Yabani Ravent (Rheum ribes L.)’in Doku Kültürü ile Çoğaltım Olanakları Üzerine Araştırma

Rheum ribes L. tıbbi öneme sahip çok yıllık yabani bir bitki türüdür. Bu çalışmada, in vitro koşullarda büyütülmüş 7 günlük fidelerden alınan farklı eksplant tipleri (hipokotil ve kotiledon), Indol-3-butirik asit (IBA) (0.0 ve 1.0 mg L-1) ve 6-Benzilaminopurin (BAP) (0.0, 1.0 ve 2.0 mg L-1) kombinasyonları ilave edilmiş MS (Murashige and Skoog) besin ortamında kültüre alınmıştır. Araştırma sonucunda sürgün ve kallus oluşumu yönünden hipokotil eksplantlarının kotiledon eksplantlarından daha başarılı olduğu belirlenmiştir. Hipokotil eksplantlarında, eksplant başına en yüksek sürgün sayısı (4.0 adet eksplant-1) 2mg L-1 BAP ve 1 mg L-1 IBA ilavesi yapılmış MS ortamından (MS6) elde edilirken, kallus oluşum oranı açısından ise MS4 (MS + 1 mg L-1 BAP + 1 mg L-1 IBA) (% 88.8) ve MS6 (MS + 2 mg L-1 BAP + 1 mg L-1 IBA) (% 83.3) ortamlarının daha başarılı olduğu tespit edilmiştir.

Anahtar Kelimeler:

Rheum ribes L., in vitro rejenerasyon, kallus, sürgün

Research on Regeneration Via Tissue Culture on Wild Rhubarb (Rheum ribes L.)

Rheum ribes L. is a perennial wild medicinal plant. In this study, the different explants types (hypocotyl and cotyledon) taken from 7-days-old plantlets were cultured in MS (Murashige and Skoog) media supplemented with 0.0, 1.0 and 2.0 mg L-1 of 6-Benzylaminopurine (BAP) with 0.0 and 1.0 mg L-1 of Indole-3-butyric acid (IBA). Hypocotyl explants were found to be more successful than cotyledon explants in terms of shoot and callus formation. The highest number of shoots per explant (4.0 shoot explant-1 ) was obtained from MS medium supplemented with 2 mg L-1 BAP and 1 mg L-1 IBA (MS6) from the hypocotyl explants. In terms of the rate of callus formation (%), MS4 (MS + 1 mg L-1 BAP + 1 mg L-1 IBA) (88.8%) and MS6 medium (MS + 2 mg L-1 BAP + 1 mg L-1 IBA) (83.3%) were found to be more successful than the hypocotyl explants.

Keywords:

Rheum ribes L., in vitro regeneration, callus, shoot,

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