Nezahat TURFAN, Sezgin AYAN, AYSUN PEKŞEN, Şeyma Selin AKIN

Antioxidant Enzyme Activities of Some Wild and Cultivated Edible Mushrooms in Turkey

In this study; wild and cultivated edible mushrooms [Boletus edulisBull.:Fr, Craterellus cornucopioides(L.) Pers., Lactarius deliciosus(L.ex Fr.) S.F.Gray, Laetiporus sulphureus(Bull.:Fr.) Murr., Marasmius oreades(Bolt. ex Fr.) Fr., Morchella conicaPers., Ramaria botrytis(Pers.:Fr.) Ricken, Tricholoma terreum(Schaeff.: Fr.) P.Kumm., Hericium erinaceus(Bull.:Fr.) Pers., Lentinula edodes(Berk.) Pegler, Ganoderma lucidum(Curt.:Fr.) P. Karst., and Pleurotus ostreatus(Jacq. ex Fr.) P. Kumm. (1-4)] were obtained from different locations in Turkey. Phenylalanine ammonia lyase (PAL) enzyme activity, ascorbate peroxidase (APX), peroxidase (POD), and superoxide dismutase (SOD) activity changesand nitrate, β-carotene and lycopene levels were investigated in 15 samples to determine antioxidant enzyme capacity. As a result of the study, the highest amount of β-carotene and lycopene were determined in H. erinaceus.P. ostreatus-2 had the lowest amount of β-carotene, whereas Pleurotus ostreatus-1 had the lowest amount of lycopene. Species rich in nitrate content wereC. cornucopioidesand P. ostreatus-4. P. ostreatus-3 wasthe poorest species in terms of nitrate compared to other mushroom samples. PAL activity of mushrooms varied between 5.863 and 8.893 EU mg-1protein. For APX values, P. ostreatus-4 had the highest value, while H. erinaceusspecies had the lowest value. Among mushroom species, the highest and the lowest POD values were determined in H. erinaceusand B. edulis, respectively. C. cornucopioideshad the highest and P. ostreatus-3had the lowest SOD values.

Türkiye'deki Bazı Doğa ve Kültürü Yapılan Yenebilir MantarlarınAntioksidan Enzim Aktiviteleri

Bu çalışmada; Türkiye’nin farklı yörelerinden temin edilen 15 doğa ve kültür mantar türüne [Boletus edulisBull.:Fr, (Ayı mantarı), Craterellus cornucopioides(L.) Pers. (Borazan mantarı), Lactarius deliciosus(L. ex Fr.) S.F.Gray (Kanlıca mantarı), Laetiporus sulphureus(Bull.: Fr.) Murr. (Kükürt mantarı), Marasmius oreades(Bolt. ex Fr.) Fr. (Cincile mantarı), Morchella conica Pers. (Kuzugöbeği mantarı), Ramaria botrytis(Pers.:Fr.) Ricken (Pürpürüm mantarı), Tricholoma terreum (Schaeff.: Fr.) P.Kumm. (Karakız mantarı), Hericium erinaceus(Bull.: Fr.) Pers. (Aslan yelesi mantarı), Lentinula edodes(Berk.) Pegler(Meşe mantarı), Ganoderma lucidum(Curt.: Fr.) P. Karst. (Reishi mantarı) ve Pleurotus ostreatus(Jacq. ex Fr.) P. Kumm. (1-4) (Kayın mantarı)] aitörnekteantioksidan enzim kapasitesini belirlemek için fenilalanin amonyum liyaz (PAL)enzim aktivitesi,askorbat peroksidaz (APX), peroksidaz (POD) ve süperoksid dismutaz (SOD) aktivite değişimlerive nitrat, β-karoten ile likopen düzeyleriaraştırılmıştır. Sonuç olarak, farklı yörelerden temin edilen mantar örnekleri içerisinde en yüksek β-karoten ve likopen miktarı H. erinaceustüründe belirlenmiştir. β-karoten miktarının en düşük olduğu tür P. ostreatus-2 iken en düşük likopen miktarı P. ostreatus-1 türünde saptanmıştır. Nitrat içeriği bakımından zengin olan türler; C. cornucopioidesve P. ostreatus-4 olarak tespit edilmiştir. P. ostreatus-3, diğer mantar örneklerine kıyasla nitrat bakımından en fakir türolarak belirlenmiştir. Mantarların PAL aktivitesi 5.863 ve 8.893 EU mg-1protein arasında değişmiştir. En yüksek APX değerinin P. ostreatus-4 türüne, en düşük değerin ise H. erinaceustürüne ait olduğu bulunmuştur. Mantar türleri arasında en yüksek ve en düşük POD değerleri sırasıyla H. erinaceusve B. edulistürlerinde saptanmıştır. SOD değeri en yüksek türC. cornucopioides, en düşük tür ise P. ostreatus-3olarak belirlenmiştir

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