaş SY and Ergin M., 2011. Morphological and
phenological characterization of Turkish bean (Phaseolus
vulgaris L.) genotypes and their present variation states.
African Journal of Agricultural Research, 6(28): 6155-
6166.
- Moraghan JT and Grafton K., 2001. Genetic diversity and
mineral composition of common bean seed. Journal of
the Science of Food and Agriculture, 81: 404-408.
- Murphy J and Riley JP., 1962. A modified single solution for
the determination of phosphate in natural waters.
Analytica Chimica Acta, 27: 31-36.
- Nadeem MA., Habyarimana E., Çiftçi V., Nawaz MA., Karaköy
T., Comertpay G., Shahid MQ., Hatipoğlu R., Yeken MZ.,
Ali F., Ercişli S., Chung G and Baloch FS., 2018.
- Characterization of genetic diversity in Turkish common
bean gene pool using phenotypic and whole-genome
DArTseq-generated silicoDArT marker information. PloS
one, 13(10): e0205363.
- Petry N., Boy E., Wirth JP and Hurrell RF., 2015. Review: the
potential of the common bean (Phaseolus vulgaris) as a
vehicle for iron biofortification. Nutrients, 7(2): 1144-
1173.
- Pinheiro C., Baeta JP., Pereira AM., Domingues H and Ricardo
CP., 2010. Diversity of seed mineral composition of
Phaseolus vulgaris L. germplasm. Journal of Food
Composition and Analysis, 23(4): 319-325.
- Schmutz J., McClean PE., Mamidi S., Wu GA., Cannon SB.,
Grimwood J., Jenkins J., Shu S., Song Q., Chavarro C.,
Torres-Torres M., Geffroy V., Moghaddam S., Gao D.,
Abernathy B., Barry K., Blair M., Brick MA., Chovatia M.,
Gepts P., Goodstein DM., Gonzales M., Hellsten U., Hyten
DL., Jia G., Kelly JD., Kudrna D., Lee R., Richard MM.,
Miklas PN., Osorno JM., Rodrigues J., Thareau V., Urrea
CA., Wang M., Yu Y., Zhang M., Wing RA., Cregan PB.,
Rokhsar DS and Jackson SA., 2014. A reference genome
for common bean and genome-wide analysis of dual
domestications. Nature Genetics, 46: 707-13.
- Sönmez F., Gülser F., Karaca S and Gökkaya T., 2018.
Determination of some physical and chemical properties
of research area soils of Bolu Abant İzzet Baysal
University. International Journal of Agricultural and
Wildlife Sciences, 4(1): 68-78.
- Sperotto RA and Ricachenevsky FK., 2017. Common bean Fe
biofortification using model species’ lessons. Frontiers in
Plant Science, 8: 2187.
- Welch RM and Graham RD., 2004. Breeding for
micronutrients in staple food crops from a human
nutrition perspective. Journal of Experimental Botany, 55:
353-364.
- White PJ and Broadley MR., 2005. Biofortifying crops with
essential mineral elements. Trends in Plant Science, 10:
586-593.
- WHO 2009. Global health risks, mortality and burden of
disease attributable to selected major risks. Geneva,
Switzerland, WHO.
- Yeken MZ., Kantar F., Çancı H., Özer G and Çiftçi V., 2018a.
Breeding of dry bean cultivars using Phaseolus vulgaris
landraces in Turkey. International Journal of Agricultural
and Wildlife Sciences, 4(1): 45-54.
- Yeken MZ., Baloch FS., Nadeem MA., Çiftçi V and Karaköy T.,
2018b. Variation of some seed mineral contents in 236
bean (Phaseolus vulgaris L.) landraces in Turkey.
International Agriculture Congress, 3-6 May, Komrat,
Moldova.
- Yücel C., Baloch FS and Ozkan H., 2009. Genetic analysis of
some physical properties of bread wheat grain (Triticum
aestivum L. em Thell). Turkish Journal of Agriculture and
Forestry, 33: 525-535.